保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
バッファーPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
Concentration information loading...
精製度Immunogen affinity purified
Immunizing Peptide (Blocking)
Our Abpromise guarantee covers the use of ab65850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).Can be blocked with CCR5 peptide (ab192862).|
機能Receptor for a number of inflammatory CC-chemokines including MIP-1-alpha, MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 R5 isolates.
組織特異性Highly expressed in spleen, thymus, in the myeloid cell line THP-1, in the promyeloblastic cell line KG-1A and on CD4+ and CD8+ T-cells. Medium levels in peripheral blood leukocytes and in small intestine. Low levels in ovary and lung.
関連疾患Genetic variation in CCR5 is associated with suseptibility to diabetes mellitus insulin-dependent type 22 (IDDM22) [MIM:612522]. A multifactorial disorder of glucose homeostasis that is characterized by susceptibility to ketoacidosis in the absence of insulin therapy. Clinical fetaures are polydipsia, polyphagia and polyuria which result from hyperglycemia-induced osmotic diuresis and secondary thirst. These derangements result in long-term complications that affect the eyes, kidneys, nerves, and blood vessels.
配列類似性Belongs to the G-protein coupled receptor 1 family.
翻訳後修飾Sulfated on at least 2 of the N-terminal tyrosines. Sulfation contributes to the efficiency of HIV-1 entry and is required for efficient binding of the chemokines, CCL3 and CCL4.
O-glycosylated, but not N-glycosylated. Ser-6 appears to be the major site. Also sialylated glycans present which contribute to chemokine binding. Thr-16 and Ser-17 may also be glycosylated and, if so, with small moieties such as a T-antigen.
Palmitoylation in the C-terminal is important for cell surface expression, and to a lesser extent, for HIV entry.
Phosphorylation on serine residues in the C-terminal is stimulated by binding CC chemokines especially by APO-RANTES.
- Information by UniProt
- AM4 7 antibody
- C C chemokine receptor type 5 antibody
- C C CKR 5 antibody
All lanes : Anti-CCR5 antibody (ab65850) at 0.5 µg/ml
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate.
Lane 2 : COLO 205 (Human colon adenocarcinoma cell line) cell lysate.
Predicted band size: 41 kDa
Anti-CCR5 antibody (ab65850) at 0.5 µg + Mouse Liver Tissue Lysate at 50 µg
Predicted band size: 41 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
This product has been referenced in:
- Tomas-Sanchez C et al. Prophylactic Chronic Zinc Administration Increases Neuroinflammation in a Hypoxia-Ischemia Model. J Immunol Res 2016:4039837 (2016). ELISA ; Rat . Read more (PubMed: 27635404) »
- Che LF et al. Downregulation of CCR5 inhibits the proliferation and invasion of cervical cancer cells and is regulated by microRNA-107. Exp Ther Med 11:503-509 (2016). WB ; Human . Read more (PubMed: 26893637) »