Anti-CD10 抗体 [EPR5904-110] - BSA and Azide free (ab271985)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5904-110] to CD10 - BSA and Azide free
- Suitable for: IP, IHC-P, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CD10 antibody [EPR5904-110] - BSA and Azide free
CD10 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5904-110] to CD10 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IP, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: LNCaP, Raji and Ramos whole cell lysates; Human fetal kidney lysate. IHC-P: Human kidney and breast cancer tissues. IP: LNCaP whole cell lysate.
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特記事項
ab271985 is the carrier-free version of ab208778.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5904-110 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab271985の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IP |
1/20.
|
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IHC-P |
1/4000.
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|
WB |
1/1000. Predicted molecular weight: 85 kDa.
|
特記事項 |
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IP
1/20. |
IHC-P
1/4000. |
WB
1/1000. Predicted molecular weight: 85 kDa. |
ターゲット情報
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機能
Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers. -
配列類似性
Belongs to the peptidase M13 family. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 4311 Human
- Omim: 120520 Human
- SwissProt: P08473 Human
- Unigene: 307734 Human
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別名
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
画像
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Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/10000 dilution + Human fetal kidney lysate at 10 µg
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/10000 dilution
Predicted band size: 85 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 5 secondsThis data was developed using ab208778, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-CD10 antibody [EPR5904-110] (ab208778) at 1/1000 dilution
Lane 1 : LNCaP (Human prostate cancer cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : Ramos (Human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 85 kDa
Observed band size: 90-110 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsThis data was developed using ab208778, the same antibody clone in a different buffer formulation.
Blocking/Dilution buffer: 5% NFDM/TBST.
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Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Surface membrane staining was found in the glomerular epithelium and proximal tubular cells of the Human kidney. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778). -
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CD10 with ab208778 at 1/4000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Membrane staining was found in the subset cells of the Human breast cancer. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778). -
CD10 was immunoprecipitated from 1mg of LNCaP (Human prostate cancer cell line) whole cell lysate with ab208778 at 1/20 dilution.
Western blot was performed from the immunoprecipitate using ab208778 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: LNCaP whole cell lysate, 10µg (Input).
Lane 2: ab208778 IP in LNCaP whole cell lysate.
Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab208778 in LNCaP whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab208778).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab271985 は論文での使用が確認できていません。