Anti-CD105 抗体 [MJ7/18] (ab81456)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rat monoclonal [MJ7/18] to CD105
- Suitable for: IHC-Fr, IP, Flow Cyt, WB
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-CD105 antibody [MJ7/18]
CD105 一次抗体 製品一覧 -
製品の詳細
Rat monoclonal [MJ7/18] to CD105 -
由来種
Rat -
アプリケーション
適用あり: IHC-Fr, IP, Flow Cyt, WBmore details -
種交差性
交差種: Mouse -
免疫原
Tissue, cells or virus corresponding to Mouse CD105. Inflamed mouse skin
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ポジティブ・コントロール
- WB: Mouse lung and liver tissue lysates Flow Cyt: bEND.3
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特記事項
This product was switched from a hybridoma to a recombinant production format on 27th October 2021.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
ポリ/モノ
モノクローナル -
クローン名
MJ7/18 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81456の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-Fr |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
1/10 - 1/20.
ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 1/10 - 1/20. Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 70 kDa.
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特記事項 |
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IHC-Fr
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
Flow Cyt
1/10 - 1/20. ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody. 1/10 - 1/20. Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 70 kDa. |
ターゲット情報
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機能
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
組織特異性
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
関連疾患
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 13805 Mouse
- SwissProt: Q63961 Mouse
- Unigene: 225297 Mouse
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別名
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
画像
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All lanes : Anti-CD105 antibody [MJ7/18] (ab81456) at 5 µg/ml
Lane 1 : Mouse Lung tissue lysate with 3% Milk
Lane 2 : Mouse Liver tissue lysate with 3% Milk
Lane 3 : NIH/3T3 whole cell lysate with 3% Milk
Lysates/proteins at 40 µg per lane.
Secondary
All lanes : Rabbit monoclonal [EPR16891] to GAPDH - Loading Control (ab181602)
Predicted band size: 70 kDa
Observed band size: 80 kDa why is the actual band size different from the predicted?Lanes 1 - 3: Merged signal (red and green). Green - ab81456 observed at 80 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.
ab81456 was shown to react with CD105 in Western blot.
Membranes were blocked in 3% milk before incubation with ab81456 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 5 µg/ml and a 1 in 20000 dilution respectively.
Blots were incubated with Goat anti-Rat IgG H&L (IRDye® 800CW) preabsorbed (ab253031) and Goat anti-Rabbit IgG H&L (IRDye$®$ 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Additional band(s) were observed at 45 kDa, these band(s) may represent alternative splice variants. This has not been investigated further.
Lane 1: Mouse Lung tissue lysate (40 µg)
Lane 2: Mouse Liver tissue lysate (40 µg)
Lane 3: NIH/3T3 whole cell lysate (40 µg) -
Flow cytometry overlay histogram showing left bEND.3 positive cells and right negative NIH3T3 cells stained with ab81456 (red line). The cells were incubated in 1x PBS containing 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab81456) (1x106 in 100 μl at 1 μg/ml) for 30 min on ice.
The secondary antibody Goat anti-rat IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150165) was used at 1/2000 for 30 min on ice.
Isotype control antibody (black line) was Rat IgG2aκ (ab18450) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab81456 は 1 報の論文で使用されています。
- Ntokou A et al. Characterization of the platelet-derived growth factor receptor-a-positive cell lineage during murine late lung development. Am J Physiol Lung Cell Mol Physiol 309:L942-58 (2015). PubMed: 26320158