製品名Anti-CD11b antibody [M1/70] (PE/Cy5®)
CD11b 一次抗体 製品一覧
製品の詳細Rat monoclonal [M1/70] to CD11b (PE/Cy5®)
標識PE/Cy5®. Ex: 496nm, Em: 670nm
アプリケーション適用あり: Flow Cytmore details
種交差性交差種: Mouse, Rat, Rabbit, Dog, Human, Pig, Monkey
Tissue/ cell preparation (Mouse) - C57BL/10 mouse splenic T cells and concanavalin A activated C57BL/10 splenocytes.
- Mouse spleen cells.
特記事項This product or portions thereof is manufactured under license from Carnegie Mellon University under U.S. Patent Number 5,268,486 and related patents. Cy and CyDye are trademarks of GE Healthcare Limited.
保存方法Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide
Constituents: 0.1% Gelatin, 0.14% Monobasic dihydrogen sodium phosphate, 0.87% Sodium chloride
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- Anti-CD11b antibody [M1/70] (Alexa Fluor® 488) (ab197701)
- Anti-CD11b antibody [M1/70] (Alexa Fluor® 647) (ab197702)
- Anti-CD11b antibody [M1/70] (Alexa Fluor® 405) (ab197703)
- Anti-CD11b antibody [M1/70] (PerCP/Cy5.5®) (ab210299)
- Anti-CD11b antibody [M1/70] - BSA and Azide free (ab212506)
- Anti-CD11b antibody [M1/70] (PE/Cy7 ®) (ab218786)
- Anti-CD11b antibody [M1/70] (PerCP) (ab237025)
- Anti-CD11b antibody [M1/70] - BSA and Azide free (ab237965)
- Anti-CD11b antibody [M1/70] (violetFluor™ 450) (ab242266)
- Anti-CD11b antibody [M1/70] (FITC) (ab24874)
- Anti-CD11b antibody [M1/70] (Phycoerythrin) (ab25175)
- Anti-CD11b antibody [M1/70] (redFluor™ 710) (ab253091)
- Anti-CD11b antibody [M1/70] (Allophycocyanin) (ab25482)
- Anti-CD11b antibody [M1/70] (Allophycocyanin/Cy7 ®) (ab79096)
- Anti-CD11b antibody [M1/70] (ab8878)
Our Abpromise guarantee covers the use of ab25533 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use at an assay dependent concentration.|
機能Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain.
組織特異性Predominantly expressed in monocytes and granulocytes.
関連疾患Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system.
配列類似性Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain.
ドメインThe integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
- Information by UniProt
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
Flow Cytometry analysis of C57Bl/6 bone marrow cells labeling CD11b with ab25533 at 0.125 µg (solid line) or Rat IgG2b (PE/Cy5®) isotype control at 0.125 µg (dashed line).
ab25533 staining CD11b in Human leukocytes by Flow Cytometry. Cells were prepared for staining after red blood cell lysis and were fixed in paraformaldehyde. The sample was incubated with the primary antibody (1/50 in PBS/BSA 1%) for 30 minutes at 4°C. Gating strategy: Neutrophils.
Scatter plot showing mouse blood leukocyte cells stained with ab25533 (green). The cells were harvested from blood drawn from mice and prepared in PBS/BSA 1%. Cells were fixed in formaldehyde. The cells were then incubated in 1x PBS / 1% BSA to block non-specific protein-protein interactions followed by the antibody (1/100) for 30 min at 4ºC. Isotype control antibody (red) was used under the same conditions.
ab25533 staining CD11b in Rat splenocytes by Flow Cytometry. Rat spleen was homogenized and red blood cells were lysed in PBS + 1% BSA and 0.01% sodium azide. The sample was incubated with the primary antibody (1/100 in PBS + 1% BSA and 0.01% sodium azide) for 1 hour at 4°C.
Gating Strategy: Live Macrophages.
This product has been referenced in:
- Wen G et al. Genetic and Pharmacologic Inhibition of the Neutrophil Elastase Inhibits Experimental Atherosclerosis. J Am Heart Assoc 7:N/A (2018). Read more (PubMed: 29437605) »
- Xu J et al. Cobalt (II) ions and nanoparticles induce macrophage retention by ROS-mediated down-regulation of RhoA expression. Acta Biomater 72:434-446 (2018). Flow Cyt . Read more (PubMed: 29649639) »