Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse Calvarial)
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
25 µg
Treatment
Insulin as positive control
Specification
Mouse Calvarial
Blocking step
Odyssey Blocking Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Odyssey Blocking Buffer + 0.2% Tween
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: IRDye 800CW Goat (polyclonal) anti-Rabbit IgG (H
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Infrared
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 39 kDa Non-specific: 50, 76, 31 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 13 2015