Anti-CCR7 抗体 [4B12] - BSA and Azide free (ab269572)

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Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)

    Key features and details

    • Rat monoclonal [4B12] to CCR7 - BSA and Azide free
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG2a

    製品の概要

    • 製品名

      Anti-CCR7 antibody [4B12] - BSA and Azide free
      CCR7 一次抗体 製品一覧

    • 製品の詳細

      Rat monoclonal [4B12] to CCR7 - BSA and Azide free

    • 由来種

      Rat

    • Tested Applications & Species

      Application Species
      Flow Cyt
      Mouse
      See all applications and species data

    • 免疫原

      Tissue, cells or virus. This information is proprietary to Abcam and/or its suppliers.

    • ポジティブ･コントロール

      • Flow Cyt: Mouse splenocytes.

    • 特記事項

      This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

      ab269572 is the carrier-free version of ab52602. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

       

      Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

      Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

      This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

      Maxpar® is a trademark of Fluidigm Canada Inc.

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab269572 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    アプリケーション Species
    Flow Cyt
    Mouse
    アプリケーション Abreviews 特記事項
    Flow Cyt
    Use a concentration of 5 - 10 µg/ml.

    ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. 
    特記事項
    Flow Cyt
    Use a concentration of 5 - 10 µg/ml.

    ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. 

    ターゲット情報

    • 機能

      Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.

    • 組織特異性

      Expressed in various lymphoid tissues and activated B- and T-lymphocytes, strongly up-regulated in B-cells infected with Epstein-Barr virus and T-cells infected with herpesvirus 6 or 7.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • BLR 2 antibody
        • BLR2 antibody
        • C C chemokine receptor type 7 antibody
        • C C CKR 7 antibody
        • C-C chemokine receptor type 7 antibody
        • C-C CKR-7 antibody
        • CC chemokine receptor 7 antibody
        • CC chemokine receptor type 7 antibody
        • CC CKR 7 antibody
        • CC-CKR-7 antibody
        • CCCKR7 antibody
        • CCR 7 antibody
        • CCR-7 antibody
        • Ccr7 antibody
        • CCR7_HUMAN antibody
        • CD 197 antibody
        • CD197 antibody
        • CD197 antigen antibody
        • CDw197 antibody
        • Chemokine (C C motif) receptor 7 antibody
        • Chemokine (C C) receptor 7 antibody
        • Chemokine C C motif receptor 7 antibody
        • Chemokine C C receptor 7 antibody
        • Chemokine receptor 7 antibody
        • Chemokine receptor 7-like protein antibody
        • CMKBR7 antibody
        • EBI 1 antibody
        • EBI1 antibody
        • Ebi1h antibody
        • EBV Induced G Protein Coupled Receptor 1 antibody
        • EBV-induced G-protein coupled receptor 1 antibody
        • Epstein Barr virus induced G protein coupled receptor antibody
        • Epstein Barr virus induced gene 1 antibody
        • Epstein-Barr virus-induced G-protein coupled receptor 1 antibody
        • EVI 1 antibody
        • EVI1 antibody
        • Lymphocyte Specific G Protein Coupled Peptide Receptor antibody
        • MGC108519 antibody
        • MIP 3 beta receptor antibody
        • MIP-3 beta receptor antibody
        • MIP3 Beta Receptor antibody
        see all

      画像

      • Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)
        Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)

        C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-CCR7 [4B12] antibody (ab52602) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).

        Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes

        This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab52602).

      プロトコール

      To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

      Click here to view the general protocols

      参考文献 (0)

      ab269572 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab269572 は論文での使用が確認できていません。

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