Anti-CCR4 抗体 [KW-0761 (Mogamulizumab)] - Rabbit IgG (Chimeric) (ab275980)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [KW-0761 (Mogamulizumab)] to CCR4 - Chimeric
- Suitable for: WB
- Reacts with: Human
製品の概要
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製品名
Anti-CCR4 antibody [KW-0761 (Mogamulizumab)] - Rabbit IgG (Chimeric)
CCR4 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [KW-0761 (Mogamulizumab)] to CCR4 - Chimeric -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Other Immunogen Type corresponding to Human CCR4.
Database link: P51679 -
ポジティブ・コントロール
- WB: Human erebellum and heart tissue lysates.
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特記事項
This rabbit monoclonal chimeric antibody was made using the variable domain sequences of the original Human IgG1 format, for improved compatibility with existing reagents, assays and techniques.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Proclin 300
Constituent: 99.98% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
KW-0761 (Mogamulizumab) -
アイソタイプ
IgG -
軽鎖の種類
kappa -
研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab275980の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 2 µg/ml.
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特記事項 |
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WB
Use a concentration of 2 µg/ml. |
ターゲット情報
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機能
High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival. -
組織特異性
Predominantly expressed in the thymus, in peripheral blood leukocytes, including T-cells, mostly CD4+ cells, and basophils, and in platelets; at lower levels, in the spleen and in monocytes. Detected also in macrophages, IL-2-activated natural killer cells and skin-homing memory T-cells, mostly the ones expressing the cutaneous lymphocyte antigen (CLA). Expressed in brain microvascular and coronary artery endothelial cells. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
In natural killer cells, CCL22 binding induces phosphorylation on yet undefined Ser/Thr residues, most probably by beta-adrenergic receptor kinases 1 and 2. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 1233 Human
- GenBank: NP_005499.1 Human
- Omim: 604836 Human
- SwissProt: P51679 Human
- Unigene: 184926 Human
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別名
- C C chemokine receptor type 4 antibody
- C C CKR 4 antibody
- C-C chemokine receptor type 4 antibody
see all
画像
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All lanes : Anti-CCR4 antibody [KW-0761 (Mogamulizumab)] - Rabbit IgG (Chimeric) (ab275980) at 2 µg/ml
Lane 1 : HDLM-2 cell lysate
Lane 2 : K562 cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Observed band size: 67 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-CCR4 antibody [KW-0761 (Mogamulizumab)] - Chimeric staining at 2 ug/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab275980 was shown to bind specifically to CCR4. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween$®$ 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L 800CW and Goat anti-Mouse IgG H&L 680RD at 1/20000 dilution.
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All lanes : Anti-CCR4 antibody [KW-0761 (Mogamulizumab)] - Rabbit IgG (Chimeric) (ab275980) at 2 µg/ml
Lane 1 : Human cerebellum tissue lysate
Lane 2 : Human heart tissue lysate
Lysates/proteins at 35 µg per lane.10% SDS PAGE gel
A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected running size for CCR4 is 37.7kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab275980 は論文での使用が確認できていません。