Anti-CCR2 抗体 [EPR22191] (ab227236)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22191] to CCR2
- Suitable for: Indirect ELISA, Flow Cyt
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-CCR2 antibody [EPR22191]
CCR2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR22191] to CCR2 -
由来種
Rabbit -
アプリケーション
適用あり: Indirect ELISA, Flow Cytmore details
適用なし: ICC/IF,IHC-P or WB -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt: Human peripheral blood mononuclear cell (PBMC).
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR22191 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab227236の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
Indirect ELISA |
Use at an assay dependent concentration.
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|
Flow Cyt |
1/500.
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特記事項 |
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Indirect ELISA
Use at an assay dependent concentration. |
Flow Cyt
1/500. |
ターゲット情報
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機能
Receptor for the MCP-1, MCP-3 and MCP-4 chemokines. Transduces a signal by increasing the intracellular calcium ions level. Alternative coreceptor with CD4 for HIV-1 infection. -
配列類似性
Belongs to the G-protein coupled receptor 1 family. -
翻訳後修飾
N-glycosylated. -
細胞内局在
Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 729230 Human
- Omim: 601267 Human
- SwissProt: P41597 Human
- Unigene: 511794 Human
- Unigene: 705362 Human
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別名
- C C chemokine receptor type 2 antibody
- C C CKR 2 antibody
- C-C chemokine receptor type 2 antibody
see all
画像
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Flow cytometry overlay histogram showing wild-type THP-1 (green line) and CCR2 knockout THP-1 cells (ab273716, red line) stained with ab227236. The cells were incubated in 1x PBS containing 10μg/ml human IgG and 10% normal goat serum to block non-specific protein-protein interaction followed by the antibody (ab227236) (1x106 in 100μl at 1 μg/ml) for 30 min at 4°C.
The secondary antibody Goat anti-rabbit IgG H&L (Alexa Fluor® 488, pre-adsorbed) (ab150081) was used at 1/2000 for 30 min at 4°C.
Isotype control antibody was Rabbit IgG (monoclonal) (ab172730) used at the same concentration and conditions as the primary antibody (wild-type THP-1 - black line; CCR2 THP-1 knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).
Acquisition of >5000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. -
ELISA analysis of Human CCR2 Protein recombinant protein at 1000 ng/ml with ab227236. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CCR2 with ab227236 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or CCR2 (Right). Data shown were gated on viable cells (PMID:10811011; PMID:22403612).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab227236 は論文での使用が確認できていません。