保存方法Shipped at 4°C. Store at +4°C.
バッファーPreservative: 0.02% Sodium azide
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab62074 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 118 kDa.|
|IHC-P||Use a concentration of 5 µg/ml.|
機能Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.
組織特異性Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.
配列類似性Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain.
ドメインThe protein kinase domain is predicted to be catalytically inactive.
翻訳後修飾MMP14 cleaves PTK7 between Pro-621 and Leu-622 generating an N-terminal soluble (70 kDa) fragment and a membrane C-terminal (50 kDa) fragment. Proteolysis by MMP14 regulates PTK7 function in non-canonical Wnt signaling pathway.
細胞内局在Membrane. Cell junction. Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.
- Information by UniProt
- 8430404F20Rik antibody
- CCK 4 antibody
- CCK-4 antibody
All lanes : Anti-CCK4 antibody (ab62074) at 1 µg/ml
Lane 1 : Human colon tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : Rat colon tissue lysate
Lysates/proteins at 10 µg per lane.
All lanes : anti-rabbit IgG
Predicted band size: 118 kDa
ab62074 staining CCK4 in E9.5 mouse embryo tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 in PBS and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/500 in 5% donkey serum) for 12 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Image shows sagittal section of E9.5 mouse embryo. Neuroectoderm and underlying mesoderm labelled with ab62074 in green. DAPI stained nuclei in blue.
ab62074 at 5 µg/ml staining CCK4 in human colon tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections).
ab62074 has not yet been referenced specifically in any publications.