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    cd105-antibody-mem-226-ab2529.pdf

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Immunology Cell Type Markers CD Endothelial Cells
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遺伝子ノックアウト細胞株 検証済

Anti-CD105 抗体 [MEM-226] (ab2529)

  • Datasheet
  • SDS
Submit a review Q&A (2)References (13)

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
  • Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)
  • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)
  • Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)
  • Western blot - Anti-CD105 antibody [MEM-226] (ab2529)

Key features and details

  • Mouse monoclonal [MEM-226] to CD105
  • Suitable for: ICC/IF, Sandwich ELISA, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Rat, Human, Recombinant fragment
  • Isotype: IgG1

こちらの製品もご検討ください

標識
Product image
Alexa Fluor® 488 Conjugation Kit (Fast) - Lightning-Link® (ab236553)
一次抗体
APC Anti-CD105 antibody [MEM-226], prediluted (ab60902)

関連製品

製品の概要

  • 製品名

    Anti-CD105 antibody [MEM-226]
    CD105 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [MEM-226] to CD105
  • 由来種

    Mouse
  • 特異性

    This antibody recognises CD105 antigen.
  • アプリケーション

    適用あり: ICC/IF, Sandwich ELISA, Flow Cyt, WBmore details
  • 種交差性

    交差種: Rat, Human, Recombinant fragment
  • 免疫原

    Recombinant full length protein (Human). Expressed in vaccinia virus containing CD105 cDNA.

  • ポジティブ・コントロール

    • ICC/IF: HeLa cells. WB: Human colon tissue lysate. Flow Cyt: U937 cells.
  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度

    Protein A purified
  • 特記事項(精製)

    Purified from TCS. Purity >95% by SDS-PAGE.
  • ポリ/モノ

    モノクローナル
  • クローン名

    MEM-226
  • アイソタイプ

    IgG1
  • 研究分野

    • Immunology
    • Cell Type Markers
    • CD
    • Endothelial Cells
    • Stem Cells
    • Mesenchymal Stem Cells
    • Surface Molecules
    • Cancer
    • Tumor biomarkers
    • Other
    • Cardiovascular
    • Vasculature
    • Endothelium
    • Stem Cells
    • Endothelial Progenitors
    • Endothelial Markers
    • Cardiovascular
    • Cardiovascular Markers
    • Cell Markers
    • Endothelial Cells
    • Cardiovascular
    • Angiogenesis
    • Endothelial Cell Markers

関連製品

  • Alternative Versions

    • FITC Anti-CD105 antibody [MEM-226] (ab18278)
    • APC Anti-CD105 antibody [MEM-226], prediluted (ab60902)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant human CD105 protein (ab54338)
  • Related Products

    • Recombinant human CD105 protein (ab54338)
  • sELISA pair antibody

    • Anti-CD105 antibody (ab21224)

アプリケーション

Our Abpromise guarantee covers the use of ab2529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
Sandwich ELISA Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to CD105 (ab21224). For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to CD105 (ab21224) as Detection.
Flow Cyt Use a concentration of 1 - 2 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Use under non reducing condition.

ターゲット情報

  • 機能

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
  • 組織特異性

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.
  • 関連疾患

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
  • 細胞内局在

    Membrane.
  • Target information above from: UniProt accession P17813 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 2022 Human
    • Entrez Gene: 497010 Rat
    • Omim: 131195 Human
    • SwissProt: P17813 Human
    • Unigene: 76753 Human
    • Unigene: 187025 Rat
    • 別名

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    画像

    • Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      All lanes : Anti-CD105 antibody [MEM-226] (ab2529) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : CD105 knockout HeLa whole cell lysate
      Lane 3 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.


      Lanes 1 - 3: Merged signal (red and green). Green - ab2529 observed at 70 kDa. Red - loading control, ab181602, observed at 37 kDa.

      ab2529 was shown to recognize ENG (Endoglin) in wild-type HeLa cells as signal was lost at the expected MW in ENG knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and ENG knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab2529 and ab181602 (Rabbit anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)
      Flow Cytometry - Anti-CD105 antibody [MEM-226] (ab2529)

      Overlay histogram showing U937 (Human histiocytic lymphoma cell line) cells stained with ab2529 (red line).

      The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2529, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in U937 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    • Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)
      Immunocytochemistry/ Immunofluorescence - Anti-CD105 antibody [MEM-226] (ab2529)

      ICC/IF image of ab2529 stained HeLa (Human epithelial cell line from cervix adenocarcinoma) cells.

      The cells were 4% formaldehyde fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1 hour to permeabilize the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2529, 10 µg/ml) overnight at +4°C. The secondary antibody (green) was ab69879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1 hour. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1 hour. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43 µM.

    • Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)
      Sandwich ELISA - Anti-CD105 antibody [MEM-226] (ab2529)
      Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.
    • Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      Western blot - Anti-CD105 antibody [MEM-226] (ab2529)
      Anti-CD105 antibody [MEM-226] (ab2529) at 5 µg/ml + Human colon tissue lysate - total protein (ab30051) at 10 µg

      Secondary
      Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Observed band size: 80 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 45 kDa, 55 kDa. We are unsure as to the identity of these extra bands.

    プロトコール

    • Flow cytometry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols
    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (13)

    ab2529 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2529 は 13 報の論文で使用されています。

    • Chen Y  et al. Differentiation of human adipose derived stem cells into Leydig-like cells with molecular compounds. J Cell Mol Med 23:5956-5969 (2019). PubMed: 31293077
    • Shao B  et al. Regulatory effects of miRNA-181a on FasL expression in bone marrow mesenchymal stem cells and its effect on CD4+T lymphocyte apoptosis in estrogen deficiency-induced osteoporosis. Mol Med Rep 18:920-930 (2018). PubMed: 29845202
    • Rawal S  et al. Integration of mesenchymal stem cells into islet cell spheroids improves long-term viability, but not islet function. Islets 9:87-98 (2017). PubMed: 28662368
    • Sargent A  et al. CNS disease diminishes the therapeutic functionality of bone marrow mesenchymal stem cells. Exp Neurol 295:222-232 (2017). PubMed: 28602834
    • Zhang GP  et al. MicroRNA-98 regulates osteogenic differentiation of human bone mesenchymal stromal cells by targeting BMP2. J Cell Mol Med 21:254-264 (2017). Flow Cyt ; Human . PubMed: 27860183
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Question

    We had recently buy a GPCR EX33 antibody (ab12529) from your company. I tested for FACS (fluorochrme detection with Alexa 488 coupled to anti-rabbit IGg (H+L) secondary antibody). My control was ok, but all cells were detected for GPR EX33 highly, but this must to be detectable highly just after LPS stimulation, and all cells traited with saline was highly expressed for this GPCR. In quantitative PCR, the transcript is very low detectable for the vehicle cells traited with saline and very high with LPS. Also, I was processed to blockade step before primary antibody incubation, with goat serum. Do you have other suggest for the optimal blockade step, because many GPCR are similarity between their recognizing domains, and also should I use a human anti-FCy? Maybe that this antibody is not very specific also (purification) ? I will wait your help,

    Read More

    Abcam community

    Verified customer

    Asked on Dec 14 2005

    Answer

    I'm sorry to hear you are having problems with ab12529; this antibody has not been tested for FACS so it is very difficult to know if the problem can be solved with protocol problems or is due to the antibody not working well in FACS. As the antibody has been tested in IHC-P it means the antibody has the ability to recognise the native form of the protein, however this may require fixation and other protocol changes to get it working. The antibody has been tested in human brain tissue, so I would recommend trying this positive control if possible in IHC-P. The antibody has been purified on a peptide immunogen affinity column so it is purified and recognizes only GPCR EX33. You may be able to decrease the non specific staining with decreasing the antibody concentration and incubation conditions and changing the buffer. Could you please provide us your specific protocol details and we will look into this problem in more detail and hopefully will be able to suggest some tips? I enclose below a link to a protocol questionnaire to help you put your protocol information together easily, we look forward to hearing from you to help you more, https://www.abcam.com/index.html?section=facs&pageconfig=technical&intAbID=12529&mode=questionaire

    Read More

    Abcam Scientific Support

    Answered on Dec 16 2005

    Question

    I am looking for an antibody or antiserum against rat CD105. Thank you very much for your help to find this antibody.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 21 2003

    Answer

    Thank you for your enquiry. Unfortunately, we currently do not have an antibody or antiserum against rat CD105. To see if another company may have what you are looking for, I suggest that you click on the link to "The World's Antibody Gateway". The World's Antibody Gateway is a free search engine service provided by Abcam to help you to quickly find the antibodies that you are looking for. It is a free-text search engine developed by the Abcam team so that it searches the catalogs of all online antibody companies (currently 249). If you have any more questions, please contact us again.

    Read More

    Abcam Scientific Support

    Answered on Nov 21 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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