Anti-Caveolin-2 抗体 (ab2911)

Reviews (4)Q&A (3)References (11)
Western blot - Anti-Caveolin-2 antibody (ab2911)

    Key features and details

    • Rabbit polyclonal to Caveolin-2
    • Suitable for: WB
    • Reacts with: Rat
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-Caveolin-2 antibody
      Caveolin-2 一次抗体 製品一覧

    • 製品の詳細

      Rabbit polyclonal to Caveolin-2

    • 由来種

      Rabbit

    • 特異性

      Detects caveolin-2 from rat tissues. This antibody does not detect caveolin-1 or -3.

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Rat
      交差が予測される動物種: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant

    • 免疫原

      Synthetic peptide corresponding to Rat Caveolin-2 aa 1-19.
      Sequence:

      MGLETEKADVQLFMADDAY


      Database link: Q2IBC5
      (Peptide available as ab4929)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab2911の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB (2)
    Use at an assay dependent concentration. Can be blocked with Caveolin-2 peptide (ab4929).

    Can be blocked with Caveolin-2 peptide.
    特記事項
    WB
    Use at an assay dependent concentration. Can be blocked with Caveolin-2 peptide (ab4929).

    Can be blocked with Caveolin-2 peptide.

    ターゲット情報

    • 機能

      May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.

    • 組織特異性

      Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.

    • 配列類似性

      Belongs to the caveolin family.

    • 翻訳後修飾

      Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.

    • 細胞内局在

      Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
    • Information by UniProt

    • 参照データベース

      • 別名

        • CAV antibody
        • CAV2 antibody
        • CAV2_HUMAN antibody
        • Caveolae protein 20 kD antibody
        • Caveolin 2 antibody
        • Caveolin 2 isoform a and b antibody
        • Caveolin-2 antibody
        • MGC12294 antibody
        • OTTHUMP00000025032 antibody
        • OTTHUMP00000195982 antibody
        see all

      画像

      • Western blot - Anti-Caveolin-2 antibody (ab2911)
        Western blot - Anti-Caveolin-2 antibody (ab2911)

         Western blot of caveolin-2 on rat skeletal muscle protein extract using ab2911.

      参考文献 (11)

      ab2911 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2911 は 11 報の論文で使用されています。

      • Russell JS  et al. Cardiomyoblast caveolin expression: Effects of simulated diabetes, a-linolenic acid and cell signaling pathways. Am J Physiol Cell Physiol N/A:N/A (2020). PubMed: 32348174
      • Jiao F  et al. Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer. Cancer Cell Int 20:55 (2020). PubMed: 32099528
      • Yang X  et al. Twist1-induced miR-199a-3p promotes liver fibrosis by suppressing caveolin-2 and activating TGF-ß pathway. Signal Transduct Target Ther 5:75 (2020). PubMed: 32499481
      • Mortensen OV  et al. MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression. Front Cell Neurosci 11:253 (2017). PubMed: 28878626
      • Hoeke L  et al. Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2 regulation. PLoS One 8:e67300 (2013). WB . PubMed: 23826261
      View all Publications for this product

      レビューと Q&A

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      1-7 of 7 Abreviews or Q&A

      Immunocytochemistry abreview for Anti-Caveolin-2 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Mouse Cultured Cells (Cardiomyocytes)
      Permeabilization
      Yes - Triton x-100, 0.03%
      Specification
      Cardiomyocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 22 2018

      Immunocytochemistry abreview for Anti-Caveolin-2 antibody

       Good
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Rat Cultured Cells (Cardiomyocytes)
      Permeabilization
      Yes - Triton x-100, 0.03%
      Specification
      Cardiomyocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 16 2018

      Question

      Our customer purchased ab2910(lot#GR54402-1), ab2911(lot#GR11226-5) and would like to know KD values of these antibodies, respectively.
      https://www.abcam.com/Caveolin-1-antibody-Caveolae-Marker-ab2910.html
      https://www.abcam.com/Caveolin-2-antibody-Caveolae-Marker-ab2911.html
      Please let me know if you have information.

      Best regards

      Read More
      Answer

      Thank you for your enquiry.

      I am sorry to confirm that regrettablythe KD values for these two antibodies, ab2910 and ab2911, has not been assessed and so we are unable to provide this information. It is unusual for the KD values to be assessed for research grade antibodies.

      We aim to provide as much information as possible and I am sorry this is not possible on this occasion. If you have any further questions, please do not hesitate to contact me.

      Read More

      Question

      May I ask about expiry date of mentioned below antibodies? I couldn't find such info on your website (only storage instruction) and I'm not sure how long can I store them without the loss of their quality. Cat.no. Ab23841 Ab54481 Ab6276 Ab9484 Ab2920 Ab56023 Ab45966 Ab95453 Ab18199 Ab2911 Ab61249

      Read More
      Answer

      Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

      Read More

      Western blot abreview for Anti-Caveolin 2 antibody - Caveolae Marker

       Good
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Tissue lysate - whole (Colon)
      Loading amount
      20 µg
      Specification
      Colon
      Gel Running Conditions
      Reduced Denaturing (Trizingel)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 29 2011

      Western blot abreview for Anti-Caveolin 2 antibody - Caveolae Marker

       Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK 293, Kidney)
      Loading amount
      100 µg
      Specification
      HEK 293, Kidney
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
      Read More

      Dr. Olga Roche

      Verified customer

      投稿 Dec 19 2007

      Question

      Recently, I purchased rabbit caveolin-1 polyclonal (ab2910) and rabbit caveolin-2 polyclonal (ab2911). caveolin-1 worked beautifully using cell lysates derived from an immortalized human endothelial cell line. caveolin-2 was completely absent, which is a little surprising since the literature suggests that caveolin-1 and 2 are co-localized and highly expressed. What would you suggest as a positive control? Do you sell the immunizing peptide? I also noticed that the paper you reference for anti-caveolin-2 (JBC 272:29337-29346, 1997) did not use the polyclonal antibody you sell. Would you have the correct reference for this particular antibody?

      Read More
      Answer

      According to the datasheet, rat heart and skeletal muscle was used to test this antibody, so you can use it as positive control. We are sorry but we do not sell the immunizing peptide. We would suggest looking at the following reference, please take a look at it: Mol. Cell. Biol., 22 (11): 3905-3926, 2002. We will correct and update our datasheet with this new reference very shortly. Thank you for bringing this matter to our attention.

      Read More

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