Anti-Caveolin-1 抗体 - Caveolae Marker (ab2910)
Key features and details
- Rabbit polyclonal to Caveolin-1 - Caveolae Marker
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Mouse, Rat, Hamster, Dog, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Caveolin-1 antibody - Caveolae Marker
Caveolin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caveolin-1 - Caveolae Marker -
由来種
Rabbit -
特異性
Detects caveolin-1. -
アプリケーション
適用あり: ICC, WBmore details -
種交差性
交差種: Mouse, Rat, Hamster, Dog, Human
交差が予測される動物種: Sheep, Rabbit, Horse, Cow, Cat, Pig, Chimpanzee, Gorilla, African green monkey, African bush elephant
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免疫原
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ポジティブ･コントロール
- WB: Human lung, heart and spleen tissue lysates; Rat heart protein extract. ICC: HeLa and NIH/3T3 cells; Rat astrocytes.
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特記事項
This antibody can be used as a marker for lipid raft fractions.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab2910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928). |
ターゲット情報
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機能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
組織特異性
Expressed in muscle and lung, less so in liver, brain and kidney. -
関連疾患
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
配列類似性
Belongs to the caveolin family. -
翻訳後修飾
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
細胞内局在
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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参照データベース
- Entrez Gene: 493668 Cat
- Entrez Gene: 463670 Chimpanzee
- Entrez Gene: 281040 Cow
- Entrez Gene: 403980 Dog
- Entrez Gene: 101125469 Gorilla
- Entrez Gene: 857 Human
- Entrez Gene: 12389 Mouse
- Entrez Gene: 404693 Pig
see all -
別名
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
画像
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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml
Lane 1 : Human lung
Lane 2 : Human heart
Lane 3 : Human spleen
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)ab2910 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2910 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 2 µg/ml + Rat heart protein extract
Predicted band size: 20 kDa -
Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)ab2910 at a 1/250 dilution staining NIH/3T3 (Mouse embryo fibroblast cell line) cells by Immunocytochemistry (panel B).
The antibody was incubated with the paraformaldehyde fixed cells for 12 hours. Bound antibody was detected using an Alexa Fluor ® 594 conjugated Goat anti-rabbit antibody (ab150080). Panel A shows staining with a mouse anti-Caveolin 1 antibody (clone 2297). Panel C shows the merged image.
This image is courtesy of an Abreview by William Ackerman.
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Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Rat astrocytes stained with fluorescently labeled Caveolin-1 antibody.
Primary antibody is ab2910 at a dilution of 1/500 and the secondary antibody is Texas red labeled anti-rabbit IgG at a dilution of 1/1000.
This image was kindly supplied as part of the review submitted by Donghui Zhu.
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Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreviewab2910 staining Caveolin-1 - Caveolae Marker in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.
プロトコール
参考文献 (122)
ab2910 は 122 報の論文で使用されています。
- Cai X et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
- Martin GG et al. High Glucose and Liver Fatty Acid Binding Protein Gene Ablation Differentially Impact Whole Body and Liver Phenotype in High-Fat Pair-Fed Mice. Lipids N/A:N/A (2020). PubMed: 32314395
- Nie Y et al. The Na/K-ATPase a1 and c-Src form signaling complex under native condition: A crosslinking approach. Sci Rep 10:6006 (2020). PubMed: 32265464
- Barruet E et al. Functionally heterogeneous human satellite cells identified by single cell RNA sequencing. Elife 9:N/A (2020). PubMed: 32234209
- Hasan SS et al. Endothelial Notch signaling controls insulin transport in muscle. EMBO Mol Med 12:e09271 (2020). PubMed: 32187826
