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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-CD105 抗体 [EPR10145-10] (ab170943)

Submit a review Submit a question References (3)
Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
  • Anti-CD105 antibody [EPR10145-10] (ab170943)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10145-10] to CD105
  • Suitable for: WB, IHC-P
  • Knockout validated
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-CD105 antibody [EPR10145-10]
    CD105 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR10145-10] to CD105

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP

  • 種交差性

    交差種: Human
    非交差種: Mouse, Rat

  • 免疫原

    Synthetic peptide within Human CD105 aa 1-200. The exact sequence is proprietary.
    Database link: P17813

  • ポジティブ･コントロール

    • WB: HeLa, HUVEC, and ECV-304 whole cell lysates. IHC-P: Human lung carcinoma, urinary bladder transitional carcinoma, cervical carcinoma, tonsil, uterus and placenta tissues.

  • 特記事項

    According to our internal WB data, ab170943 does not react with mouse and rat.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab170943の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
特記事項
WB
1/1000 - 1/5000. Predicted molecular weight: 70 kDa.
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
追加情報
Is unsuitable for Flow Cyt,ICC/IF or IP.

ターゲット情報

  • 機能

    Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.

  • 組織特異性

    Endoglin is restricted to endothelial cells in all tissues except bone marrow.

  • 関連疾患

    Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.

  • 細胞内局在

    Membrane.
  • Information by UniProt

  • 参照データベース

    • 別名

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    画像

    • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : Wild-type HeLa cell lysate
      Lane 2 : ENG knockout HeLa cell lysate
      Lane 3 : HUVEC cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 20 µg per lane.

      Performed under reducing conditions.

      Predicted band size: 70 kDa
      Observed band size: 70-120 kDa why is the actual band size different from the predicted?



      Lanes 1-4: Merged signal (red and green). Green - ab170943 observed at 70-120 kDa. Red - loading control ab8245 observed at 37 kDa.

       ab170943 Anti-CD105 antibody [EPR10145-10] was shown to specifically react with CD105 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265178 (knockout cell lysate ab256906) was used. Wild-type and CD105 knockout samples were subjected to SDS-PAGE. ab170943 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

       

    • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : Wild-type HeLa whole cell lysate
      Lane 2 : CD105 knockout HeLa whole cell lysate
      Lane 3 : HUVEC whole cell lysate

      Lysates/proteins at 20 µg per lane.

      Predicted band size: 70 kDa



      Lanes 1 - 3: Merged signal (red and green). Green - ab170943 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab170943 was shown to recognize CD105 in wild-type HeLa cells as signal was lost at the expected MW in CD105 knockout cells. Additional cross-reactive bands were observed in the wild-type and knockout cells. Wild-type and CD105  knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% milk. Ab170943 and ab8245 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human lung carcinoma-vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human normal tonsil-vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human urinary bladder transitional carcinoma, vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      ab170943 showing +ve staining in Human normal uterus, vessels tissue.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      Immunohistochemical analysis of paraffin-embedded Human placenta tissue labeling CD105 with ab170943  at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD105 antibody [EPR10145-10] (ab170943)

      Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma tissue labeling CD105 with ab170943 at 1/250 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      Western blot - Anti-CD105 antibody [EPR10145-10] (ab170943)
      All lanes : Anti-CD105 antibody [EPR10145-10] (ab170943) at 1/1000 dilution

      Lane 1 : HUVEC cell lysate
      Lane 2 : ECV-304 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 70 kDa

    • Anti-CD105 antibody [EPR10145-10] (ab170943)
      Anti-CD105 antibody [EPR10145-10] (ab170943)

    参考文献 (3)

    ab170943 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab170943 は 3 報の論文で使用されています。

    • Piao L  et al. Leucine Zipper-EF-Hand Containing Transmembrane Protein 1 Is a Potential Prognostic Biomarker and Promotes Cell Progression in Prostate Cancer. Cancer Manag Res 12:1649-1660 (2020). PubMed: 32184668
    • Piao L  et al. LETM1 is a potential biomarker of prognosis in lung non-small cell carcinoma. BMC Cancer 19:898 (2019). PubMed: 31500591
    • Bychkov A  et al. PSMA expression by microvasculature of thyroid tumors - Potential implications for PSMA theranostics. Sci Rep 7:5202 (2017). PubMed: 28701709

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