製品の概要

  • 製品名
    Anti-CCR3 antibody [Y31]
    CCR3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [Y31] to CCR3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC/IF or IP
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque monkey
  • 免疫原

    Synthetic peptide within Human CCR3 (C terminal). The exact sequence is proprietary.

  • ポジティブ・コントロール
    • K562 and Daudi cell lysates and human liver tissue.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab32512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500. Predicted molecular weight: 43 kDa.
IHC-P 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • ターゲット情報

    • 機能
      Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
    • 組織特異性
      In eosinophils as well as trace amounts in neutrophils and monocytes.
    • 配列類似性
      Belongs to the G-protein coupled receptor 1 family.
    • 細胞内局在
      Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • B chemokine receptor antibody
      • b-chemokine receptor antibody
      • C C chemokine receptor type 3 antibody
      • C C CKR 3 antibody
      • C-C chemokine receptor type 3 antibody
      • C-C CKR-3 antibody
      • CC chemokine receptor 3 antibody
      • CC chemokine receptor type 3 antibody
      • CC CKR 3 antibody
      • CC R3 antibody
      • CC-CKR-3 antibody
      • CCCKR3 antibody
      • CCR 3 antibody
      • CCR-3 antibody
      • CCR3 antibody
      • CCR3_HUMAN antibody
      • CD 193 antibody
      • CD193 antibody
      • CD193 antigen antibody
      • Chemokine (C C motif) receptor 3 antibody
      • chemokine (C-C motif) receptor 3 antibody
      • Chemokine (CC) receptor 1 like 2 antibody
      • Chemokine CC motif receptor 3 antibody
      • Chemokine receptor 3 antibody
      • CK R3 antibody
      • CKR 3 antibody
      • CKR3 antibody
      • CMKB R3 antibody
      • CMKBR 3 antibody
      • CMKBR1L2 antibody
      • CMKBR3 antibody
      • Eosinophil CC chemokine receptor 3 antibody
      • Eosinophil eotaxin receptor antibody
      • Eotaxin receptor antibody
      • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
      • MGC102841 antibody
      • MIP1 Alpha RL2 antibody
      see all

    画像

    • Immunohistochemical analysis of paraffin-embedded human liver tissue using ab32512 at 1/100 dilution.
    • All lanes : Anti-CCR3 antibody [Y31] (ab32512) at 1/500 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Daudi cell lysate

      Predicted band size: 43 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    参考文献

    This product has been referenced in:
    • Kuse Y  et al. CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure. Front Pharmacol 8:207 (2017). WB ; Mouse . Read more (PubMed: 28458639) »
    • Lee YS  et al. Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways. Oncotarget 7:36842-36853 (2016). Flow Cyt . Read more (PubMed: 27167205) »
    See all 12 Publications for this product

    レビューと Q&A

    1-4 of 4 Abreviews or Q&A

    Answer

    Thank you very much for your reply and for sending this additional information.

    Your credit note ID is ***.

    I am sorry that these antibodies did not perform as stated on the datasheet. If payment has already been made on the original order and you wish to receive a refund, please ask your purchasing department to contact our accounting department at mailto:us.credits@abcam.com so that we may gather the correct information needed for the refund. Please refer to the credit note ID in any correspondence with our accounting department.

    I hope this experience will not prevent you from purchasing other products from us in the future. Our Scientific Support team is always at your service, should you require furtherassistance or advice.

    Read More

    Question

    Inquiry: I am having trouble getting signal using your CCR2 (ab32144, lot GR52022-3), CCR3 (ab32512, lot GR52022-3), and CCR5 (ab32048, lot GR40445-5) antibodies. Here are my Western Blotting conditions: 1. Loaded 75, 50, 30, 20, and 10 micrograms per well of mouse spleen extract under both reducing and non-reducing conditions on a 4-12% Bis-tris gel from Invitrogen. MES buffer was used for the run. 2. Proteins were transferred onto a nitrocellulose membrane using an iBlot system (Invitrogen). 3. Membrane was blocked for 1 hour RT in 5% milk 4. Primary incubation for all three antibodies was overnight (approx. 16 hours) at 4 C. Primary antibodies were diluted 1:500 in 5% milk. 5. Secondary incubation was using goat anti-rabbit from santa cruz (1:5,000) for 1 hour RT. 6. Membrane was washed following both primary and secondary incubations three times in PBS-T. 7. West Pico was used to develop the blot, and bands were visualized using an ImageQuant LAS4000, and exposures were for 30 minutes. Our primary goal is to quantify changes in CCR2, 3, and 5 in brain and brain endothelial cells, however, to optimize antibody conditions, we typically use some other tissue type as a positive control. For CCRs, spleen seemed suitable. We see very a very faint band for CCR2 which appears to be at the correct molecular weight, but IR is very weak. We did not detect any bands for CCR5, and the bands observed for CCR3 were not the correct molecular weight. That all three antibodies did not work as expected is of concern to me. I'd appreciate your assistance with this issue.

    Read More
    Answer

    Thank you very much for contacting us and letting us know about the trouble with these antibodies.

    Thank you also for sending so many details of your protocol.The protocol seems to be ok and similar to the protocol that the lab uses to test this antibody, and I would expect to see bands at the correct molecular weights under these conditions. Does the anti-rabbit secondary antibody work with other primary antibodies? Was protein transfer confirmed with a Ponceau stain or some other method? If you probed for a loading control like actin, what were the results?

    We do fully guarantee these antibodies to work in Western blot with mouse samples, so if necessary I can send replacements or issue credits or refunds.

    I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.

    Read More

    Answer

    Thank you for your inquiry.

    In general we recommend to use EDTA pH9. This antigen retrieval method is optimal for the most antigens. Please see the attachment and this publication (Emoto et al., J Histochem Cytochem. 2005 Nov ;53 (11) : 1311-21.) for more information.

    ab28775 was used successfully by our customers without, with citrate buffer pH6 and with Tris pH10. Please see Abreviews on the datasheet.

    The image on the datasheetfor ab32512 was generated with citrate buffer pH6.

    The optimal protocol usually has to be determined by the end user individually.

    I hope this information is helpful and wish your customer good luck with their experiments.

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Jurkat cells)
    Loading amount
    100000 cells
    Specification
    Jurkat cells
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

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    Verified customer

    投稿 May 25 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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