Anti-CCR9 抗体 (ab1662)

Submit a review Q&A (2)References (6)

製品の概要

  • 製品名

    Anti-CCR9 antibody
    CCR9 一次抗体 製品一覧

  • 製品の詳細

    Goat polyclonal to CCR9

  • 由来種

    Goat

  • 特異性

    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region.

  • アプリケーション

    適用あり: IHC-FoFr, Flow Cyt, ELISA, IHC-Pmore details
    適用なし: WB

  • 種交差性

    交差種: Mouse
    交差が予測される動物種: Human

  • 免疫原

    Synthetic peptide:

    IPGMFDDFSYDSTASTDDYMNLNFSSFF

    , corresponding to amino acids 10-37 of Mouse CCR9.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • Mouse thymus or lymphnodes.

製品の特性

  • 製品の状態

    Liquid

  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

  • バッファー

    Preservative: 0.1% Sodium azide
    Constituent: 0.1% BSA
  • Concentration information loading...

  • 精製度

    Immunogen affinity purified

  • ポリ/モノ

    ポリクローナル

  • アイソタイプ

    IgG

  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab1662 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-FoFr 1/500 - 1/1000. After perfusion place in 30% sucrose overnight.
Flow Cyt 1/200.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
ELISA 1/100000.
IHC-P 1/250.
  • 追加情報
    Is unsuitable for WB.

    • ターゲット情報

    • 関連性

      The protein encoded by this gene is a member of the beta chemokine receptor family. It is predicted to be a seven transmembrane protein similar to G protein coupled receptors. Chemokines and their receptors are key regulators of the thymocytes migration and maturation in normal and inflammation conditions. This gene is expressed in a range of tissues and hemopoietic cells. The expression of this receptor in lymphatic endothelial cells and overexpression in vascular tumors suggested its function in chemokine-driven recirculation of leukocytes and possible chemokine effects on the development and growth of vascular tumors. This receptor appears to bind the majority of beta-chemokine family members; however, its specific function remains unknown. The specific ligand of this receptor is CCL25. It has been found that this gene is differentially expressed by T lymphocytes of small intestine and colon, suggested a role in the thymocytes recruitment and development that may permit functional specialization of immune responses in different segment of the gastrointestinal tract. This gene is mapped to chromosome 3p21.3, a region that includes a cluster of chemokine receptor genes. Two alternatively spliced transcript variants have been described.

    • 細胞内局在

      Cell membrane; Multi-pass membrane protein.

    • 参照データベース

      • 別名

        • C C chemokine receptor D6 antibody
        • C C chemokine receptor type 9 antibody
        • C C CKR 9 antibody
        • CC Chemokine receptor CCR10 antibody
        • CC CKR 9 antibody
        • CCBP2 antibody
        • CCCKR9 antibody
        • CCR 9 antibody
        • CCR9 antibody
        • CDw199 antibody
        • CDw199 antigen antibody
        • Chemokine (C C motif) receptor 9 antibody
        • Chemokine (C C motif) receptor 9, isoform CRA_a antibody
        • Chemokine (C-C motif) receptor 9 isoform A antibody
        • Chemokine (C-C motif) receptor 9 isoform B antibody
        • Chemokine C C motif receptor 9 antibody
        • Chemokine C C receptor 10 antibody
        • Chemokine CC Motif Receptor 9 antibody
        • Chemokine receptor CCR 9 antibody
        • Chemokine receptor CCR9 antibody
        • Chemokine-binding protein D6 antibody
        • Cmkbr10 antibody
        • CMKBR9 antibody
        • G protein coupled receptor 28 antibody
        • GPR 28 antibody
        • GPR 9 6 antibody
        • GPR28 antibody
        • GPR96 antibody
        • OTTHUMP00000164653 antibody
        • OTTHUMP00000164654 antibody
        see all

      画像

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody (ab1662)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody (ab1662)
        Immonuhistochemistry on mouse spleen, at 1/250 dilution.

      参考文献

      This product has been referenced in:

      • Huang Y  et al. Abrogation of CC chemokine receptor 9 ameliorates ventricular remodeling in mice after myocardial infarction. Sci Rep 6:32660 (2016). Read more (PubMed: 27585634) »
      • Chu PS  et al. C-C motif chemokine receptor 9 positive macrophages activate hepatic stellate cells and promote liver fibrosis in mice. Hepatology 58:337-50 (2013). Read more (PubMed: 23460364) »
      See all 6 Publications for this product

      ab1662 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

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      1-2 of 2 Abreviews or Q&A

      Question

      For each of these antibodies, specifically what type of antigen retrieval was used for IHC-formalin fixed, paraffin embedded sections?

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      Answer

      Thank you for your patience. The originator of ab12953 and ab13396 has gotten back to me with the standard IHC protocol that they follow which I have included below. If you have any additional questions, please let me know. Tissue Preparation: Formalin fixation and embedding in paraffin wax Tissue Sectioning: Make 4-µm sections and place on pre-cleaned and charged microscope slides. Heat in a tissue-drying oven for 45 minutes at 60°C. Deparaffinization: Wash dry slides in 3 changes of xylene – 5 minutes each @ RT Rehydration: Wash slides in 3 changes of 100% alcohol – 3 minutes each @ RT Wash slides in 2 changes of 95% alcohol – 3 minutes each @ RT Wash slides in 1 change of 80% alcohol – 3 minutes @ RT Rinse slides in gentle running distilled water – 5 minutes @ RT Antigen retrieval: Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes Remove from heat and let stand at room temperature in buffer - 20 minutes Rinse in 1X TBS with Tween (TBST) – 1 minute @ RT Immunostaining: (Do not allow tissues to dry at any time during the staining procedure) Apply a universal protein block – 20 minutes @ RT Drain protein block from slides, apply diluted primary antibody – 45 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply a biotinylated anti-rabbit IgG (H+L) secondary – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase streptavidin – 30 minutes @ RT Rinse slides in 1X TBST - 1 minute @ RT Apply alkaline phosphatase chromogen substrate – 30 minutes @ RT Wash slides in distilled water – 1 minute @ RT Dehydrate: (This method should only be used if the chromogen substrate is alcohol insoluble (e.g. Vector Red, DAB) Wash slides in 2 changes of 80% alcohol – 1 minute each @ RT Wash slides in 2 changes of 95% alcohol – 1 minute each @ RT Wash slides in 3 changes of 100% alcohol – 1 minute each @ RT Wash slides in 3 changes of xylene – 1 minute each @ RT Apply coverslip

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      Question

      For each of these antibodies, specifically what type of antigen retrieval was used for IHC-formalin fixed, paraffin embedded sections?

      Read More
      Answer

      Thank you for your phone call. For ab13268, ab1662, and ab1669, the originator has informed me that the antigen retrieval method for IHC-formalin fixed, paraffin embedded sections involved treating the sections with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. I will contact you again when I receive information regarding ab12953 and ab13396. Have a great weekend!

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