Anti-CD105 抗体 [EPR22811-18] (ab231774)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22811-18] to CD105
- Suitable for: Flow Cyt, ICC/IF, IP, IHC-P, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD105 antibody [EPR22811-18]
CD105 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22811-18] to CD105 -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt, ICC/IF, IP, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HUVEC and U937 whole cell lysate; Human lung and placenta tissue lysate. IHC-P: Human ovarian carcinoma and placenta tissue. ICC/IF: U937 cells. Flow Cyt: HUVEC and U937 cells. IP: HUVEC whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22811-18 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab231774の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt |
1/500.
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ICC/IF |
1/500.
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IP |
1/30.
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IHC-P | (1) |
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/1000. Predicted molecular weight: 70 kDa.
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特記事項 |
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Flow Cyt
1/500. |
ICC/IF
1/500. |
IP
1/30. |
IHC-P
1/100. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/1000. Predicted molecular weight: 70 kDa. |
ターゲット情報
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機能
Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors. -
組織特異性
Endoglin is restricted to endothelial cells in all tissues except bone marrow. -
関連疾患
Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2022 Human
- Omim: 131195 Human
- SwissProt: P17813 Human
- Unigene: 76753 Human
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別名
- AI528660 antibody
- AI662476 antibody
- CD 105 antibody
see all
画像
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Anti-CD105 antibody [EPR22811-18] (ab231774) at 1/1000 dilution + U937 (human histiocytic lymphoma monocyte), whole cell lysate 40 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 70 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Exposure time: 103 secondsBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8932339).
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All lanes : Anti-CD105 antibody [EPR22811-18] (ab231774) at 1/1000 dilution
Lane 1 : HUVEC (human umbilical vein endothelial cell), whole cell lysate 20 µg
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate 20 µg
Lane 3 : Raji (human Burkitt's lymphoma B lymphocyte), whole cell lysate 20 µg
Lane 4 : Human lung tissue lysate 20 µg
Lane 5 : Human placenta tissue lysate 20 µg
Secondary
Lanes 1-3 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Lanes 4-5 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 70 kDaBlocking/Diluting buffer and concentration: 5% NFDM/TBST.
Exposure times: Lanes 1-3: 26 secs; Lanes 4-5: 3 mins.
The molecular weight observed is consistent with what has been described in the literature (PMID: 8932339).
This blot was developed using a higher sensitivity ECL substrate.
Negative control: Jurkat (PMID: 28351936); Raji (PMID: 28351936).
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CD105 was immunoprecipitated from 0.35 mg HUVEC (human umbilical vein endothelial cell) whole cell lysate with ab231774 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab231774 1/1000 dilution (0.51 μg/ml). VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.
Lane 1: HUVEC (human umbilical vein endothelial cell) whole cell lysate 10μg
Lane 2: ab231774 IP in HUVEC whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab231774 in HUVEC whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 mins.
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Immunohistochemical analysis of paraffin-embedded human ovarian carcinoma tissue labeling CD105 with ab231774 at 1/100 dilution (5.1 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on endothelial cells of human ovarian carcinoma (PMID: 17502949). The section was incubated with ab231774 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling CD105 with ab231774 at 1/100 dilution (5.1 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human placental trophoblasts (PMID: 17956952) is observed. The section was incubated with ab231774 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
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Immunofluorescent analysis of 100% methanol-fixed U-937 (human histiocytic lymphoma monocyte) cells labeling CD105 with ab231774 at 1/500 dilution, followed by a ab150077 AlexaFluor®488 Goat anti-Rabbit secondary antibody at 1/1000 dilution (Green). Confocal image showing membranous staining in U-937 cells is observed. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.
Negative control: Jurkat (PMID: 28351936).
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Flow cytometric analysis of U-937 (human histiocytic lymphoma monocyte) cells labeling CD105 with ab231774 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. Gated on viable cells.
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Flow cytometric analysis of Jurkat (human T cell leukemia T lymphocyte, Left) / HUVEC (human umbilical vein endothelial cell, Right) cells labeling CD105 with ab231774 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.
Negative control: Jurkat (PMID: 28351936). Gated on viable cells.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (5)
ab231774 は 5 報の論文で使用されています。
- Ye YC et al. Infarct-preconditioning exosomes of umbilical cord mesenchymal stem cells promoted vascular remodeling and neurological recovery after stroke in rats. Stem Cell Res Ther 13:378 (2022). PubMed: 35902882
- Ye G et al. Autophagy-Mediated Activation of Mucosal-Associated Invariant T Cells Driven by Mesenchymal Stem Cell-Derived IL-15. Stem Cell Reports 16:926-939 (2021). PubMed: 33798448
- Ren X et al. Osteogenic ability using porous hydroxyapatite scaffold-based delivery of human placenta-derived mesenchymal stem cells. Exp Ther Med 22:1091 (2021). PubMed: 34504545
- Ye G et al. Oxidative stress-mediated mitochondrial dysfunction facilitates mesenchymal stem cell senescence in ankylosing spondylitis. Cell Death Dis 11:775 (2020). PubMed: 32943613
- Wangzhou K et al. MiR-143-3p Inhibits Osteogenic Differentiation of Human Periodontal Ligament Cells by Targeting KLF5 and Inactivating the Wnt/ß-Catenin Pathway. Front Physiol 11:606967 (2020). PubMed: 33603676