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Anti-CCR1 抗体 (ab19013)

Submit a review Q&A (2)References (5)
Western blot - Anti-CCR1 antibody (ab19013)

    Key features and details

    • Rabbit polyclonal to CCR1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-CCR1 antibody
      CCR1 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to CCR1

    • 由来種

      Rabbit

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human

    • 免疫原

      Synthetic peptide corresponding to Human CCR1. Surrounding amino acid 341.
      Database link: P32246
      (Peptide available as ab185720)

    • ポジティブ･コントロール

      • WB: Jurkat cell lysate.

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called 3414 CCR1 Antibody. 3414-100 is the same size as the 100 µg size of ab19013.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab19013の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 43 kDa.Can be blocked with CCR1 peptide (ab185720).
    特記事項
    WB
    Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 43 kDa.Can be blocked with CCR1 peptide (ab185720).

    ターゲット情報

    • 機能

      Receptor for a C-C type chemokine. Binds to MIP-1-alpha, MIP-1-delta, RANTES, and MCP-3 and, less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

    • 組織特異性

      Widely expressed in different hematopoietic cells.

    • 配列類似性

      Belongs to the G-protein coupled receptor 1 family.

    • 細胞内局在

      Cell membrane.
    • Information by UniProt

    • 参照データベース

      • 別名

        • C C chemokine receptor type 1 antibody
        • C C CKR 1 antibody
        • C-C chemokine receptor type 1 antibody
        • C-C CKR-1 antibody
        • CC CKR 1 antibody
        • CC-CKR-1 antibody
        • CCR 1 antibody
        • CCR-1 antibody
        • CCR1 antibody
        • CCR1_HUMAN antibody
        • CD 191 antibody
        • CD191 antibody
        • CD191 antigen antibody
        • Chemokine (C C motif) receptor 1 antibody
        • Chemokine C C motif receptor 1 antibody
        • CKR 1 antibody
        • CKR1 antibody
        • CMKBR 1 antibody
        • CMKBR1 antibody
        • CMKR 1 antibody
        • CMKR1 antibody
        • HM145 antibody
        • LD78 receptor antibody
        • Macrophage inflammatory protein 1 alpha /Rantes receptor antibody
        • Macrophage inflammatory protein 1 alpha receptor antibody
        • Macrophage inflammatory protein 1-alpha receptor antibody
        • Mip 1a R antibody
        • MIP 1alpha R antibody
        • MIP-1alpha-R antibody
        • MIP1aR antibody
        • RANTES R antibody
        • RANTES receptor antibody
        • RANTES-R antibody
        • SCYAR1 antibody
        see all

      画像

      • Western blot - Anti-CCR1 antibody (ab19013)
        Western blot - Anti-CCR1 antibody (ab19013)
        All lanes : Anti-CCR1 antibody (ab19013)

        All lanes : Jurkat cell lysate

        Predicted band size: 43 kDa

      参考文献 (5)

      ab19013 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab19013 は 5 報の論文で使用されています。

      • Liang Z  et al. Curcumin inhibits the migration of osteoclast precursors and osteoclastogenesis by repressing CCL3 production. BMC Complement Med Ther 20:234 (2020). PubMed: 32703287
      • Pincha N  et al. PAI1 mediates fibroblast-mast cell interactions in skin fibrosis. J Clin Invest 128:1807-1819 (2018). PubMed: 29584619
      • Zhang Y  et al. Effect of Tetramethylpyrazine on Atherosclerosis and SCAP/SREBP-1c Signaling Pathway in ApoE(-/-) Mice Fed with a High-Fat Diet. Evid Based Complement Alternat Med 2017:3121989 (2017). PubMed: 28491104
      • Hyldahl RD  et al. Satellite cell activity is differentially affected by contraction mode in human muscle following a work-matched bout of exercise. Front Physiol 5:485 (2014). IHC . PubMed: 25566087
      • Barron DA  et al. TGF-ß1 Induces an Age-Dependent Inflammation of Nerve Ganglia and Fibroplasia in the Prostate Gland Stroma of a Novel Transgenic Mouse. PLoS One 5:e13751 (2010). IHC . PubMed: 21060787

      レビューと Q&A

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      レビューを送る 質問を送る

      1-2 of 2 Abreviews or Q&A

      Question

      I have purchased the folllowing primary antibody: rabbit polyclonal to CCR1 (ab19013) I need as soon as possible to know if the antibody binds to the aminoterminal of the protein and, if it is possible, which is the specific sequence recognized by the antibody.

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      Answer

      Thank you for contacting us. Since the antibody was raised against the full length protein (Recombinant full length protein (Human): CCR1), and epitope mapping has not been performed - we do not have information about where the antibody binding site would be on the protein. This information is unfortunately unknown. I am very sorry to not be able to help in this case. Please do not hesitate to contact us if you need any more advice or information.

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      Question

      BATCH NUMBER 150039 DESCRIPTION OF THE PROBLEM more bands, high background, not clear bands unspecific binding, picture is attached ! SAMPLE A375 cell Lysat totalprotein PRIMARY ANTIBODY 2?g/?l over night room temperature DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED Cellysat from peritonealmacrophages ANTIBODY STORAGE CONDITIONS -20?C in Alliquots SAMPLE PREPARATION (TBS with1%Triton X-100)1ml + 5?l (200mM PMSF) AMOUNT OF PROTEIN LOADED 10,15,20,25?g Protein ELECTROPHORESIS/GEL CONDITIONS SDS-PAGE Gel TRANSFER AND BLOCKING CONDITIONS 2h transfer semi dry blotting 70mA Blocking 5% milk PBS, also tried with Tween 0,1% SECONDARY ANTIBODY Anti rabbit HPRT [a competitor] 1:2000 HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? Different blocking conditions , longer, with tween Antibody concentration higher dilution

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      Answer

      Thank you for contacting us for technical support and the protocol details, the image is very useful to understand the customer's problem. Is the customer using A357 cell lysate, not A375? This is a good positive control and ab19013 should work very well with it. The streaks on the lanes make me and my colleagues believe the problem is due to something gone off or missing in the migration process, it could be the gel, the buffers or the state of the samples too, especially as there are few protease inhibitors in the lysis buffer. Can I also confirm that the gel is reducing and the samples were reduced? What is the loading buffer recipe and gel recipe? Is there SDS in the running buffer? Finally, how long did the customer block the membrane for? Has the customer tried 5%BSA 1hr and incubating the antibody in TBST? If the customer would like our recommended WB tips and recipes please direct him/her to https://www.abcam.com/index.html?pageconfig=resource&rid=10413 I hope this information helps, please do not hesitate to contact me if I can be of further assistance,

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