Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (bone)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization
Yes - tween 0.1%
Specification
bone
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA 0.1% T-PBS
Dilution
1/500
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Osteosarcoma infiltrating neutrophils are stained with CD11b PE/Cy5 in the above mentioned conditions. The CD11b staining collocalized with neutrophil elastase specific neutrophil enzyme.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Szilvia Baron
Verified customer
投稿 Jan 21 2019