製品の概要
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製品名Anti-CD10 antibody [56C6]
CD10 一次抗体 製品一覧 -
製品の詳細Mouse monoclonal [56C6] to CD10
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由来種Mouse
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アプリケーション適用あり: WB, IHC-Pmore details
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種交差性交差種: Cat, Dog, Human
交差が予測される動物種: Mouse
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免疫原
Prokaryotic recombinant fusion protein corresponding to the external of the CD10 glycoprotein
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ポジティブ･コントロール
- IHC-P: MDA-MB-231 human breast cancer cells grown in mouse. Renal cell carcinoma tissue.
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特記事項
We have mixed customer feedback about the mouse specificity, which is why we no longer guarantee this species. Contact scientific support team for any inquiry.
This antibody works well when used in combination with ab972 - Antigen retrieval solution, heat mediated high pH 9.5.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.1% Sodium Azide
Constituents: PBS, Carrier protein, Renoir Red Diluent, pH 7.3 -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名56C6
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アイソタイプIgG1
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研究分野
関連製品
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Antibody Diluent
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Compatible Secondaries
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Immunohistochemistry kits
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Unmasking reagent
アプリケーション
Our Abpromise guarantee covers the use of ab951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000. Predicted molecular weight: 85 kDa. PubMed: 26074811 | |
| IHC-P | 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. For an ABC system, incubate for 30-60 minutes at RT. With high pH retrieval solutions, some tissues may require an avidin-biotin block. Overstaining with hematoxylin can mask staining, thus reducing overall contrast and making interpretation difficult. It is therefore recommended that a light hematoxylin stain be applied. Recently, CD10 has been used in a panel for mantle cell lymphoma with cyclin D1(+), CD43 (+), CD5 (+), IgM (+), CD23 (-) and CD10 (-). |
ターゲット情報
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機能Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers.
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配列類似性Belongs to the peptidase M13 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 4311 Human
- Entrez Gene: 17380 Mouse
- Omim: 120520 Human
- SwissProt: P08473 Human
- SwissProt: Q61391 Mouse
- Unigene: 307734 Human
- Unigene: 296022 Mouse
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別名
- Atriopeptidase antibody
- CALLA antibody
- CD10 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)](https://www.abcam.co.jp/ps/products/0/ab951/Images/ab951-284423-ab9513331.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)Paraffin embedded renal cell carcinoma tissue stained for CD10 with ab951 (1/25 dilution) in immunohistochemical analysis.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)](https://www.abcam.co.jp/ps/products/0/ab951/Images/CD10-Primary-antibodies-ab951-3.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD10 antibody [56C6] (ab951)This image is courtesy of an anonymous Abreview.ab951 at 1/20 dilution staining CD10 in MDA-MB-231 human breast cancer cells grown in mouse by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were paraformaldehyde fixed prior to blocking in 10% casein for 30 minutes at 22°C and then incubated with ab951 for 17 hours at 4°C. A rabbit polyclonal to mouse Ig, diluted 1/200 was used as the secondary antibody.
参考文献
This product has been referenced in:
- Li X et al. Encapsulated papillary carcinoma of the breast: A clinicopathological study of 49 cases. Curr Probl Cancer N/A:N/A (2018). Read more (PubMed: 29731165) »
- Zhu L et al. Surgical monotherapy may be a suitable therapeutic strategy for advanced collecting (Bellini) duct carcinoma: A case report and literature review. Exp Ther Med 12:1181-1184 (2016). IHC-P ; Human . Read more (PubMed: 27446340) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"