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    ccr3-antibody-ab1667.pdf

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Immunology Innate Immunity Chemokines Beta Chemokine Rec. (CCR)
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Anti-CCR3 抗体 (ab1667)

  • Datasheet
  • SDS
Submit a review Q&A (3)References (19)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Immunocytochemistry - Anti-CCR3 antibody (ab1667)

    Key features and details

    • Goat polyclonal to CCR3
    • Suitable for: ICC
    • Reacts with: Human
    • Isotype: IgG

    こちらの製品もご検討ください

    二次抗体
    Product image
    Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)

    関連製品

    製品の概要

    • 製品名

      Anti-CCR3 antibody
      CCR3 一次抗体 製品一覧
    • 製品の詳細

      Goat polyclonal to CCR3
    • 由来種

      Goat
    • 特異性

      Peptide sequence is < 50 % identical to other human chemokine receptors in this region.
    • アプリケーション

      適用あり: ICCmore details
    • 種交差性

      交差種: Human
    • 免疫原

      Synthetic peptide corresponding to Human CCR3 aa 7-34 (N terminal).
      Sequence:

      CVETFGTTSYYDDVGLLCEKADTRALMA


      Database link: P51677
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項


      Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • バッファー

      pH: 7.20
      Preservative: 0.1% Sodium azide
      Constituents: 0.1% BSA, 0.11% Dibasic monohydrogen potassium phosphate, 0.05% Monobasic dihydrogen potassium phosphate, 0.82% Sodium chloride
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • 一次抗体 備考

      Human CCR 3 is a receptor for CC chemokine eotaxin-1 and eotaxin-2. CCR 3 is constitutively expressed on eosinophils, small intestine and colon. The human CCR 3 protein contains 356 amino acids.
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Immunology
      • Innate Immunity
      • Chemokines
      • Beta Chemokine Rec. (CCR)
      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • GPCR
      • Immunology
      • Immune System Diseases
      • Antiviral Signaling
      • HIV-related

    関連製品

    • Compatible Secondaries

      • Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) (ab150129)
      • Donkey Anti-Goat IgG H&L (HRP) (ab205723)
    • Isotype control

      • Goat IgG, polyclonal - Isotype Control (ab37373)

    アプリケーション

    Our Abpromise guarantee covers the use of ab1667 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    ICC 1/150.

    ターゲット情報

    • 機能

      Receptor for a C-C type chemokine. Binds to eotaxin, eotaxin-3, MCP-3, MCP-4, RANTES and MIP-1 delta. Subsequently transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection.
    • 組織特異性

      In eosinophils as well as trace amounts in neutrophils and monocytes.
    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.
    • 細胞内局在

      Cell membrane.
    • Target information above from: UniProt accession P51677 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 1232 Human
      • Omim: 601268 Human
      • SwissProt: P51677 Human
      • Unigene: 506190 Human
      • 別名

        • B chemokine receptor antibody
        • b-chemokine receptor antibody
        • C C chemokine receptor type 3 antibody
        • C C CKR 3 antibody
        • C-C chemokine receptor type 3 antibody
        • C-C CKR-3 antibody
        • CC chemokine receptor 3 antibody
        • CC chemokine receptor type 3 antibody
        • CC CKR 3 antibody
        • CC R3 antibody
        • CC-CKR-3 antibody
        • CCCKR3 antibody
        • CCR 3 antibody
        • CCR-3 antibody
        • CCR3 antibody
        • CCR3_HUMAN antibody
        • CD 193 antibody
        • CD193 antibody
        • CD193 antigen antibody
        • Chemokine (C C motif) receptor 3 antibody
        • chemokine (C-C motif) receptor 3 antibody
        • Chemokine (CC) receptor 1 like 2 antibody
        • Chemokine CC motif receptor 3 antibody
        • Chemokine receptor 3 antibody
        • CK R3 antibody
        • CKR 3 antibody
        • CKR3 antibody
        • CMKB R3 antibody
        • CMKBR 3 antibody
        • CMKBR1L2 antibody
        • CMKBR3 antibody
        • Eosinophil CC chemokine receptor 3 antibody
        • Eosinophil eotaxin receptor antibody
        • Eotaxin receptor antibody
        • Macrophage inflammatory protein 1 alpha receptor like 2 antibody
        • MGC102841 antibody
        • MIP1 Alpha RL2 antibody
        see all

      画像

      • Immunocytochemistry - Anti-CCR3 antibody (ab1667)
        Immunocytochemistry - Anti-CCR3 antibody (ab1667)
        Immunocytochemistry on human peripheral blood cells (acetone fixed cells).

      プロトコール

      • Immunohistochemistry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
      • SDS
    • 参考文献 (19)

      ab1667 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab1667 は 19 報の論文で使用されています。

      • Venugopal N  et al. The primary cilium dampens proliferative signaling and represses a G2/M transcriptional network in quiescent myoblasts. BMC Mol Cell Biol 21:25 (2020). PubMed: 32293249
      • Jäger K  et al. Expression of Neural Crest Markers GLDC and ERRFI1 is Correlated with Melanoma Prognosis. Cancers (Basel) 11:N/A (2019). PubMed: 30641895
      • Saito Y  et al. Synbiotics suppress colitis-induced tumorigenesis in a colon-specific cancer mouse model. PLoS One 14:e0216393 (2019). PubMed: 31242213
      • Han Y  et al. IL-38 Ameliorates Skin Inflammation and Limits IL-17 Production from ?d T Cells. Cell Rep 27:835-846.e5 (2019). PubMed: 30995480
      • Shoemaker RH  et al. Evaluation of the STAT3 inhibitor GLG-302 for the prevention of estrogen receptor-positive and -negative mammary cancers. Oncol Rep 42:1205-1213 (2019). PubMed: 31322250
      View all Publications for this product

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-3 of 3 Abreviews or Q&A

      Question

      Thank you very much for your email and helpful suggestions. I think I will go ahead with ordering these antibodies and try to use one of your CCR3 antibodies for flow and see how I get on! I was wondering if you could send me a quote for these two antibodies? https://www.abcam.com/GPCR-GPR2-CCR10-antibody-ab3904.html https://www.abcam.com/CCR3-antibody-ab1667.html and I will just check with my supervisor that this is ok to order. Many thanks again for the help,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 09 2012

      Answer

      Thank you for your reply. A quotation has been sent to you in a separate email, the proforma reference number is : *****. DISCOUNT CODE for ab1667 to be tested in Flow Cytometry : ******** Expiration date: DD MM YYYY I am very pleased to hear you would like to accept our offer and test ab1667 in Flow Cytometry. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an Abreview forFlow Cytometryand include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jan 09 2012

      Question

      Dear Abcam, I am interested in two of your antibodies for chemokine receptors CCR3 and CCR10. I am looking for anti-human antibodies which I can use for both flow cytometry and for IHC-P. For CCR10 I have found the antibody ab3904 which seems to be suitable for these two applications. However for CCR3 I can find antibodies which are suitable either for flow cytometry OR for IHC-P - I was wondering if you had tested any of your antibodies for use for these two applications or whether you could advise me on which one might be suitable? eg ab1667 is only suggested for IHC-P, but do you think (or has anyone else ever used it) it could be used for flow? Or else could ab16231 be used for IHC-P as well as flow? Many thanks and look forward to hearing from you,

      Read More

      Abcam community

      Verified customer

      Asked on Jan 09 2012

      Answer

      Thank you very much for your interest in our anti-CCR3 antibodies. To our knowledge, none of ouranti-CCR3 antibodieshas not been tested in Flow Cytometry as well as in IHC-P. Therefore, I can offer a discount off a future purchase if you buy an anti-CCR3 now, test it in IHC-P or Flow Cytometryand submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. You couldbuy for example, ab16231 (https://www.abcam.com/ab16231)and test it in IHC-P, or an anti-CCR3 tested in IHC-P and test it in Flow Cytometry. The link to the list of thefour anti-CCR3 tested in IHC-P and humanis : https://www.abcam.com/index.html?pageconfig=searchresults&search=CCR3&pt=1&sk=app&sv=74&sn=IHC-P&l=3&fViewMore=1. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab***** in IHC-P or Flow Cytometry. I will then send a discount code. This code must be issued before purchasingthe antibody to be testedso please wait for my reply before ordering. 2. Purchase antibody to be tested either by phone, fax, or online (www.abcam.com). 3. Test it in IHC-P or Flow Cytometry. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even ifthe tested antibodyturns out to be unsuitable for IHC-P or Flow Cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount. Regarding the anti-CCR10, I confirm that ab3904 has been testedand is guaranteed to work in Flow Cytometry, IHC-P and on Human samples.

      Read More

      Abcam Scientific Support

      Answered on Jan 09 2012

      Question

      I have a customer who purchased your antibodies, ab1669 and ab1667. The customer would like to use them for immunohistochemistry of paraffin-embedded sections. Her sample is human lymphoid neoplasm. Please let me know an antigen retrieval method you recommend, respectively. And regarding each figure of IHC-formalin fixed, paraffin embedded sections please let me know also each antigen retrieval method when you got these data.

      Read More

      Abcam community

      Verified customer

      Asked on Feb 07 2007

      Answer

      Thank you for your enquiry. Ab1667: Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0) and steamed for 20 minutes followed by cooling for 20 minutes. Variations of the method will work successfully as shown in the following publication that has images as well: http://clincancerres.aacrjournals.org/cgi/content/full/11/7/2459 Ab1669: Antigen Retrieval: Sections were treated with antigen retrieval solution (Dako # S-1700, pH 6.0 or #S-3308, pH 8.0) and steamed for 20 minutes followed by cooling for 20 minutes. I hope that this information helps. Should you have any further questions then please do not hesitate to get back in touch with me.

      Read More

      Abcam Scientific Support

      Answered on Feb 08 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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