Anti-Caveolin-1 抗体 - Caveolae Marker (ab2910)
Key features and details
- Rabbit polyclonal to Caveolin-1 - Caveolae Marker
- Suitable for: ICC, WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Caveolin-1 antibody - Caveolae Marker
Caveolin-1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Caveolin-1 - Caveolae Marker -
由来種
Rabbit -
特異性
Detects caveolin-1. -
Tested Applications & Species
See all applications and species dataApplication Species ICC RatHumanWB RatHuman
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免疫原
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ポジティブ･コントロール
- WB: Human lung, heart and spleen tissue lysates; Rat heart protein extract. ICC: HeLa and NIH/3T3 cells; Rat astrocytes.
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特記事項
This antibody can be used as a marker for lipid raft fractions.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| アプリケーション | Species |
|---|---|
| ICC |
Rat
Human
|
| WB |
Rat
Human
|
| All applications |
Sheep
Rabbit
Horse
Cow
Cat
Pig
Chimpanzee
Gorilla
African green monkey
African bush elephant
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| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC |
Use at an assay dependent concentration.
|
|
| WB | (22) |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928).
|
| 特記事項 |
|---|
|
ICC
Use at an assay dependent concentration. |
|
WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 20 kDa).Can be blocked with Human Caveolin-1 peptide (ab4928). |
ターゲット情報
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機能
May act as a scaffolding protein within caveolar membranes. Interacts directly with G-protein alpha subunits and can functionally regulate their activity (By similarity). Involved in the costimulatory signal essential for T-cell receptor (TCR)-mediated T-cell activation. Its binding to DPP4 induces T-cell proliferation and NF-kappa-B activation in a T-cell receptor/CD3-dependent manner. Recruits CTNNB1 to caveolar membranes and may regulate CTNNB1-mediated signaling through the Wnt pathway. -
組織特異性
Expressed in muscle and lung, less so in liver, brain and kidney. -
関連疾患
Defects in CAV1 are the cause of congenital generalized lipodystrophy type 3 (CGL3) [MIM:612526]; also called Berardinelli-Seip congenital lipodystrophy type 3 (BSCL3). Congenital generalized lipodystrophies are autosomal recessive disorders characterized by a near absence of adipose tissue, extreme insulin resistance, hypertriglyceridemia, hepatic steatosis and early onset of diabetes. -
配列類似性
Belongs to the caveolin family. -
翻訳後修飾
The initiator methionine for isoform Beta is removed during or just after translation. The new N-terminal amino acid is then N-acetylated. -
細胞内局在
Golgi apparatus membrane. Cell membrane. Membrane > caveola. Membrane raft. Colocalized with DPP4 in membrane rafts. Potential hairpin-like structure in the membrane. Membrane protein of caveolae. - Information by UniProt
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参照データベース
- Entrez Gene: 493668 Cat
- Entrez Gene: 463670 Chimpanzee
- Entrez Gene: 281040 Cow
- Entrez Gene: 101125469 Gorilla
- Entrez Gene: 857 Human
- Entrez Gene: 404693 Pig
- Entrez Gene: 100008837 Rabbit
- Entrez Gene: 25404 Rat
see all -
別名
- BSCL3 antibody
- CAV antibody
- CAV1 antibody
see all
画像
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Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)All lanes : Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 1.5 µg/ml
Lane 1 : Human lung
Lane 2 : Human heart
Lane 3 : Human spleen
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Alexa Fluor anti-rabbit at 1/5000 dilution
Predicted band size: 20 kDa
Observed band size: 20 kDa -
Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)ab2910 staining Caveolin-1 in wild-type HeLa cells (top panel) and CAV1 knockout HeLa cells (ab255371) (bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab2910 at 1/500 dilution and ab7291 (Mouse monoclonal to alpha Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and a goat secondary antibody to mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8). -
Western blot - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Anti-Caveolin-1 antibody - Caveolae Marker (ab2910) at 2 µg/ml + Rat heart protein extract
Predicted band size: 20 kDa -
Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)Rat astrocytes stained with fluorescently labeled Caveolin-1 antibody.
Primary antibody is ab2910 at a dilution of 1/500 and the secondary antibody is Texas red labeled anti-rabbit IgG at a dilution of 1/1000.
This image was kindly supplied as part of the review submitted by Donghui Zhu.
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Immunocytochemistry - Anti-Caveolin-1 antibody - Caveolae Marker (ab2910)This image is courtesy of an anonymous Abreviewab2910 staining Caveolin-1 - Caveolae Marker in HeLa (Human epithelial cell line from cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence).
Cells were fixed with paraformaldehyde. Samples were incubated with primary antibody (1/200 in PBS + 0.05% Saponin) for 1 hour at 37°C. A Cy3®-conjugated Donkey anti-rabbit polyclonal (1/500) was used as the secondary antibody.
プロトコール
参考文献 (122)
ab2910 は 122 報の論文で使用されています。
- Cai X et al. miR-124a enhances therapeutic effects of bone marrow stromal cells transplant on diabetic nephropathy-related epithelial-to-mesenchymal transition and fibrosis. J Cell Biochem 121:299-312 (2020). PubMed: 31190436
- Martin GG et al. High Glucose and Liver Fatty Acid Binding Protein Gene Ablation Differentially Impact Whole Body and Liver Phenotype in High-Fat Pair-Fed Mice. Lipids N/A:N/A (2020). PubMed: 32314395
- Nie Y et al. The Na/K-ATPase a1 and c-Src form signaling complex under native condition: A crosslinking approach. Sci Rep 10:6006 (2020). PubMed: 32265464
- Barruet E et al. Functionally heterogeneous human satellite cells identified by single cell RNA sequencing. Elife 9:N/A (2020). PubMed: 32234209
- Hasan SS et al. Endothelial Notch signaling controls insulin transport in muscle. EMBO Mol Med 12:e09271 (2020). PubMed: 32187826
