製品の概要

  • 製品名
    Anti-CCR7 antibody
    CCR7 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to CCR7
  • 由来種
    Goat
  • 特異性
    Peptide sequence is < 50 % identical to other mouse chemokine receptors in this region. The antibody has been tested in an academic lab on cells transfected with CCR7 receptor, we do not have information regarding its use against endogenous protein.
  • アプリケーション
    適用あり: ELISA, Flow Cyt, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide:

    DPGKPRKNVLVVALLVIFQVC

    , corresponding to amino acids 2-22 of Mouse CCR7.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • IHC-P: Mouse spleen, human spleen. WB: cells transfected with CCR7 receptor

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1657 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/100000.
Flow Cyt 1/10.

ab37373 - Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

 
IHC-P 1/250.
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

  • 機能
    Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
  • 組織特異性
    Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.
  • 配列類似性
    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
    • 別名
      • BLR 2 antibody
      • BLR2 antibody
      • C C chemokine receptor type 7 antibody
      • C C CKR 7 antibody
      • C-C chemokine receptor type 7 antibody
      • C-C CKR-7 antibody
      • CC chemokine receptor 7 antibody
      • CC chemokine receptor type 7 antibody
      • CC CKR 7 antibody
      • CC-CKR-7 antibody
      • CCCKR7 antibody
      • CCR 7 antibody
      • CCR-7 antibody
      • Ccr7 antibody
      • CCR7_HUMAN antibody
      • CD 197 antibody
      • CD197 antibody
      • CD197 antigen antibody
      • CDw197 antibody
      • Chemokine (C C motif) receptor 7 antibody
      • Chemokine (C C) receptor 7 antibody
      • Chemokine C C motif receptor 7 antibody
      • Chemokine C C receptor 7 antibody
      • Chemokine receptor 7 antibody
      • Chemokine receptor 7-like protein antibody
      • CMKBR7 antibody
      • EBI 1 antibody
      • EBI1 antibody
      • Ebi1h antibody
      • EBV Induced G Protein Coupled Receptor 1 antibody
      • EBV-induced G-protein coupled receptor 1 antibody
      • Epstein Barr virus induced G protein coupled receptor antibody
      • Epstein Barr virus induced gene 1 antibody
      • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
      • EVI 1 antibody
      • EVI1 antibody
      • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
      • MGC108519 antibody
      • MIP 3 beta receptor antibody
      • MIP-3 beta receptor antibody
      • MIP3 Beta Receptor antibody
      see all

    画像

    • Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody (ab1657)
      Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody (ab1657)

      Immunohistochemical staining for CCR7 in human spleen.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody (ab1657)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody (ab1657)
      ab1657, at a dilution of 1/250, staining CCR7 in mouse spleen section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    • Western blot - Anti-CCR7 antibody (ab1657)
      Western blot - Anti-CCR7 antibody (ab1657)
      Anti-CCR7 antibody (ab1657) at 1 µg/ml + K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate at 10 µg

      Secondary
      Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 43 kDa
      Observed band size: 43 kDa


      Exposure time: 20 minutes

    参考文献

    This product has been referenced in:
    • Salim SY  et al. CD83+CCR7- dendritic cells accumulate in the subepithelial dome and internalize translocated Escherichia coli HB101 in the Peyer's patches of ileal Crohn's disease. Am J Pathol 174:82-90 (2009). IHC-P ; Human . Read more (PubMed: 19095953) »
    • Pitkin L  et al. Expression of CC chemokine receptor 7 in tonsillar cancer predicts cervical nodal metastasis, systemic relapse and survival. Br J Cancer 97:670-7 (2007). IHC-P ; Human . Read more (PubMed: 17687340) »

    See all 8 Publications for this product

    ab1657 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    レビューと Q&A

    レビューを送る 質問を送る

    15 reviews or Q&A

    10
    Votes: Highest First

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR7 antibody

     Poor
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris-EDTA, pH9.0
    Sample
    Mouse Tissue sections (spleen)
    Specification
    spleen
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More
    abreview image
    Username

    Abcam user community

    Verified customer

    投稿 Nov 19 2014

    Immunohistochemistry (Frozen sections) abreview for Anti-CCR7 antibody

     Average
    Abreviews
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (spleen, lymph node)
    Specification
    spleen, lymph node
    Fixative
    Acetone
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More
    Username

    Mr. Jong Hyung Lim

    Verified customer

    投稿 Nov 12 2009

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR7 antibody

     Below Average
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tonsil)
    Specification
    tonsil
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    methanol/hydrogen peroxide (and I also tried 10% BSA) as blocking agent for 5 minute(s) · Concentration: 2.5%
    Read More
    Username

    Abcam user community

    Verified customer

    投稿 May 30 2007

    Huw called regarding the suggestions made.

    Thank you for your patience. I have been in touch with the source of this antibody once again and I have some additional information. Unfortunately at this time I cannot source an alternative lot. However, the originator mentioned the following val...

    Read More

    BATCH NUMBER 231100 ORDER NUMBER L076642 DESCRIPTION OF THE PROBLEM no staining on control sections (FFPE section of human tonsil and spleen) SAMPLE formalin fixed, paraffin embedded human tonsil tissue PRIMARY ANTIBODY abcam ccr7 ...

    Read More

    Thank you for taking the time to complete our questionaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody (ab16527). I have checked the procedure used to test this antibody in the laborat...

    Read More

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CCR7 antibody

     Poor
    Abreviews
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (spleen)
    Specification
    spleen
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 10%
    Read More
    Username

    Dr. Linda Svantesson

    Verified customer

    投稿 Nov 17 2006

    BATCH NUMBER 195970 ORDER NUMBER 163068 DESCRIPTION OF THE PROBLEM Non-specific staining, high background with no specific signal seen SAMPLE Human tonsil and mouse multi organ block (lung, liver, spleen) PRIMARY ANTIBODY Abc...

    Read More

    Thank you for your enquiry. I am sorry to hear that you have been obtaining non-specific staining using this antibody. I have read your technical questionnaire and I have a few comments. Please can you detail the duration of antigen retrieval t...

    Read More

    Obtaining actual WB band size of 60KDa, whereas the predicted is 43KDa (SwissProt)

    Thank you for your enquiry. Further to our telephone conversation yesterday afternoon I have been in touch with the source of this antibody. They informed me that by western blotting an actual molecular weight of 43KDa was detected in the following...

    Read More

    I have read your protocol and I don't know which is the difference between yours and mine. Please, Could you help me? Protocol: 1. Please describe the problem (high background, no staining etc). We have high background and no staining in li...

    Read More

    Thank you for your e-mail. We hope these answers are more helpful. 5. How did you block the unspecific binding sites? BSA 3% 30 minutes Instead, use 2% normal rabbit serum at room temperature to block the non-specific binding sites. 7. Seco...

    Read More

    1. Please describe the problem (high background, no staining etc). We have high background and no staining in limphocytes. 2. On what material are you testing the antibody in IHC? Human skin. Paraffin embedded 3. How did you fix the samples? ?...

    Read More

    We are very sorry you are having problems with your protocol. Here is the up to date detailed protocol: .

    1-10 of 15 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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