Anti-CBX2 抗体 (ab80044)
Key features and details
- Rabbit polyclonal to CBX2
- Suitable for: IP, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-CBX2 antibody
CBX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to CBX2 -
由来種
Rabbit -
特異性
From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help.
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アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Human CBX2 aa 500 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
(Peptide available asab91615) -
ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: JEG3 whole cell; HUVEC whole cell; HEK293 whole cell; MEL-1 whole cell; MEL-2 whole cell; HeLa nuclear; NIH3T3 whole cell; human testis tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab80044の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa).
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特記事項 |
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IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 52 kDa (predicted molecular weight: 56 kDa). |
ターゲット情報
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機能
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. Involved in sexual development, acting as activator of NR5A1 expression. -
関連疾患
Defects in CBX2 are the cause of 46,XY sex reversal type 5 (SRXY5) [MIM:613080]. It is a disorder of sex development. Affected individuals have a 46,XY karyotype but present as phenotypically normal females. -
配列類似性
Contains 1 A.T hook DNA-binding domain.
Contains 1 chromo domain. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 84733 Human
- Entrez Gene: 12416 Mouse
- Omim: 602770 Human
- SwissProt: Q14781 Human
- SwissProt: P30658 Mouse
- Unigene: 368410 Human
- Unigene: 14547 Mouse
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別名
- Cbx 2 antibody
- CBX2 antibody
- CBX2_HUMAN antibody
see all
画像
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All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/ml
Lane 1 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : MEL-1 (Human embryonic stem cell, male cell line) Whole Cell Lysate (ab27198)
Lane 5 : MEL-2 (Human embryonic stem cell, female cell line) Whole Cell Lysate (ab27196)
Lane 6 : Human testis tissue lysate - total protein (ab30257)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Additional bands at: 36 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
All lanes : Anti-CBX2 antibody (ab80044) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 56 kDa
Observed band size: 56 kDa
Exposure time: 8 minutes -
cbx2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to cbx2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab80044.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 52kDa: cbx2.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (12)
ab80044 は 12 報の論文で使用されています。
- Suh JL et al. Reprogramming CBX8-PRC1 function with a positive allosteric modulator. Cell Chem Biol 29:555-571.e11 (2022). PubMed: 34715055
- Zhou H et al. Expression and prognostic significance of CBX2 in colorectal cancer: database mining for CBX family members in malignancies and vitro analyses. Cancer Cell Int 21:402 (2021). PubMed: 34321009
- Sato T et al. Lin28a/let-7 pathway modulates the Hox code via Polycomb regulation during axial patterning in vertebrates. Elife 9:N/A (2020). PubMed: 32479258
- Dou Y et al. Effects of Wnt/ß-Catenin Signal Pathway Regulated by miR-342-5p Targeting CBX2 on Proliferation, Metastasis and Invasion of Ovarian Cancer Cells. Cancer Manag Res 12:3783-3794 (2020). PubMed: 32547214
- Zhou H et al. Elevated expression of minichromosome maintenance 3 indicates poor outcomes and promotes G1/S cell cycle progression, proliferation, migration and invasion in colorectal cancer. Biosci Rep 40:N/A (2020). PubMed: 32597491