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    caveolin-1-antibody-caveolae-marker-ab2910.pdf

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遺伝子ノックアウト細胞株 検証済

Anti-Caveolin-1 抗体 - Caveolae Marker (ab2910)

  • Datasheet
  • SDS
Reviews (55)Q&A (10)References (122)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

Key features and details

  • Rabbit polyclonal to Caveolin-1 - Caveolae Marker
  • Suitable for: ICC, WB
  • Knockout validated
  • Reacts with: Rat, Human
  • Isotype: IgG

こちらの製品もご検討ください

タンパク質
Product image
Recombinant Human Caveolin-1 protein (ab114170)
Biochemical
Simvastatin, HMG-CoA reductase inhibitor (ab120505)
二次抗体
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

関連製品

製品の概要

  • 製品名

    Anti-Caveolin-1 antibody - Caveolae Marker
    Caveolin-1 一次抗体 製品一覧
  • 製品の詳細

    Rabbit polyclonal to Caveolin-1 - Caveolae Marker
  • 由来種

    Rabbit
  • 特異性

    Detects caveolin-1.
  • Tested Applications & Species

    Application Species
    ICC
    Rat
    Human
    WB
    Rat
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide corresponding to Human Caveolin-1 aa 1-17.
    Sequence:

    MSGGKYVDSEGHLYTVP


    Database link: Q03135
    (Peptide available as ab4928)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ・コントロール

    • WB: Human lung, heart and spleen tissue lysates; Rat heart protein extract. ICC: HeLa and NIH/3T3 cells; Rat astrocytes.
  • 特記事項

    This antibody can be used as a marker for lipid raft fractions.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, PBS
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    ポリクローナル
  • アイソタイプ

    IgG
  • 研究分野

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Coat Proteins
    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Caveolae and Clathrin
    • Cancer
    • Tumor biomarkers
    • Other
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

関連製品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (ab150080)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Immunizing Peptide (Blocking)

    • Human Caveolin-1 peptide (ab4928)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Recombinant Protein

    • Recombinant Human Caveolin-1 protein (ab114170)
  • Related Products

    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)

アプリケーション

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab2910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

アプリケーション Species
ICC
Rat
Human
WB
Rat
Human
All applications
Sheep
Rabbit
Horse
Cow
Cat
Pig
Chimpanzee
Gorilla
African green monkey
African bush elephant
アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
WB (22)
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).
特記事項
ICC
Use at an assay dependent concentration.
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).

ターゲット情報

  • 機能

    May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway.
  • 組織特異性

    Expressed in muscle and lung, less so in liver, brain and kidney.
  • 関連疾患

    Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes.
  • 配列類似性

    Belongs to the caveolin family.
  • 翻訳後修飾

    The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated.
  • 細胞内局在

    Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae.
  • Target information above from: UniProt accession Q03135 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 493668 Cat
    • Entrez Gene: 463670 Chimpanzee
    • Entrez Gene: 281040 Cow
    • Entrez Gene: 101125469 Gorilla
    • Entrez Gene: 857 Human
    • Entrez Gene: 404693 Pig
    • Entrez Gene: 100008837 Rabbit
    • Entrez Gene: 25404 Rat
    • Entrez Gene: 445468 Sheep
    • Omim: 601047 Human
    • SwissProt: A0M8S7 Cat
    • SwissProt: Q2QLF2 Chimpanzee
    • SwissProt: P79132 Cow
    • SwissProt: Q2IBF0 Gorilla
    • SwissProt: Q2QLB0 Horse
    • SwissProt: Q03135 Human
    • SwissProt: Q6RVA9 Pig
    • SwissProt: Q09YN6 Rabbit
    • SwissProt: P41350 Rat
    • SwissProt: Q6B3Y2 Sheep
    • Unigene: 74034 Human
    • Unigene: 22518 Rat
    see all
  • 別名

    • BSCL3 antibody
    • CAV antibody
    • CAV1 antibody
    • CAV1_HUMAN antibody
    • caveolae protein, 22 kD antibody
    • caveolin 1 alpha isoform antibody
    • caveolin 1 beta isoform antibody
    • Caveolin 1 caveolae protein 22kDa antibody
    • Caveolin-1 antibody
    • Caveolin1 antibody
    • cell growth-inhibiting protein 32 antibody
    • CGL3 antibody
    • LCCNS antibody
    • MSTP085 antibody
    • OTTHUMP00000025031 antibody
    • PPH3 antibody
    • VIP 21 antibody
    • VIP21 antibody
    see all

画像

  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml

    Lane 1 : Human lung
    Lane 2 : Human heart
    Lane 3 : Human spleen

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution

    Predicted band size: 20 kDa
    Observed band size: 20 kDa

  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    ab2910 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2910 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
    Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).

  • Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 2 µg/ml + Rat heart protein extract

    Predicted band size: 20 kDa

  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)

    Rat astrocytes stained with fluorescently labeled Caveolin-1 antibody.

    Primary antibody is ab2910 at a dilution of 1/500 and the secondary antibody is Texas red labeled anti-rabbit IgG at a dilution of 1/1000.

    This image was kindly supplied as part of the review submitted by Donghui Zhu.

  • Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)
    Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreview

    ab2910 staining Caveolin-1 - Caveolae Marker in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).

    Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.

    See Abreview

プロトコール

  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (122)

    ab2910 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2910 は 122 報の論文で使用されています。

    • Cai X  et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
    • Martin GG  et al. High Glucose and Liver Fatty Acid Binding Protein Gene Ablation Differentially Impact Whole Body and Liver Phenotype in High-Fat Pair-Fed Mice. Lipids N/A:N/A (2020). PubMed: 32314395
    • Nie Y  et al. The Na/K-ATPase a1 and c-Src form signaling complex under native condition: A crosslinking approach. Sci Rep 10:6006 (2020). PubMed: 32265464
    • Barruet E  et al. Functionally heterogeneous human satellite cells identified by single cell RNA sequencing. Elife 9:N/A (2020). PubMed: 32234209
    • Hasan SS  et al. Endothelial Notch signaling controls insulin transport in muscle. EMBO Mol Med 12:e09271 (2020). PubMed: 32187826
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 65 Abreviews or Q&A

    Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Dopaminergic Neurons)
    Gel Running Conditions
    Reduced Denaturing (NuPage 4-12% MES buffer)
    Loading amount
    30 µg
    Specification
    Dopaminergic Neurons
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 20 2020

    Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (kidney)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    30 µg
    Specification
    kidney
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 13 2020

    Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Fractions from mouse liver tissue to obtain lipid)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    Fractions from mouse liver tissue to obtain lipid
    Blocking step
    BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 18 2019

    Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (human Alveolar Epithelial cells (hAELVi))
    Gel Running Conditions
    Reduced Denaturing (4-12%)
    Loading amount
    1e+006 cells
    Specification
    human Alveolar Epithelial cells (hAELVi)
    Blocking step
    Western Breeze Blocking Solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 30 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (human Alveolar Epithelial cells (hAELVi))
    Permeabilization
    Yes - Saponin
    Specification
    human Alveolar Epithelial cells (hAELVi)
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 19 2018

    Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    50 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Juan Rodríguez Vita

    Verified customer

    投稿 Mar 27 2017

    Western blot abreview for Anti-Caveolin-1 antibody - Caveolae Marker

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3 fibroblasts)
    Gel Running Conditions
    Reduced Denaturing (15%)
    Loading amount
    10 µg
    Specification
    3T3 fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Mar 01 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Goat Tissue sections (Myocardium)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Specification
    Myocardium
    Blocking step
    (agent) for 10 minute(s) · Concentration: 2% · Temperature: 21°C
    Fixative
    Formaldehyde
    Read More

    Mr. Carl Hobbs

    Verified customer

    投稿 Jan 28 2016

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (neuron)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    neuron
    Blocking step
    Serum as blocking agent for 40 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 13 2015

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Caveolin-1 antibody - Caveolae Marker

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    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (COS-7)
    Permeabilization
    Yes - 0.2%Triton
    Specification
    COS-7
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
    Fixative
    Formaldehyde
    Read More

    Dr. Ya Zhou

    Verified customer

    投稿 Oct 06 2015

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