Anti-CCL21 抗体 [MM0146-2H26] (ab89396)
Key features and details
- Mouse monoclonal [MM0146-2H26] to CCL21
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-CCL21 antibody [MM0146-2H26]
CCL21 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [MM0146-2H26] to CCL21 -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human CCL21.
Database link: O00585 -
ポジティブ・コントロール
- WB: Human placenta tissue lysate. IHC-P: Human placenta tissue.
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特記事項
Reconstituted antibody should be aliquoted and can be stored frozen at < -20 for at least six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute the antibody with 200 µl sterile PBS to give a final concentration of 0.5 mg/ml. -
保存方法
Shipped at 4°C. Store at -80°C. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and filtered through a 0.2 µm filter. -
ポリ/モノ
モノクローナル -
クローン名
MM0146-2H26 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89396の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Predicted molecular weight: 15 kDa.
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IHC-P |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/500 - 1/1000. Predicted molecular weight: 15 kDa. |
IHC-P
Use at an assay dependent concentration. |
ターゲット情報
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機能
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. -
組織特異性
Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart and fetal spleen. -
配列類似性
Belongs to the intercrine beta (chemokine CC) family. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 6366 Human
- Omim: 602737 Human
- SwissProt: O00585 Human
- Unigene: 57907 Human
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別名
- 6Ckine antibody
- Beta chemokine exodus 2 antibody
- Beta-chemokine exodus-2 antibody
see all
画像
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Anti-CCL21 antibody [MM0146-2H26] (ab89396) at 1/500 dilution + Human placenta tissue lysate
Predicted band size: 15 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted? -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4µm) labelling CCL21 with ab89396. Tissue was treated with 15µg/ml Protenase K for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab89396 は論文での使用が確認できていません。