Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8578] to CBS - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
製品名Anti-CBS antibody [EPR8578] - BSA and Azide free
CBS 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR8578] to CBS - BSA and Azide free
アプリケーション適用あり: WBmore details
適用なし: Flow Cyt,ICC,IHC-P or IP
Synthetic peptide within Human CBS aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: P35520
- WB: Raji and HeLa cell lysates.
ab248359 is the carrier-free version of ab131155 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab248359 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
解離定数（KD 値）KD = 6.78 x 10 -11 M Learn more about KD
Concentration information loading...
Immunizing Peptide (Blocking)
KO cell lines
KO cell lysates
Our Abpromise guarantee covers the use of ab248359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 61 kDa.Can be blocked with CBS peptide (ab183541).|
機能Only known pyridoxal phosphate-dependent enzyme that contains heme. Important regulator of hydrogen sulfide, especially in the brain, utilizing cysteine instead of serine to catalyze the formation of hydrogen sulfide. Hydrogen sulfide is a gastratransmitter with signaling and cytoprotective effects such as acting as a neuromodulator in the brain to protect neurons against hypoxic injury.
組織特異性In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
パスウェイAmino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-homocysteine and L-serine: step 1/2.
関連疾患Defects in CBS are the cause of cystathionine beta-synthase deficiency (CBSD) [MIM:236200]. CBSD is an enzymatic deficiency resulting in altered sulfur metabolism and homocystinuria. The clinical features of untreated homocystinuria due to CBS deficiency include myopia, ectopia lentis, mental retardation, skeletal anomalies resembling Marfan syndrome, and thromboembolic events. Light skin and hair can also be present. Biochemical features include increased urinary homocystine and methionine.
配列類似性Belongs to the cysteine synthase/cystathionine beta-synthase family.
Contains 1 CBS domain.
- Information by UniProt
- AI047524 antibody
- AI303044 antibody
- Beta thionase antibody
All lanes : Anti-CBS antibody [EPR8578] (ab131155) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : CBS knockout HeLa cell lysate
Lane 3 : Raji cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
This data was developed using the same antibody clone in a different buffer formulation (ab131155).
ab131155 Anti-CBS antibody [EPR8578] was shown to specifically react with CBS in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264950 (knockout cell lysate ab257203) was used. Wild-type and CBS knockout samples were subjected to SDS-PAGE. ab131155 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.