製品名CAY10626, PI3Kalpha/mTOR inhibitor
製品の詳細Potent PI3Kα/mTOR inhibitor
生理活性の詳細Potent, dual PI3Kα/mTOR inhibitor (IC50 values are 0.9 and 0.6 nM at PI3Kα and mTOR, respectively). Inhibits tumor cell growth, suppresses phosphorylation of downstream targets of PI3Kα and mTOR in vitro and promotes tumor regression in vivo.
保存方法Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
溶解性Soluble in DMSO to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120903 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab81283 staining AKT1 (phospho S473) in PC3 cells treated with CAY10626 (ab120903), by ICC/IF. Decrease of AKT1 (phospho S473) expression correlates with increased concentration of CAY10626, as described in literature.
The cells were incubated at 37°C for 24h in media containing different concentrations of ab120903 (CAY10626) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab81283 (1/100) dilution was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
- Chen Z et al. Synthesis and SAR of novel 4-morpholinopyrrolopyrimidine derivatives as potent phosphatidylinositol 3-kinase inhibitors. J Med Chem 53:3169-82 (2010). Read more (PubMed: 20334367) »
- Rameh LE & Cantley LC The role of phosphoinositide 3-kinase lipid products in cell function. J Biol Chem 274:8347-50 (1999). Read more (PubMed: 10085060) »