製品の概要

  • 製品名

  • 製品の詳細

    Goat polyclonal to CCR6
  • 由来種

    Goat
  • アプリケーション

    適用あり: IHC-Fr, ELISA, ICC, IHC-Pmore details
  • 種交差性

    交差種: Human
    非交差種: Mouse
  • 免疫原

    Synthetic peptide:

    YFVSVNTSYYSVDSEMLLCSLQEVRQFSR

    , corresponding to amino acids 18-46 of Human CCR6.

  • ポジティブ・コントロール

    • Human spleen or peripheral blood

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab1659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/250. PubMed: 19095953
ELISA 1/75000.
ICC 1/300.
IHC-P 1/250.

ターゲット情報

  • 機能

    Receptor for a C-C type chemokine. Binds to MIP-3-alpha/LARC and subsequently transduces a signal by increasing the intracellular calcium ions level.
  • 組織特異性

    Spleen, lymph nodes, appendix, and fetal liver. Expressed in lymphocytes, T-cells and B-cells but not in natural killer cells, monocytes or granulocytes.
  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family.
  • 細胞内局在

    Cell membrane.
  • Information by UniProt
  • 参照データベース

  • 別名

    • BN 1 antibody
    • BN1 antibody
    • C C chemokine receptor type 6 antibody
    • C C CKR 6 antibody
    • C-C chemokine receptor type 6 antibody
    • C-C CKR-6 antibody
    • CC chemokine receptor type 6 antibody
    • CC CKR 6 antibody
    • CC R6 antibody
    • CC-CKR-6 antibody
    • CCCKR6 antibody
    • CCR 6 antibody
    • CCR-6 antibody
    • Ccr6 antibody
    • CCR6_HUMAN antibody
    • CD 196 antibody
    • CD196 antibody
    • CD196 antigen antibody
    • Chemokine (C C motif) receptor 6 antibody
    • Chemokine (C C) receptor 6 antibody
    • Chemokine (CC motif) receptor 6 antibody
    • Chemokine (CC) receptor 6 antibody
    • Chemokine receptor 6 antibody
    • Chemokine receptor like 3 antibody
    • Chemokine receptor-like 3 antibody
    • CKR 6 antibody
    • CKR L3 antibody
    • CKR-L3 antibody
    • CKR6 antibody
    • CKRL3 antibody
    • CMKBR 6 antibody
    • CMKBR6 antibody
    • DCR 2 antibody
    • DCR2 antibody
    • DRY 6 antibody
    • DRY6 antibody
    • G protein coupled receptor 29 antibody
    • G-protein coupled receptor 29 antibody
    • GPR 29 antibody
    • GPR CY4 antibody
    • GPR-CY4 antibody
    • GPR29 antibody
    • GPRCY 4 antibody
    • GPRCY4 antibody
    • LARC receptor antibody
    • Seven transmembrane receptor lymphocyte 22 antibody
    • STRL 22 antibody
    • STRL22 antibody
    see all

画像

  • Immunohistochemistry on paraffin section of human spleen, at 1/250 dilution.

参考文献

This product has been referenced in:

  • Salim SY  et al. CD83+CCR7- dendritic cells accumulate in the subepithelial dome and internalize translocated Escherichia coli HB101 in the Peyer's patches of ileal Crohn's disease. Am J Pathol 174:82-90 (2009). IHC-Fr ; Human . Read more (PubMed: 19095953) »
See 1 Publication for this product

レビューと Q&A

1-3 of 3 Abreviews or Q&A

Question
Answer

I was informed that the best negative control is affinity purified goat IgG antibody to a protein that is not expressed on the target cells cells/tissues to be examined. For example affinity purified Goat IgG antibody to Trail-2 or Trail-3 receptor antibody will be a good negative control. In our studies these antibodies failed to stain lymphoid cells. Other similar antigen-affinity purified goat IgG antibodies can also be used as negative controls. I hope this information will be useful,

Read More

Question
Answer

Thank you for your enquiry. The molecular weight of this antibody is 425kDa. Please contact us again if you require further information.

Read More

Answer

The source of ab1659 has replied to me the following: "We agree with the customer that antibody to human CCR6 should not react with the mouse CCR6 because of the significant sequence differences. However, work with mouse white bloods cells showed clearly positive blot results. At the present time we are looking at that data with more scrutiny. From now on the datasheets should be modified recommending that the antibody should be sold only for human CCR6 receptor only. We know that the extracellular domain of CCR6 does not extend up to amino acid 46 but due to glycosylation sites at the amino terminus of the extracellular domain of human CCR6. We chose to include additional residues at the C-terminal end of the peptide due to scientific and technical reasons that are confidential." Following this correspondence we have decided to change our datasheet to reflect your findings and those of the originator. I would like to offer you our sincere apologies for this problem and arrange a refund to be sent to you. Could you please let me know the Abcam order number and/or PO number and name of the person who placed the order and I will arrange this immediately. I'm very sorry for the inconvenience and look forward to hearing from you,

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