Anti-Cathepsin D 抗体 [CTD-19] (ab6313)
![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.co.jp/ps/products/6/ab6313/Images/ab6313-275029-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg "Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)")
Key features and details
- Mouse monoclonal [CTD-19] to Cathepsin D
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2a
製品の概要
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製品名
Anti-Cathepsin D antibody [CTD-19]
Cathepsin D 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CTD-19] to Cathepsin D -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Full length native protein (purified) corresponding to Human Cathepsin D.
Database link: P07339 -
ポジティブ･コントロール
- Human breast carcinoma tissue or cell line extract.
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特記事項
This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.097% Sodium azide -
Concentration information loading... -
精製度
Proprietary Purification -
特記事項（精製）
Purified from TCS -
ポリ/モノ
モノクローナル -
クローン名
CTD-19 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab6313の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (12) |
Use at an assay dependent concentration. Detects a band of approximately 52 kDa.
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| 特記事項 |
|---|
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WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa. |
ターゲット情報
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機能
Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. -
組織特異性
Expressed in the aorta extrcellular space (at protein level). -
関連疾患
Ceroid lipofuscinosis, neuronal, 10 -
配列類似性
Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻訳後修飾
N- and O-glycosylated. -
細胞内局在
Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380). - Information by UniProt
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参照データベース
- Entrez Gene: 1509 Human
- Omim: 116840 Human
- SwissProt: P07339 Human
- Unigene: 654447 Human
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別名
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
画像
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Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous AbreviewAnti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg
Secondary
HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?
Exposure time: 2 minutesBlocked with 5% milk for 1 hour at 22°C.
Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.
This image was generated using the ascites version of the product.
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![Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)](https://www.abcam.co.jp/ps/products/6/ab6313/Images/ab6313-12229-anti-cathepsin-d-antibody-ctd-19-western-blot.jpg)
Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cellsThis image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (57)
ab6313 は 57 報の論文で使用されています。
- Castroflorio E et al. The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. Cell Mol Life Sci 78:3503-3524 (2021). PubMed: 33340069
- Kim S et al. Endosomal mTORC2 Is Required for Phosphoinositide-Dependent AKT Activation in Platelet-Derived Growth Factor-Stimulated Glioma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 34065746
- Kim SY et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
- Feng Y et al. Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis 11:513 (2020). PubMed: 32641688
- Ghosh S et al. ß-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway. Cell 183:1520-1535.e14 (2020). PubMed: 33157038