Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (FFPE Heart section)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 and pH9
Permeabilization
No
Specification
FFPE Heart section
Blocking step
H2O2 as blocking agent for 7 minute(s) · Concentration: 3% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
30 minute(s) · Temperature: 24°C
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Leica Bond polymer detection
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
The antibody worked in both ph6 and ph9 buffer as seen in the image attached.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
In Hwa Um
Verified customer
投稿 Jun 29 2020