APC Anti-CD11b 抗体 [EP1345Y] (ab221248)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- APC Rabbit monoclonal [EP1345Y] to CD11b
- Suitable for: Flow Cyt (Intra)
- Reacts with: Human
- Conjugation: APC. Ex: 645nm, Em: 660nm
Related conjugates and formulations
製品の概要
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製品名
APC Anti-CD11b antibody [EP1345Y]
CD11b 一次抗体 製品一覧 -
製品の詳細
APC Rabbit monoclonal [EP1345Y] to CD11b -
由来種
Rabbit -
標識
APC. Ex: 645nm, Em: 660nm -
アプリケーション
適用あり: Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt (Intra): human monocytes
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Store In the Dark. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1345Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-CD11b antibody [EP1345Y] - BSA and Azide free (ab187537)
- PE Anti-CD11b antibody [EP1345Y] (ab213186)
- Alexa Fluor® 647 Anti-CD11b antibody [EP1345Y] - C-terminal (ab311113)
- Alexa Fluor® 568 Anti-CD11b antibody [EP1345Y] - C-terminal (ab313012)
- Alexa Fluor® 555 Anti-CD11b antibody [EP1345Y] - C-terminal (ab313216)
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab221248の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Flow Cyt (Intra) |
1/500.
The cellular localisation of this product has been verified in ICC/IF. |
特記事項 |
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Flow Cyt (Intra)
1/500. The cellular localisation of this product has been verified in ICC/IF. |
ターゲット情報
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機能
Integrin alpha-M/beta-2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin alpha-M/beta-2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. -
組織特異性
Predominantly expressed in monocytes and granulocytes. -
関連疾患
Genetic variations in ITGAM has been associated with susceptibility to systemic lupus erythematosus type 6 (SLEB6) [MIM:609939]. Systemic lupus erythematosus (SLE) is a chronic, inflammatory and often febrile multisystemic disorder of connective tissue. It affects principally the skin, joints, kidneys and serosal membranes. It is thought to represent a failure of the regulatory mechanisms of the autoimmune system. -
配列類似性
Belongs to the integrin alpha chain family.
Contains 7 FG-GAP repeats.
Contains 1 VWFA domain. -
ドメイン
The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3684 Human
- Omim: 120980 Human
- SwissProt: P11215 Human
- Unigene: 172631 Human
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別名
- antigen CD11b (p170) antibody
- Antigen CD11b p170 antibody
- CD11 antigen like family member B antibody
see all
画像
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Overlay histogram showing human CD14+ monocytes stained with ab221248 (red line). Human peripheral blood mononuclear cells were incubated for 30min on ice in 1x PBS containing 10ug/ml human IgG and 10% normal goat serum to block FC receptors and non-specific protein-protein interactions. Then cells were surface stained for CD14 FITC (ab28061) for 30 min on ice. The cells were then permeabilised with 4.2% formaldehyde and 0.1% saponin for 20 min at 4°C. After cells were blocked with 1xPBS containing 0.1% saponin and 10% normal goat serum for 30 min on ice followed by the antibody (ab221248, 1x106 cells in 100ul at 1ug/ml) for 30 min on ice.
Isotype control antibody (black line) Rabbit IgG (monoclonal) Allophycocyanin (ab232814) used at the same concentration and conditions as the primary antibody. CD14 FITC single stained cells (blue line) were also used as a control.
Acquisition of >5,000 events were collected using a 40 mW Red laser (640nm) and 670/14 bandpass filter. Events were gated on live single cell CD14+ positive monocytes.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab221248 は論文での使用が確認できていません。