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  1. Link

    cd105-antibody-ab21224.pdf

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Immunology Cell Type Markers CD Endothelial Cells
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Anti-CD105 抗体 (ab21224)

  • Datasheet
Reviews (1)Q&A (1)References (2)

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Sandwich ELISA - Anti-CD105 antibody (ab21224)

    Key features and details

    • Rabbit polyclonal to CD105
    • Suitable for: Sandwich ELISA
    • Isotype: IgG

    リコンビナント抗体で、ロット間での高い再現性を実現

    Product image
    Anti-CD105 antibody [EPR19911-259] - BSA and Azide free (Detector) (ab242874)
    • 異なるロット間での安定した再現性
    • 容易なスケールアップ
    • 評価試験による特異性の確認済み
    • 倫理基準に準拠 - アニマル・フリーの生産

    製品の概要

    • 製品名

      Anti-CD105 antibody
      CD105 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to CD105
    • 由来種

      Rabbit
    • アプリケーション

      適用あり: Sandwich ELISAmore details
    • 免疫原

      Highly pure recombinant human soluble CD105 (aas 26-586) bearing C-terminal His 6 tag.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Lyophilized:Reconstitute the antibody (100 micrograms) in 200ul sterile water to >0.5 mg/ml.
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Constituent: PBS
    • Concentration information loading...
    • 精製度

      Protein A purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Immunology
      • Cell Type Markers
      • CD
      • Endothelial Cells
      • Stem Cells
      • Mesenchymal Stem Cells
      • Surface Molecules
      • Cancer
      • Tumor biomarkers
      • Other
      • Cardiovascular
      • Vasculature
      • Endothelium
      • Stem Cells
      • Endothelial Progenitors
      • Endothelial Markers
      • Cardiovascular
      • Cardiovascular Markers
      • Cell Markers
      • Endothelial Cells
      • Cardiovascular
      • Angiogenesis
      • Endothelial Cell Markers

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant human CD105 protein (ab54338)
    • Related Products

      • Recombinant human CD105 protein (ab54338)
    • sELISA pair antibody

      • Anti-CD105 antibody [MEM-226] (ab2529)

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab21224の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    Sandwich ELISA
    Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-226] to CD105 (ab2529). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [MEM-226] to CD105 (ab2529) as Capture.
    特記事項
    Sandwich ELISA
    Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [MEM-226] to CD105 (ab2529). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [MEM-226] to CD105 (ab2529) as Capture.

    ターゲット情報

    • 機能

      Major glycoprotein of vascular endothelium. May play a critical role in the binding of endothelial cells to integrins and/or other RGD receptors.
    • 組織特異性

      Endoglin is restricted to endothelial cells in all tissues except bone marrow.
    • 関連疾患

      Defects in ENG are the cause of hereditary hemorrhagic telangiectasia type 1 (HHT1) [MIM:187300, 108010]; also known as Osler-Rendu-Weber syndrome 1 (ORW1). HHT1 is an autosomal dominant multisystemic vascular dysplasia, characterized by recurrent epistaxis, muco-cutaneous telangiectases, gastro-intestinal hemorrhage, and pulmonary (PAVM), cerebral (CAVM) and hepatic arteriovenous malformations; all secondary manifestations of the underlying vascular dysplasia. Although the first symptom of HHT1 in children is generally nose bleed, there is an important clinical heterogeneity.
    • 細胞内局在

      Membrane.
    • Target information above from: UniProt accession P17813 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 別名

      • AI528660 antibody
      • AI662476 antibody
      • CD 105 antibody
      • CD105 antibody
      • CD105 antigen antibody
      • EGLN_HUMAN antibody
      • END antibody
      • Endoglin antibody
      • Eng antibody
      • FLJ41744 antibody
      • HHT1 antibody
      • ORW antibody
      • ORW1 antibody
      • Osler Rendu Weber syndrome 1 antibody
      • RP11 228B15.2 antibody
      • S endoglin antibody
      • S-endoglin antibody
      • SN6 antibody
      see all

    画像

    • Sandwich ELISA - Anti-CD105 antibody (ab21224)
      Sandwich ELISA - Anti-CD105 antibody (ab21224)
      Standard Curve for CD105 (Analyte: CD105 protein (ab54338)); dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [MEM-226] to CD105 (ab2529) at 5ug/ml and Detector Antibody Rabbit polyclonal to CD105 (ab21224) at 0.5ug/ml.

    プロトコール

    • Protocol Booklet

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet download

      Download

    参考文献 (2)

    ab21224 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab21224 は 2 報の論文で使用されています。

    • Jackson AM  et al. Endothelial cell antibodies associated with novel targets and increased rejection. J Am Soc Nephrol 26:1161-71 (2015). PubMed: 25381426
    • Xu D  et al. Enabling autologous human liver regeneration with differentiated adipocyte stem cells. Cell Transplant 23:1573-84 (2014). PubMed: 24148223

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-2 of 2 Abreviews or Q&A

    Flow Cytometry abreview for Anti-CD105 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Flow Cytometry
    Sample
    Human Cell (endothelial precursor cell)
    Specification
    endothelial precursor cell
    Fixation
    none
    Permeabilization
    No
    Gating Strategy
    CD133 and CD202B
    Read More

    DR. A Jackson

    Verified customer

    投稿 Dec 05 2011

    Question

    BATCH NUMBER 248488 ORDER NUMBER 2502599 DESCRIPTION OF THE PROBLEM no bands SAMPLE human tumor tissue and normal tissue PRIMARY ANTIBODY ab21224 DETECTION METHOD ECL PLUS POSITIVE AND NEGATIVE CONTROLS USED NO CONTROL, ANTIBODY STORAGE CONDITIONS 4C, in pbs SAMPLE PREPARATION grind in liquid nitrogen, resolved in PBS buffer AMOUNT OF PROTEIN LOADED 50ug ELECTROPHORESIS/GEL CONDITIONS reduced, 10% SDS-PAGE TRANSFER AND BLOCKING CONDITIONS TRIS-GLYCINE transfer buffer, 1.5h. 5% NF MILK BLOCKING 2 h. SECONDARY ANTIBODY goat anti-rabbit IgG HRP HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES OUR PROTOCOL WORK VERY WELL WITH OTHER ANTIBODIES. AND OUR FROZEN IHC either no results with this antibody

    Read More

    Abcam community

    Verified customer

    Asked on Jun 07 2007

    Answer

    Thank you for your enquiry. I am sorry to hear that you are experiencing difficulties with this product ab21224 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. After looking at the protocols you used, I am surprised that even using 50ug of protein did not manage to detect any bands. However, it is very hard for me to determine the cause without more details of your WB protocol. If you would be so kind to clarify the following items, it will certainly help me better understand the root of the cause and as a result, I will be able to tag this product as faulty. - did you used a lysis buffer? Was it as strong as RIPA buffer? Please try RIPA buffer as it is the strongest and recommend for extracting membrane bound proteins. - could the no signal you are experiencing be due to poor transfer of protein to membrane? Did you check the transfer efficiency? This can be simply done with a reversible stain such as Ponceau S. - At Abcam, almost all of our products has been tested with 5% BSA. Sometimes, a different blocking buffer might cause cross reaction between the blocking agent and antibodies, therefore causing no bands to appear. I would recommend you try 5% BSA, if you have not already done so. - can you confirm that the second antibody you used also managed to produce good results with other primary antibodies? What was the dilution you used? - was the incubation of the primary antibody done overnight at 4oC? - what type of membrane did you used? - can you confirm the washing buffer used? Was the washing done 15min x 3 times? This is because over washing will eventually cause you to lose signals. I am sorry for the string of questions but I hope the above recommendations may already help you. If you have already tried the above suggestions and still experience problems, please do not hesitate to contact me with details of your order (contact information, shipping address/purchasing agent, etc.). The purchase number you gave was not found in our system. It should be a 6 digit number. Also, please advice me on how you would like to proceed with your enquiry, so that I can immediately arrange for a replacement or refund for you.

    Read More

    Abcam Scientific Support

    Answered on Jun 07 2007

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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