製品の概要
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製品名Anti-CCR3 antibody
CCR3 一次抗体 製品一覧 -
製品の詳細Goat polyclonal to CCR3
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由来種Goat
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特異性Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
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アプリケーション適用あり: ICC, IHC-P, WBmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide: C-VETFGTTSYYDDVGLLCEKADTRALMA, corresponding to N terminal amino acids 8-34 of Human CCR3.
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ポジティブ･コントロール
- This anitbody gave a positive signal in human lung, ovary and thymus tissue lysates.
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特記事項
Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.1% Sodium azide
Constituent: 0.1% BSA -
Concentration information loading... -
精製度Immunogen affinity purified
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一次抗体 備考Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab1667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | 1/150. | |
| IHC-P | 1/150. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
ターゲット情報
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機能Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
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組織特異性In eosinophils as well as trace amounts in neutrophils and monocytes.
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配列類似性Belongs to the G-protein coupled receptor 1 family.
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細胞内局在Cell membrane.
- Information by UniProt
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参照データベース
- Entrez Gene: 1232 Human
- Omim: 601268 Human
- SwissProt: P51677 Human
- Unigene: 506190 Human
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別名
- B chemokine receptor antibody
- b-chemokine receptor antibody
- C C chemokine receptor type 3 antibody
see all
画像
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Immunocytochemistry - Anti-CCR3 antibody (ab1667)Immunocytochemistry on human peripheral blood cells (acetone fixed cells). -
Western blot - Anti-CCR3 antibody (ab1667)All lanes : Anti-CCR3 antibody (ab1667) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Rabbit polyclonal to Goat IgG - H&L - Pre-Adsorbed (HRP) (ab65486) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 53 kDa
Observed band size: 55 kDa (why is the actual band size different from the predicted?)
Additional bands at: 100 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
プロトコール
参考文献
This product has been referenced in:
- Hulmi JJ et al. Prevention of chemotherapy-induced cachexia by ACVR2B ligand blocking has different effects on heart and skeletal muscle. J Cachexia Sarcopenia Muscle 9:417-432 (2018). IHC-P ; Mouse . Read more (PubMed: 29230965) »
- Luo X et al. Choroidal pericytes promote subretinal fibrosis after experimental photocoagulation. Dis Model Mech 11:N/A (2018). Read more (PubMed: 29622551) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"