アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    caveolin-2-antibody-ab2911.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Tags & Cell Markers Subcellular Markers Organelles Caveolae and Clathrin
Share by email

Anti-Caveolin-2 抗体 (ab2911)

  • Datasheet
Reviews (4)Q&A (3)References (11)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Caveolin-2 antibody (ab2911)
  • Western blot - Anti-Caveolin-2 antibody (ab2911)

Key features and details

  • Rabbit polyclonal to Caveolin-2
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Caveolin-2 antibody [EPR5471] (ab133484)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Caveolin-2 antibody
    Caveolin-2 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Caveolin-2
  • 由来種

    Rabbit
  • 特異性

    Detects caveolin-2 from rat tissues. This antibody does not detect caveolin-1 or -3.
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Sheep, Rabbit, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
  • 免疫原

    Synthetic peptide corresponding to Rat Caveolin-2 aa 1-100.
    Database link: Q2IBC5

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Cardiovascular
    • Atherosclerosis
    • Lipid transport
    • Cardiovascular
    • Atherosclerosis
    • Apoptosis

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Caveolin-2 protein (ab114515)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab2911の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (2)
Use at an assay dependent concentration.

Can be blocked with Caveolin-2 peptide.

特記事項
WB
Use at an assay dependent concentration.

Can be blocked with Caveolin-2 peptide.

ターゲット情報

  • 機能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity. Acts as an accessory protein in conjunction with CAV1 in targeting to lipid rafts and driving caveolae formation. The Ser-36 phosphorylated form has a role in modulating mitosis in endothelial cells. Positive regulator of cellular mitogenesis of the MAPK signaling pathway. Required for the insulin-stimulated nuclear translocation and activation of MAPK1 and STAT3, and the subsequent regulation of cell cycle progression.
  • 組織特異性

    Expressed in endothelial cells, smooth muscle cells, skeletal myoblasts and fibroblasts.
  • 配列類似性

    Belongs to the caveolin family.
  • 翻訳後修飾

    Phosphorylated on serine and tyrosine residues. CAV1 promotes phosphorylation on Ser-23 which then targets the complex to the plasma membrane, lipid rafts and caveolae. Phosphorylation on Ser-36 appears to modulate mitosis in endothelial cells (By similarity). Phosphorylation on both Tyr-19 and Tyr-27 is required for insulin-induced 'Ser-727' phosphorylation of STAT3 and its activation. Phosphorylation on Tyr-19 is required for insulin-induced phosphorylation of MAPK1 and DNA binding of STAT3. Tyrosine phosphorylation is induced by both EGF and insulin.
  • 細胞内局在

    Nucleus. Cytoplasm. Golgi apparatus membrane. Cell membrane. Membrane > caveola. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. Tyr-19-phosphorylated form is enriched at sites of cell-cell contact and is translocated to the nucleus in complex with MAPK1 in response to insulin (By similarity). Tyr-27-phosphorylated form is located both in the cytoplasm and plasma membrane. CAV1-mediated Ser-23-phosphorylated form locates to the plasma membrane. Ser-36-phosphorylated form resides in intracellular compartments.
  • Target information above from: UniProt accession P51636 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 858 Human
    • Entrez Gene: 12390 Mouse
    • Entrez Gene: 100362824 Rat
    • Entrez Gene: 363425 Rat
    • Omim: 601048 Human
    • SwissProt: P51636 Human
    • SwissProt: Q9WVC3 Mouse
    • SwissProt: Q2IBC5 Rat
    • Unigene: 212332 Human
    • Unigene: 603096 Human
    • Unigene: 396075 Mouse
    • Unigene: 81070 Rat
    see all
  • 別名

    • CAV antibody
    • CAV2 antibody
    • CAV2_HUMAN antibody
    • Caveolae protein 20 kD antibody
    • Caveolin 2 antibody
    • Caveolin 2 isoform a and b antibody
    • Caveolin-2 antibody
    • MGC12294 antibody
    • OTTHUMP00000025032 antibody
    • OTTHUMP00000195982 antibody
    see all

画像

  • Western blot - Anti-Caveolin-2 antibody (ab2911)
    Western blot - Anti-Caveolin-2 antibody (ab2911)

     Western blot of caveolin-2 on rat skeletal muscle protein extract using ab2911.

  • Western blot - Anti-Caveolin-2 antibody (ab2911)
    Western blot - Anti-Caveolin-2 antibody (ab2911)
    All lanes : Anti-Caveolin-2 antibody (ab2911) at 2 µg/ml

    Lane 1 : 3T3-L1 lysate
    Lane 2 : 3T3-L1 differentiated to adipocytes
    Lane 3 : Mouse Heart lysate
    Lane 4 : Mouse Lung lysate
    Lane 5 : Mouse Adipose lysate
    Lane 6 : Mouse Spleen lysate

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal Recombinant Secondary Antibody, HRP at 1/4000 dilution

プロトコール

  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • Datasheet download

    Download

参考文献 (11)

ab2911 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab2911 は 11 報の論文で使用されています。

  • Russell JS  et al. Cardiomyoblast caveolin expression: Effects of simulated diabetes, a-linolenic acid and cell signaling pathways. Am J Physiol Cell Physiol N/A:N/A (2020). PubMed: 32348174
  • Jiao F  et al. Caveolin-2 is regulated by BRD4 and contributes to cell growth in pancreatic cancer. Cancer Cell Int 20:55 (2020). PubMed: 32099528
  • Yang X  et al. Twist1-induced miR-199a-3p promotes liver fibrosis by suppressing caveolin-2 and activating TGF-ß pathway. Signal Transduct Target Ther 5:75 (2020). PubMed: 32499481
  • Mortensen OV  et al. MAP Kinase Phosphatase 3 (MKP3) Preserves Norepinephrine Transporter Activity by Modulating ERK1/2 Kinase-Mediated Gene Expression. Front Cell Neurosci 11:253 (2017). PubMed: 28878626
  • Hoeke L  et al. Intestinal Salmonella typhimurium infection leads to miR-29a induced caveolin 2 regulation. PLoS One 8:e67300 (2013). WB . PubMed: 23826261
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-7 of 7 Abreviews or Q&A

Immunocytochemistry abreview for Anti-Caveolin-2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Mouse Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 22 2018

Immunocytochemistry abreview for Anti-Caveolin-2 antibody

Good
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry
Sample
Rat Cultured Cells (Cardiomyocytes)
Permeabilization
Yes - Triton x-100, 0.03%
Specification
Cardiomyocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Feb 16 2018

Western blot abreview for Anti-Caveolin 2 antibody - Caveolae Marker

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Pig Tissue lysate - whole (Colon)
Loading amount
20 µg
Specification
Colon
Gel Running Conditions
Reduced Denaturing (Trizingel)
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2%
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Sep 29 2011

Western blot abreview for Anti-Caveolin 2 antibody - Caveolae Marker

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK 293, Kidney)
Loading amount
100 µg
Specification
HEK 293, Kidney
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Olga Roche

Verified customer

投稿 Dec 19 2007

Question

Our customer purchased ab2910(lot#GR54402-1), ab2911(lot#GR11226-5) and would like to know KD values of these antibodies, respectively.
https://www.abcam.com/Caveolin-1-antibody-Caveolae-Marker-ab2910.html
https://www.abcam.com/Caveolin-2-antibody-Caveolae-Marker-ab2911.html
Please let me know if you have information.

Best regards

Read More

Abcam community

Verified customer

Asked on May 15 2012

Answer

Thank you for your enquiry.

I am sorry to confirm that regrettablythe KD values for these two antibodies, ab2910 and ab2911, has not been assessed and so we are unable to provide this information. It is unusual for the KD values to be assessed for research grade antibodies.

We aim to provide as much information as possible and I am sorry this is not possible on this occasion. If you have any further questions, please do not hesitate to contact me.

Read More

Abcam Scientific Support

Answered on May 15 2012

Question

May I ask about expiry date of mentioned below antibodies? I couldn't find such info on your website (only storage instruction) and I'm not sure how long can I store them without the loss of their quality. Cat.no. Ab23841 Ab54481 Ab6276 Ab9484 Ab2920 Ab56023 Ab45966 Ab95453 Ab18199 Ab2911 Ab61249

Read More

Abcam community

Verified customer

Asked on Dec 27 2011

Answer

Thank you for your inquiry. We do not assign strict expiration dates for our products, however we follow general guidelines: Antibodies stored at 4C may be viable up to 1 year, those stored at -20C may be viable for up to 5 years, and those stored at -80C may be viable for up to 10 years. I hope this information helps. Please contact us with any other questions.

Read More

Abcam Scientific Support

Answered on Dec 27 2011

Question

Recently, I purchased rabbit caveolin-1 polyclonal (ab2910) and rabbit caveolin-2 polyclonal (ab2911). caveolin-1 worked beautifully using cell lysates derived from an immortalized human endothelial cell line. caveolin-2 was completely absent, which is a little surprising since the literature suggests that caveolin-1 and 2 are co-localized and highly expressed. What would you suggest as a positive control? Do you sell the immunizing peptide? I also noticed that the paper you reference for anti-caveolin-2 (JBC 272:29337-29346, 1997) did not use the polyclonal antibody you sell. Would you have the correct reference for this particular antibody?

Read More

Abcam community

Verified customer

Asked on Oct 13 2003

Answer

According to the datasheet, rat heart and skeletal muscle was used to test this antibody, so you can use it as positive control. We are sorry but we do not sell the immunizing peptide. We would suggest looking at the following reference, please take a look at it: Mol. Cell. Biol., 22 (11): 3905-3926, 2002. We will correct and update our datasheet with this new reference very shortly. Thank you for bringing this matter to our attention.

Read More

Asdf Edo

Abcam Scientific Support

Answered on Oct 13 2003

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

技術情報や製品情報をメールで受け取る 登録
製品・研究分野別
  • 癌
  • 循環器
  • 細胞生物学
  • 発生
  • エピジェネティクス&核内情報
  • 免疫学
  • メタボリズム
  • 感染症
  • ニューロサイエンス
  • シグナル伝達
  • ステムセル
製品・タイプ別
  • 一次抗体
  • 二次抗体
  • バイオケミカルズ
  • アイソタイプ・コントロール
  • マルチカラー・セレクター
  • キット
  • ローディング・コントロール
  • ライゼート
  • ペプチド
  • タンパク質
  • スライド
  • タグ抗体・細胞マーカー
  • その他試薬
サービス&サポート
  • サポート
  • よくあるお問い合わせ
  • プロトコール&トラブルシュート
  • ご購入に関して
  • お問い合わせフォーム
  • トレーニング
  • ターゲット別に見る
アブカムについて
  • 企業ウェブサイト
  • 投資家の皆様へ
  • 企業情報
  • 採用情報
  • アブカムについて
イベント
  • 展示会
  • カンファレンス
International websites
  • abcam.com
  • abcam.cn

Join with us

  • LinkedIn
  • facebook
  • Twitter
  • YouTube
  • Terms of sale
  • Website terms of use
  • Cookie policy
  • Privacy policy
  • Legal
© 1998-2022 Abcam plc. All rights reserved.