Anti-CCR9 抗体 (ab1662)

Submit a review Q&A (2)References (6)

製品の概要

  • 製品名

    Anti-CCR9 antibody
    CCR9 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to CCR9

  • 由来種

    Goat

  • 特異性

    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.

  • アプリケーション

    適用あり: IHC-FoFr, Flow Cyt, ELISA, IHC-Pmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Human

  • 免疫原

    Synthetic peptide:

    IPGMFDDFSYDSTASTDDYMNLNFSSFF

    , corresponding to amino acids 10-37 of Mouse CCR9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Mouse thymus or lymphnodes.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/500 - 1/1000. After perfusion place in 30% sucrose overnight.
Flow Cyt 1/200.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
ELISA 1/100000.
IHC-P 1/250.
  • 追加情報
    Is unsuitable for WB.

    • ターゲット情報

    • 関連性

      The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.

    • 細胞内局在

      Cell membrane; Multi-pass membrane protein.

    • 参照データベース

      • 別名

        • C C chemokine receptor D6 antibody
        • C C chemokine receptor type 9 antibody
        • C C CKR 9 antibody
        • CC Chemokine receptor CCR10 antibody
        • CC CKR 9 antibody
        • CCBP2 antibody
        • CCCKR9 antibody
        • CCR 9 antibody
        • CCR9 antibody
        • CDw199 antibody
        • CDw199 antigen antibody
        • Chemokine (C C motif) receptor 9 antibody
        • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
        • Chemokine (C-C motif) receptor 9 isoform A antibody
        • Chemokine (C-C motif) receptor 9 isoform B antibody
        • Chemokine C C motif receptor 9 antibody
        • Chemokine C C receptor 10 antibody
        • Chemokine CC Motif Receptor 9 antibody
        • Chemokine receptor CCR 9 antibody
        • Chemokine receptor CCR9 antibody
        • Chemokine-binding protein D6 antibody
        • Cmkbr10 antibody
        • CMKBR9 antibody
        • G protein coupled receptor 28 antibody
        • GPR 28 antibody
        • GPR 9 6 antibody
        • GPR28 antibody
        • GPR96 antibody
        • OTTHUMP00000164653 antibody
        • OTTHUMP00000164654 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody (ab1662)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody (ab1662)
        Immonuhistochemistry on mouse spleen, at 1/250 dilution.

      参考文献

      ab1662 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1662 は 6 報の論文で使用されています。

      • Huang Y  et al. Abrogation of CC chemokine receptor 9 ameliorates ventricular remodeling in mice after myocardial infarction. Sci Rep 6:32660 (2016). PubMed: 27585634
      • Chu PS  et al. C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice. Hepatology 58:337-50 (2013). PubMed: 23460364
      • Li J  et al. Anti-CCL25 antibody prolongs skin allograft survival by blocking CCR9 expression and impairing splenic T-cell function. Arch Immunol Ther Exp (Warsz) 61:237-44 (2013). PubMed: 23456208
      • Deutsch AJ  et al. Chemokine receptors in gastric MALT lymphoma: loss of CXCR4 and upregulation of CXCR7 is associated with progression to diffuse large B-cell lymphoma. Mod Pathol : (2012). IHC ; Human . PubMed: 22936065
      • Liu JX  et al. CCR7, CCR8, CCR9 and CCR10 in the mouse hippocampal CA1 area and the dentate gyrus during and after pilocarpine-induced status epilepticus. J Neurochem 100:1072-88 (2007). PubMed: 17181556
      • Marski M  et al. CD18 is required for intestinal T cell responses at multiple immune checkpoints. J Immunol 178:2104-12 (2007). Flow Cyt ; Mouse . PubMed: 17277114

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      Question

      For each of these antibodies, specifically what type of antigen retrieval was used for IHC-formalin fixed, paraffin embedded sections?

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      Answer

      Thank you for your patience. The originator of ab12953 and ab13396 has gotten back to me with the standard IHC protocol that they follow which I have included below. If you have any additional questions, please let me know. Tissue Preparation: Formalin fixation and embedding in paraffin wax Tissue Sectioning: Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C. Deparaffinization: Wash dry slides in 3 changes of xylene – 5 minutes each @ RT Rehydration: Wash slides in 3 changes of 100% alcohol – 3 minutes each @ RT Wash slides in 2 changes of 95% alcohol – 3 minutes each @ RT Wash slides in 1 change of 80% alcohol – 3 minutes @ RT Rinse slides in gentle running distilled water – 5 minutes @ RT Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute @ RT Immunostaining: (Do not allow tissues to dry at any time during the staining procedure) Apply a universal protein block – 20 minutes @ RT Drain protein block from slides, apply diluted primary antibody – 45 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply a biotinylated anti-rabbit IgG (H+L) secondary – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase streptavidin – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase chromogen substrate – 30 minutes @ RT Wash slides in distilled water – 1 minute @ RT Dehydrate: (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) Wash slides in 2 changes of 80% alcohol – 1 minute each @ RT Wash slides in 2 changes of 95% alcohol – 1 minute each @ RT Wash slides in 3 changes of 100% alcohol – 1 minute each @ RT Wash slides in 3 changes of xylene – 1 minute each @ RT Apply coverslip

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      Question

      For each of these antibodies, specifically what type of antigen retrieval was used for IHC-formalin fixed, paraffin embedded sections?

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      Answer

      Thank you for your phone call. For ab13268, ab1662, and ab1669, the originator has informed me that the antigen retrieval method for IHC-formalin fixed, paraffin embedded sections involved treating the sections with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. I will contact you again when I receive information regarding ab12953 and ab13396. Have a great weekend!

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