製品の概要

  • 製品名

    Anti-CD11c antibody [EP1347Y] - C-terminal
    CD11c 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EP1347Y] to CD11c - C-terminal
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: IHC-Fr, IHC-P, WB, IPmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide within Human CD11c aa 1150 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: P20702

  • ポジティブ・コントロール

    • WB: THP-1 starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate; THP-1 + TPA lysate. IHC-P: Human tonsil, lymph node and spleen tissues. IP: THP-1 starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate.
  • 特記事項

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52632 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/100.
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/100 - 1/250.

WB 1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 128 kDa).
IP 1/20.

For unpurified use at 1/80.

ターゲット情報

  • 機能

    Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis.
  • 組織特異性

    Predominantly expressed in monocytes and granulocytes.
  • 配列類似性

    Belongs to the integrin alpha chain family.
    Contains 7 FG-GAP repeats.
    Contains 1 VWFA domain.
  • ドメイン

    The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
  • 細胞内局在

    Membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • 95 alpha chain antibody
    • 95 antibody
    • CD 11c antibody
    • CD11 antigen like family member C antibody
    • CD11 antigen-like family member C antibody
    • CD11c antibody
    • CD11c antigen antibody
    • Complement component 3 receptor 4 subunit antibody
    • CR4 antibody
    • Integrin alpha X antibody
    • Integrin alpha X chain antibody
    • Integrin alpha-X antibody
    • Integrin aX antibody
    • Integrin subunit alpha X antibody
    • integrin, alpha X (antigen CD11C (p150), alpha polypeptide) antibody
    • integrin, alpha X (complement component 3 receptor 4 subunit antibody
    • ITAX_HUMAN antibody
    • ITGAX antibody
    • LEU M5 alpha subunit antibody
    • Leu M5 antibody
    • Leukocyte adhesion glycoprotein p150 95 alpha chain antibody
    • Leukocyte adhesion glycoprotein p150 antibody
    • Leukocyte adhesion receptor p150 95 antibody
    • Leukocyte adhesion receptor p150 antibody
    • Leukocyte surface antigen p150 95 alpha subunit antibody
    • Leukocyte surface antigen p150 alpha subunit antibody
    • Myeloid membrane antigen alpha subunit antibody
    • p150 95 integrin alpha chain antibody
    • p150 antibody
    • p150/95 antibody
    • SLEB6 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling CD11c with Purified ab52632 at 1:500 dilution (0.21 μg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • All lanes : Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (purified)

    Lane 1 : Untreated THP-1 (Human monocytic leukemia monocyte) whole cell lysates
    Lane 2 : THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 128 kDa



    Blocking and diluting buffer: 5% NFDM/TBST

  • ab52632 (purified) at 1:20 dilution (2µg) immunoprecipitating CD11c in THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate.

    Lane 1 (input): THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate 10ug
    Lane 2 (+): ab52632 & THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52632 in THP-1 (Human monocytic leukemia monocyte) starved for 2 hours, then treated with Phorbol-12-myristate-13-acetate at 100ng/ml for 96 hours whole cell lysate

    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1:1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST."

  • Immunohistochemical analysis of frozen human liver tissue sections staining CD11c with ab52632 at 1/100 dilution. Alexa Fluor® 647 goat anti-rabbit secondary antibod ywas used. DAPI was used as a nuclear counterstain.

  • Immunohsitochemical analysis of paraffin-embedded human tonsil tissue labelling CD11c with ab52632 at 1/500 dilution. Tissue fixed with 10% NBF. An Alexa Fluor® 647 Goat anti-Rabbit IgG secondary antibody was used. Heat mediated antigen retrieval with Tris/EDTA, ph 9 carried out.

  • Unpurified ab52632 showing positive staining in Normal tonsil tissue.

  • Unpurified ab52632 at 1/400 dilution staining CD11c in human tonsil tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed, prior to blocking in 10% BSA in FCS (20ml) + DMEM (80ml) for 30 minutes at 21°C and then incubated with ab52632 for 20 hours at 4°C. A biotin conjugated rabbit polyclonal to mouse Ig, diluted 1/400, was used as the secondary antibody.

    See Abreview

  • Unpurified ab52632 showing negative staining in Normal brain tissue.

  • All lanes : Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (unpurified)

    Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
    Lane 2 : THP1 (Human acute monocytic leukemia cell line) + PMA Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 128 kDa
    Observed band size: 150 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab52632 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  • Anti-CD11c antibody [EP1347Y] - C-terminal (ab52632) at 1/1000 dilution (unpurified) + THP-1 + TPA lysate at 10 µg

    Secondary
    Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 128 kDa
    Observed band size: 150 kDa why is the actual band size different from the predicted?

  • Unpurified ab52632 showing negative staining in Normal breast tissue.

  • Unpurified ab52632 showing negative staining in Skeletal muscle tissue.

  • Unpurified ab52632 showing positive staining in Normal spleen tissue.

参考文献

This product has been referenced in:

  • Kashiwazaki D  et al. Efficacy of Carotid Endarterectomy for Mild (<50%) Symptomatic Carotid Stenosis with Unstable Plaque. World Neurosurg 121:e60-e69 (2019). Read more (PubMed: 30244188) »
  • Mackiewicz Z  et al. Sjögren's Syndrome: Concerted Triggering of Sicca Conditions. J Immunol Res 2019:2075803 (2019). Read more (PubMed: 30723748) »
See all 34 Publications for this product

レビューと Q&A

1-10 of 26 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (tonsil)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris/EDTA pH 9.0
Permeabilization
No
Specification
tonsil
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 15% · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Aug 12 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (mouse tumor)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: acetic and EDTA tested
Permeabilization
No
Specification
mouse tumor
Blocking step
Serum as blocking agent for 8 minute(s) · Concentration: 0µg/mL · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Jul 18 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH9
Permeabilization
No
Specification
Liver
Blocking step
No blocking step used for 10 minute(s) · Temperature: RT°C
Fixative
10% NBF

Abcam user community

Verified customer

投稿 Apr 30 2019

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

投稿 May 09 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Skin)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate pH 6
Permeabilization
No
Specification
Skin
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 May 09 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human Sarcoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH6
Permeabilization
No
Specification
Human Sarcoma
Blocking step
Innovex Background Buster as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Nov 09 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Lymph node)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA
Permeabilization
No
Specification
Lymph node
Blocking step
Casein as blocking agent for 15 minute(s) · Concentration: 0.25% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Aug 03 2015

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Antigen retrieval step
Heat mediated
Sample
Rabbit Tissue sections (Tonsil)
Specification
Tonsil
Permeabilization
Yes - 0.2% Tween in PBS
Fixative
Formaldehyde

Abcam user community

Verified customer

投稿 Nov 25 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer, pH 6.0
Sample
Cynomolgus Monkey Tissue sections (Tonsil, lymph node, spleen, thymus)
Specification
Tonsil, lymph node, spleen, thymus
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: Rt°C
Fixative
Formaldehyde

Jing Ma

Verified customer

投稿 Dec 18 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 16.7% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate pH6.1
Sample
Human Tissue sections (colon)
Specification
colon
Permeabilization
No
Fixative
Formaldehyde

Ms. Judith Gras

Verified customer

投稿 Sep 24 2013

1-10 of 26 Abreviews or Q&A

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