Anti-Cathepsin D 抗体 [CTD-19] (ab6313)

Reviews (16)Q&A (11)References (46)

Key features and details

  • Mouse monoclonal [CTD-19] to Cathepsin D
  • Suitable for: WB
  • Reacts with: Mouse, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-Cathepsin D antibody [CTD-19]
    Cathepsin D 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [CTD-19] to Cathepsin D

  • 由来種

    Mouse

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Human

  • 免疫原

    Full length native protein (purified) (Human liver).

  • ポジティブ･コントロール

    • Human breast carcinoma tissue or cell line extract.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab6313 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Detects a band of approximately 52 kDa.

ターゲット情報

  • 機能

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

  • 組織特異性

    Expressed in the aorta extrcellular space (at protein level).

  • 関連疾患

    Ceroid lipofuscinosis, neuronal, 10

  • 配列類似性

    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.

  • 翻訳後修飾

    N- and O-glycosylated.

  • 細胞内局在

    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Information by UniProt

  • 参照データベース

    • 別名

      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    画像

    • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous Abreview
      Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg

      Secondary
      HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 28,48,50 kDa
      why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      Blocked with 5% milk for 1 hour at 22°C.

      Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

      This image was generated using the ascites version of the product.

      See Abreview

    • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells


      This image was generated using the ascites version of the product.

    参考文献 (46)

    ab6313 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6313 は 46 報の論文で使用されています。

    • Chrzanowski U  et al. Evaluation strategy to determine reliable demyelination in the cuprizone model. Metab Brain Dis 34:681-685 (2019). PubMed: 30607821
    • Koike S & Jahn R SNAREs define targeting specificity of trafficking vesicles by combinatorial interaction with tethering factors. Nat Commun 10:1608 (2019). PubMed: 30962439
    • Pauwels AM  et al. Spatiotemporal Changes of the Phagosomal Proteome in Dendritic Cells in Response to LPS Stimulation. Mol Cell Proteomics 18:909-922 (2019). PubMed: 30808727
    • Wang LL  et al. Downregulation of long noncoding RNA LINC01419 inhibits cell migration, invasion, and tumor growth and promotes autophagy via inactivation of the PI3K/Akt1/mTOR pathway in gastric cancer. Ther Adv Med Oncol 11:1758835919874651 (2019). PubMed: 31579114
    • Spengler D  et al. Novel therapeutic roles for surfactant-inositols and -phosphatidylglycerols in a neonatal piglet ARDS model: a translational study. Am J Physiol Lung Cell Mol Physiol 314:L32-L53 (2018). PubMed: 28860142
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 27 Abreviews or Q&A

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    25 µg
    Treatment
    5 µg/ml LPS, 24 h
    Specification
    cardiomyocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    投稿 Sep 16 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Treatment
    1 µg/ml LPS, 24 h
    Specification
    cardiomyocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Dr. Barbara Thaler

    Verified customer

    投稿 Sep 16 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin D antibody [CTD-19]

     Good
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (macrophages)
    Permeabilization
    Yes - 0.1% triton
    Specification
    macrophages
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Jul 18 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    投稿 Jul 09 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (CSF)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    CSF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 11 2015

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Good
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain tissue)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 11 2015

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Excellent
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Human Cell lysate - other (Glioblastoma cell lines)
    Specification
    Glioblastoma cell lines
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Mr. Achilleas Mitrakas

    Verified customer

    投稿 Apr 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin D antibody [CTD-19]

     Excellent
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (DU145 Human prostate)
    Specification
    DU145 Human prostate
    Permeabilization
    Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
    Fixative
    Paraformaldehyde
    Read More

    Dr. Dimitra Kalamida

    Verified customer

    投稿 Nov 21 2013

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

     Average
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (adipocyte tissue)
    Loading amount
    35 µg
    Specification
    adipocyte tissue
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Oct 18 2012

    Question

    Sehr geehrter Herr Hryca,
    vielen lieben Dank für das Angebot. Wir würden gerne die Maus-Antikörper
    bestellen und dabei ist uns aufgefallen, dass wir keine Möglichkeit einer
    Positivkontrolle haben. Können Sie uns da vielleicht weiterhelfen?
    Mit freundlichen Grüßen aus Düsseldorf

    Read More
    Answer

    Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

    Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von positive Kontrolle Sie benötigen? Ist es ein Lysat für Western blot oder ein Gewebeschnitt für Immunohistochemie? Haben Sie auch eine Präferenz für eine Spezies (Maus, human)?

    Hier mal eine Aufstellung von möglichen positiven Kontrollen. Ich freue mich wieder von Ihnen zu hören, um Ihnen genaure Vorschläge machen zu können.




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    1-10 of 27 Abreviews or Q&A

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