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  1. Link

    caveolin-2-antibody-ab2911.pdf

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Tags & Cell Markers Subcellular Markers Organelles Caveolae and Clathrin
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Anti-Caveolin-2 抗体 (ab2911)

  • Datasheet
Reviews (4)Q&A (3)References (9)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Caveolin-2 antibody (ab2911)

    Key features and details

    • Rabbit polyclonal to Caveolin-2
    • Suitable for: WB
    • Reacts with: Mouse, Rat
    • Isotype: IgG

    こちらの製品もご検討ください

    タンパク質
    Product image
    Recombinant Human Caveolin-2 protein (ab114515)
    二次抗体
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

    関連製品

    製品の概要

    • 製品名

      Anti-Caveolin-2 antibody
      Caveolin-2 一次抗体 製品一覧
    • 製品の詳細

      Rabbit polyclonal to Caveolin-2
    • 由来種

      Rabbit
    • 特異性

      Detects caveolin-2 from rat tissues. This antibody does not detect caveolin-1 or -3.
    • アプリケーション

      適用あり: WBmore details
    • 種交差性

      交差種: Mouse, Rat
      交差が予測される動物種: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
    • 免疫原

      Synthetic peptide corresponding to Rat Caveolin-2 aa 1-19.
      Sequence:

      MGLETEKADVQLFMADDAY


      Database link: Q2IBC5
      (Peptide available as ab4929)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • 特記事項

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    製品の特性

    • 製品の状態

      Liquid
    • 保存方法

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • バッファー

      Preservative: 0.05% Sodium azide
      Constituents: 0.1% BSA, 99% PBS
    • Concentration information loading...
    • 精製度

      Immunogen affinity purified
    • ポリ/モノ

      ポリクローナル
    • アイソタイプ

      IgG
    • 研究分野

      • Tags & Cell Markers
      • Subcellular Markers
      • Organelles
      • Caveolae and Clathrin
      • Cell Biology
      • Cell Cycle
      • Cell Division
      • Other cell division antibodies
      • Cardiovascular
      • Atherosclerosis
      • Lipid transport
      • Cardiovascular
      • Atherosclerosis
      • Apoptosis

    関連製品

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Immunizing Peptide (Blocking)

      • Caveolin-2 peptide (ab4929)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Caveolin-2 protein (ab114515)

    アプリケーション

    Our Abpromise guarantee covers the use of ab2911 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    アプリケーション Abreviews 特記事項
    WB Use at an assay dependent concentration. Can be blocked with Caveolin-2 peptide (ab4929).

    Can be blocked with Caveolin-2 peptide.

    ターゲット情報

    • 機能

      May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
    • 組織特異性

      Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
    • 配列類似性

      Belongs to the caveolin family.
    • 翻訳後修飾

      Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
    • 細胞内局在

      Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
    • Target information above from: UniProt accession P51636 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 12390 Mouse
      • Entrez Gene: 100362824 Rat
      • Entrez Gene: 363425 Rat
      • SwissProt: Q9WVC3 Mouse
      • SwissProt: Q2IBC5 Rat
      • Unigene: 396075 Mouse
      • Unigene: 81070 Rat
      • 別名

        • CAV antibody
        • CAV2 antibody
        • CAV2_HUMAN antibody
        • Caveolae protein 20 kD antibody
        • Caveolin 2 antibody
        • Caveolin 2 isoform a and b antibody
        • Caveolin-2 antibody
        • MGC12294 antibody
        • OTTHUMP00000025032 antibody
        • OTTHUMP00000195982 antibody
        see all

      画像

      • Western blot - Anti-Caveolin-2 antibody (ab2911)
        Western blot - Anti-Caveolin-2 antibody (ab2911)

         Western blot of caveolin-2 on rat skeletal muscle protein extract using ab2911.

      プロトコール

      • Immunohistochemistry protocols
      • Western blot protocols

      Click here to view the general protocols

      データシートおよび資料

      • Datasheet
    • 参考文献 (9)

      ab2911 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      ab2911 は 9 報の論文で使用されています。

      • Russell JS  et al. Cardiomyoblast caveolin expression: Effects of simulated diabetes, a-linolenic acid and cell signaling pathways. Am J Physiol Cell Physiol N/A:N/A (2020). PubMed: 32348174
      • Mortensen OV  et al. MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression. Front Cell Neurosci 11:253 (2017). PubMed: 28878626
      • Hoeke L  et al. Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2 regulation. PLoS One 8:e67300 (2013). WB . PubMed: 23826261
      • Cho WJ  et al. Caveolin-1 exists and may function in cardiomyocytes. Can J Physiol Pharmacol 88:73-6 (2010). IHC-P ; Mouse . PubMed: 20130741
      • Källquist L  et al. Neutrophil elastase and proteinase 3 trafficking routes in myelomonocytic cells. Exp Cell Res 316:3182-96 (2010). PubMed: 20828556
      • Zaas DW  et al. Counteracting Signaling Activities in Lipid Rafts Associated with the Invasion of Lung Epithelial Cells by Pseudomonas aeruginosa. J Biol Chem 284:9955-64 (2009). WB ; Mouse . PubMed: 19211560
      • Cho WJ  et al. Matrix metalloproteinase-2, caveolins, focal adhesion kinase and c-Kit in cells of the mouse myocardium. J Cell Mol Med 11:1069-86 (2007). PubMed: 17979883
      • Shu L & Shayman JA Caveolin-associated accumulation of globotriaosylceramide in the vascular endothelium of alpha-galactosidase A null mice. J Biol Chem 282:20960-7 (2007). WB ; Mouse . PubMed: 17535804
      • Zaas DW  et al. Pseudomonas invasion of type I pneumocytes is dependent on the expression and phosphorylation of caveolin-2. J Biol Chem 280:4864-72 (2005). PubMed: 15545264

      レビューと Q&A

      Show All レビュー Q&A
      レビューを送る 質問を送る

      1-7 of 7 Abreviews or Q&A

      Immunocytochemistry abreview for Anti-Caveolin-2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Mouse Cultured Cells (Cardiomyocytes)
      Permeabilization
      Yes - Triton x-100, 0.03%
      Specification
      Cardiomyocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 22 2018

      Immunocytochemistry abreview for Anti-Caveolin-2 antibody

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunocytochemistry
      Sample
      Rat Cultured Cells (Cardiomyocytes)
      Permeabilization
      Yes - Triton x-100, 0.03%
      Specification
      Cardiomyocytes
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
      Fixative
      Paraformaldehyde
      Read More

      Abcam user community

      Verified customer

      投稿 Feb 16 2018

      Question

      Our customer purchased ab2910(lot#GR54402-1), ab2911(lot#GR11226-5) and would like to know KD values of these antibodies, respectively.
      https://www.abcam.com/Caveolin-1-antibody-Caveolae-Marker-ab2910.html
      https://www.abcam.com/Caveolin-2-antibody-Caveolae-Marker-ab2911.html
      Please let me know if you have information.

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on May 15 2012

      Answer

      Thank you for your enquiry.

      I am sorry to confirm that regrettablythe KD values for these two antibodies, ab2910 and ab2911, has not been assessed and so we are unable to provide this information. It is unusual for the KD values to be assessed for research grade antibodies.

      We aim to provide as much information as possible and I am sorry this is not possible on this occasion. If you have any further questions, please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on May 15 2012

      Question

      May I ask about expiry date of mentioned below antibodies? I couldn't find such info on your website (only storage instruction) and I'm not sure how long can I store them without the loss of their quality. Cat.no. Ab23841 Ab54481 Ab6276 Ab9484 Ab2920 Ab56023 Ab45966 Ab95453 Ab18199 Ab2911 Ab61249

      Read More

      Abcam community

      Verified customer

      Asked on Dec 27 2011

      Answer

      Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

      Read More

      Abcam Scientific Support

      Answered on Dec 27 2011

      Western blot abreview for Anti-Caveolin 2 antibody - Caveolae Marker

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Tissue lysate - whole (Colon)
      Loading amount
      20 µg
      Specification
      Colon
      Gel Running Conditions
      Reduced Denaturing (Trizingel)
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
      Read More

      Abcam user community

      Verified customer

      投稿 Sep 29 2011

      Western blot abreview for Anti-Caveolin 2 antibody - Caveolae Marker

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HEK 293, Kidney)
      Loading amount
      100 µg
      Specification
      HEK 293, Kidney
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
      Read More

      Dr. Olga Roche

      Verified customer

      投稿 Dec 19 2007

      Question

      Recently, I purchased rabbit caveolin-1 polyclonal (ab2910) and rabbit caveolin-2 polyclonal (ab2911). caveolin-1 worked beautifully using cell lysates derived from an immortalized human endothelial cell line. caveolin-2 was completely absent, which is a little surprising since the literature suggests that caveolin-1 and 2 are co-localized and highly expressed. What would you suggest as a positive control? Do you sell the immunizing peptide? I also noticed that the paper you reference for anti-caveolin-2 (JBC 272:29337-29346, 1997) did not use the polyclonal antibody you sell. Would you have the correct reference for this particular antibody?

      Read More

      Abcam community

      Verified customer

      Asked on Oct 13 2003

      Answer

      According to the datasheet, rat heart and skeletal muscle was used to test this antibody, so you can use it as positive control. We are sorry but we do not sell the immunizing peptide. We would suggest looking at the following reference, please take a look at it: Mol. Cell. Biol., 22 (11): 3905-3926, 2002. We will correct and update our datasheet with this new reference very shortly. Thank you for bringing this matter to our attention.

      Read More

      Asdf Edo

      Abcam Scientific Support

      Answered on Oct 13 2003

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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