Application
Western blot
Sample
Bos taurus Cell lysate - other (HEK293T cells and BoMAC (bovine macrophages) Lyse)
Gel Running Conditions
Reduced Denaturing
Loading amount
15 µg
Specification
HEK293T cells and BoMAC (bovine macrophages) Lyse
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/500
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: HRP-conjugated rabbit anti mouse (Dako)
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
20 minute(s) and 0 second(s)
Bands
Specific: none kDa Non-specific: 120 kDa (soluble), m kDa
Positive control
HEK293T lysates (human cells). bands of 120 + 35 kDa observed in soluble fraction. None of predicted 21 kDa.
BoMAC (bovine macrophages) - similar pattern as HEK293T cells.
Additional data
Abcam response
Thank you for participating in our Abtrial program and your honest feedback abut the use of ab17052 in bovine macrophages. Despite the high homology between the immunogen and bovine Calveolin, the antibody may not be suitable for this species.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 08 2016