Anti-CCR9 抗体 (ab1662)

Submit a review Q&A (2)References (7)

Key features and details

  • Goat polyclonal to CCR9
  • Suitable for: IHC-FoFr, Flow Cyt, ELISA, IHC-P
  • Reacts with: Mouse
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-CCR9 antibody
    CCR9 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to CCR9

  • 由来種

    Goat

  • 特異性

    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.

  • アプリケーション

    適用あり: IHC-FoFr, Flow Cyt, ELISA, IHC-Pmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Human

  • 免疫原

    Synthetic peptide:

    IPGMFDDFSYDSTASTDDYMNLNFSSFF

    , corresponding to amino acids 10-37 of Mouse CCR9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Mouse thymus or lymphnodes.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/500 - 1/1000. After perfusion place in 30% sucrose overnight.
Flow Cyt 1/200.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
ELISA 1/100000.
IHC-P 1/250.
  • 追加情報
    Is unsuitable for WB.

    • ターゲット情報

    • 機能

      Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.

    • 組織特異性

      Highly expressed in the thymus and low in lymph nodes and spleen.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • C C chemokine receptor D6 antibody
        • C C chemokine receptor type 9 antibody
        • C C CKR 9 antibody
        • C-C chemokine receptor type 9 antibody
        • C-C CKR-9 antibody
        • CC Chemokine receptor CCR10 antibody
        • CC CKR 9 antibody
        • CC-CKR-9 antibody
        • CCBP2 antibody
        • CCCKR9 antibody
        • CCR 9 antibody
        • CCR-9 antibody
        • Ccr9 antibody
        • CCR9_HUMAN antibody
        • CDw199 antibody
        • CDw199 antigen antibody
        • Chemokine (C C motif) receptor 9 antibody
        • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
        • Chemokine (C-C motif) receptor 9 isoform A antibody
        • Chemokine (C-C motif) receptor 9 isoform B antibody
        • Chemokine C C motif receptor 9 antibody
        • Chemokine C C receptor 10 antibody
        • Chemokine CC Motif Receptor 9 antibody
        • Chemokine receptor CCR 9 antibody
        • Chemokine receptor CCR9 antibody
        • Chemokine-binding protein D6 antibody
        • Cmkbr10 antibody
        • CMKBR9 antibody
        • G protein coupled receptor 28 antibody
        • G-protein coupled receptor 28 antibody
        • GPR 28 antibody
        • GPR 9 6 antibody
        • GPR-9-6 antibody
        • GPR28 antibody
        • GPR96 antibody
        • OTTHUMP00000164653 antibody
        • OTTHUMP00000164654 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody (ab1662)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody (ab1662)
        Immonuhistochemistry on mouse spleen, at 1/250 dilution.

      参考文献 (7)

      ab1662 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1662 は 7 報の論文で使用されています。

      • Niu Y  et al. CCL25 promotes the migration and invasion of non-small cell lung cancer cells by regulating VEGF and MMPs in a CCR9-dependent manner. Exp Ther Med 19:3571-3580 (2020). PubMed: 32346420
      • Huang Y  et al. Abrogation of CC chemokine receptor 9 ameliorates ventricular remodeling in mice after myocardial infarction. Sci Rep 6:32660 (2016). PubMed: 27585634
      • Chu PS  et al. C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice. Hepatology 58:337-50 (2013). PubMed: 23460364
      • Li J  et al. Anti-CCL25 antibody prolongs skin allograft survival by blocking CCR9 expression and impairing splenic T-cell function. Arch Immunol Ther Exp (Warsz) 61:237-44 (2013). PubMed: 23456208
      • Deutsch AJ  et al. Chemokine receptors in gastric MALT lymphoma: loss of CXCR4 and upregulation of CXCR7 is associated with progression to diffuse large B-cell lymphoma. Mod Pathol : (2012). IHC ; Human . PubMed: 22936065
      • Liu JX  et al. CCR7, CCR8, CCR9 and CCR10 in the mouse hippocampal CA1 area and the dentate gyrus during and after pilocarpine-induced status epilepticus. J Neurochem 100:1072-88 (2007). PubMed: 17181556
      • Marski M  et al. CD18 is required for intestinal T cell responses at multiple immune checkpoints. J Immunol 178:2104-12 (2007). Flow Cyt ; Mouse . PubMed: 17277114

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      For each of these antibodies, specifically what type of antigen retrieval was used for IHC-formalin fixed, paraffin embedded sections?

      Read More
      Answer

      Thank you for your patience. The originator of ab12953 and ab13396 has gotten back to me with the standard IHC protocol that they follow which I have included below. If you have any additional questions, please let me know. Tissue Preparation: Formalin fixation and embedding in paraffin wax Tissue Sectioning: Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C. Deparaffinization: Wash dry slides in 3 changes of xylene – 5 minutes each @ RT Rehydration: Wash slides in 3 changes of 100% alcohol – 3 minutes each @ RT Wash slides in 2 changes of 95% alcohol – 3 minutes each @ RT Wash slides in 1 change of 80% alcohol – 3 minutes @ RT Rinse slides in gentle running distilled water – 5 minutes @ RT Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute @ RT Immunostaining: (Do not allow tissues to dry at any time during the staining procedure) Apply a universal protein block – 20 minutes @ RT Drain protein block from slides, apply diluted primary antibody – 45 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply a biotinylated anti-rabbit IgG (H+L) secondary – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase streptavidin – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase chromogen substrate – 30 minutes @ RT Wash slides in distilled water – 1 minute @ RT Dehydrate: (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) Wash slides in 2 changes of 80% alcohol – 1 minute each @ RT Wash slides in 2 changes of 95% alcohol – 1 minute each @ RT Wash slides in 3 changes of 100% alcohol – 1 minute each @ RT Wash slides in 3 changes of xylene – 1 minute each @ RT Apply coverslip

      Read More

      Question

      For each of these antibodies, specifically what type of antigen retrieval was used for IHC-formalin fixed, paraffin embedded sections?

      Read More
      Answer

      Thank you for your phone call. For ab13268, ab1662, and ab1669, the originator has informed me that the antigen retrieval method for IHC-formalin fixed, paraffin embedded sections involved treating the sections with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. I will contact you again when I receive information regarding ab12953 and ab13396. Have a great weekend!

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com