アブカムでは最適な動作のために Google Chrome など最新ブラウザでの閲覧を推奨します。

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
お問い合わせは電話 +81-(0)3-6231-0940 または メールでどうぞ

  • アカウント
  • ログアウト
ログイン または 新規登録

Welcome

ログイン または

すでにアカウントをお持ちですか?

新規登録
カート
クイック・オーダー
Abcam homepage

  • 研究用製品
    製品タイプ別
    一次抗体
    二次抗体
    イムノアッセイキット・試薬
    細胞・組織イメージング
    細胞・バイオケミカルアッセイ
    タンパク質・ペプチド
    製品タイプ別
    プロテオミクス
    アゴニスト・アクチベータ-・アンタゴニスト・インヒビター
    細胞株・ライゼート
    miRNA マルチプレックス・アッセイキット
    マルチプレックス・アッセイ
    研究分野別
    癌
    循環器系
    細胞生物学
    エピジェネティクス
    メタボリズム
    発生生物学
    研究分野別
    免疫学
    微生物学
    脳神経科学
    シグナル伝達
    ステムセル
  • カスタム製品 & パートナーシップ
    カスタム製品 & パートナーシップ

    診断や治療プログラムを加速するための、カスタム製品および商業化に向けたパートナーシップ

    カスタム製品

    私たちとパートナーになる

  • サポート
    サポートハブ

    研究のためのサポートとアドバイス

    サポートハブを表示

    プロトコール

    実験のステップごとの詳細

    プロトコールを表示

  • イベント
    • イベント・カレンダー
    • 癌
    • 循環器
    • エピジェネティクス&核内情報
    • 免疫学
    • ニューロサイエンス
    • ステムセル
    • 展示会
    • ウェビナー
    最新のイベント情報

    世界中でアブカムが主催する研究会やセミナーの日程、内容、演者など

    イベント・カレンダー

  • パスウェイ
    細胞シグナル伝達パスウェイ

    すべてのパスウェイを見る

    インタラクティブ・パスウェイを見る

  1. Link

    cathepsin-d-antibody-ctd-19-ab6313.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Neuroscience Cell Adhesion Proteins Membrane Proteins
Share by email

Anti-Cathepsin D 抗体 [CTD-19] (ab6313)

  • Datasheet
  • SDS
Reviews (16)Q&A (11)References (57)

Product price, shipping and contact information

Currently unavailable

申し訳ございませんが、現在表示できません。

こちらにお問い合わせいただくか、またはしばらく経ってから改めてお試しください

 

Loading size & price…

 

出荷および受注について

Shipping info

Promotion Information

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
  • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)

Key features and details

  • Mouse monoclonal [CTD-19] to Cathepsin D
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG2a

こちらの製品もご検討ください

一次抗体
Product image
Anti-CaSR antibody (ab137408)
タンパク質
Recombinant Mouse Cathepsin D protein (ab191943)
一次抗体
Product image
Alexa Fluor® 647 Anti-Ubiquitin (linkage-specific K63) antibody [EPR8590-448] (ab192489)

関連製品

製品の概要

  • 製品名

    Anti-Cathepsin D antibody [CTD-19]
    Cathepsin D 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [CTD-19] to Cathepsin D
  • 由来種

    Mouse
  • アプリケーション

    適用あり: WBmore details
  • 種交差性

    交差種: Human
  • 免疫原

    Full length native protein (purified) corresponding to Human Cathepsin D.
    Database link: P07339

  • ポジティブ・コントロール

    • Human breast carcinoma tissue or cell line extract.
  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 21 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.097% Sodium azide
  • Concentration information loading...
  • 精製度

    Proprietary Purification
  • 特記事項(精製)

    Purified from TCS
  • ポリ/モノ

    モノクローナル
  • クローン名

    CTD-19
  • アイソタイプ

    IgG2a
  • 研究分野

    • Neuroscience
    • Cell Adhesion Proteins
    • Membrane Proteins
    • Signal Transduction
    • Cytoskeleton / ECM
    • Extracellular Matrix
    • ECM Enzymes
    • Other Enzymes
    • Cancer
    • Invasion/microenvironment
    • ECM
    • Extracellular matrix
    • Other
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteolytic enzymes
    • Cysteine protease
    • Cathepsins
    • Neuroscience
    • Diseases
    • Neuroscience
    • Processes

関連製品

  • Assay kits

    • Cathepsin D Inhibitor Assay Kit (ab126779)
    • Cathepsin D Activity Assay Kit (Fluorometric) (ab65302)
  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
  • Recombinant Protein

    • Recombinant Mouse Cathepsin D protein (ab191943)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab6313の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB (12)
Use at an assay dependent concentration. Detects a band of approximately 52 kDa.
特記事項
WB
Use at an assay dependent concentration. Detects a band of approximately 52 kDa.

ターゲット情報

  • 機能

    Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
  • 組織特異性

    Expressed in the aorta extrcellular space (at protein level).
  • 関連疾患

    Ceroid lipofuscinosis, neuronal, 10
  • 配列類似性

    Belongs to the peptidase A1 family.
    Contains 1 peptidase A1 domain.
  • 翻訳後修飾

    N- and O-glycosylated.
  • 細胞内局在

    Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
  • Target information above from: UniProt accession P07339 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 1509 Human
    • Omim: 116840 Human
    • SwissProt: P07339 Human
    • Unigene: 654447 Human
    • 別名

      • CatD antibody
      • CATD_HUMAN antibody
      • Cathepsin D antibody
      • Cathepsin D heavy chain antibody
      • CD antibody
      • Ceroid lipofuscinosis neuronal 10 antibody
      • CLN10 antibody
      • CPSD antibody
      • ctsd antibody
      • Epididymis secretory sperm binding protein Li 130P antibody
      • HEL S 130P antibody
      • Lysosomal aspartyl peptidase antibody
      • Lysosomal aspartyl protease antibody
      • MGC2311 antibody
      see all

    画像

    • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)This image is courtesy of an anonymous Abreview
      Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/1000 dilution + MDA MB 231 whole cell lysate at 20 µg

      Secondary
      HRP-conjugated monoclonal sheep anti-mouse IgG at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Observed band size: 28,48,50 kDa why is the actual band size different from the predicted?


      Exposure time: 2 minutes


      Blocked with 5% milk for 1 hour at 22°C.

      Incubated with the primary antibody for 1 hour at 22°C in 5% TBS-T.

      This image was generated using the ascites version of the product.

      See Abreview

    • Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Western blot - Anti-Cathepsin D antibody [CTD-19] (ab6313)
      Anti-Cathepsin D antibody [CTD-19] (ab6313) at 1/4000 dilution + MCF7 Human breast carcinoma cells


      This image was generated using the ascites version of the product.

    プロトコール

    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • SDS download

    • Datasheet download

      Download

    参考文献 (57)

    ab6313 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab6313 は 57 報の論文で使用されています。

    • Castroflorio E  et al. The Ncoa7 locus regulates V-ATPase formation and function, neurodevelopment and behaviour. Cell Mol Life Sci 78:3503-3524 (2021). PubMed: 33340069
    • Kim S  et al. Endosomal mTORC2 Is Required for Phosphoinositide-Dependent AKT Activation in Platelet-Derived Growth Factor-Stimulated Glioma Cells. Cancers (Basel) 13:N/A (2021). PubMed: 34065746
    • Kim SY  et al. Non-Thermal Plasma Induces Antileukemic Effect Through mTOR Ubiquitination. Cells 9:N/A (2020). PubMed: 32131492
    • Feng Y  et al. Rab27a dependent exosome releasing participated in albumin handling as a coordinated approach to lysosome in kidney disease. Cell Death Dis 11:513 (2020). PubMed: 32641688
    • Ghosh S  et al. ß-Coronaviruses Use Lysosomes for Egress Instead of the Biosynthetic Secretory Pathway. Cell 183:1520-1535.e14 (2020). PubMed: 33157038
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 27 Abreviews or Q&A

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    25 µg
    Treatment
    5 µg/ml LPS, 24 h
    Specification
    cardiomyocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Barbara Thaler

    Verified customer

    投稿 Sep 16 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (cardiomyocytes)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Loading amount
    15 µg
    Treatment
    1 µg/ml LPS, 24 h
    Specification
    cardiomyocytes
    Blocking step
    Milk as blocking agent for 4 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Dr. Barbara Thaler

    Verified customer

    投稿 Sep 16 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (macrophages)
    Permeabilization
    Yes - 0.1% triton
    Specification
    macrophages
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Jul 18 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (C42B Prostate cancer cell line)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    C42B Prostate cancer cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    投稿 Jul 09 2019

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Tissue lysate - whole (CSF)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    CSF
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 11 2015

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (brain tissue)
    Gel Running Conditions
    Reduced Denaturing (12)
    Loading amount
    50 µg
    Specification
    brain tissue
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Aug 11 2015

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Non-reduced Denaturing (10)
    Sample
    Human Cell lysate - other (Glioblastoma cell lines)
    Specification
    Glioblastoma cell lines
    Blocking step
    Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    MR. Achilleas Mitrakas

    Verified customer

    投稿 Apr 25 2014

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Cathepsin D antibody [CTD-19]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Blocking step
    BSA as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: RT°C
    Sample
    Human Cell (DU145 Human prostate)
    Specification
    DU145 Human prostate
    Permeabilization
    Yes - 0.1% v/v Triton X-100 pH 7.4 for 5 min at RT
    Fixative
    Paraformaldehyde
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    DR. Dimitra Kalamida

    Verified customer

    投稿 Nov 21 2013

    Western blot abreview for Anti-Cathepsin D antibody [CTD-19]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (adipocyte tissue)
    Loading amount
    35 µg
    Specification
    adipocyte tissue
    Gel Running Conditions
    Reduced Denaturing (10% gel)
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More
    The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

    Abcam user community

    Verified customer

    投稿 Oct 18 2012

    Question

    Sehr geehrter Herr Hryca,
    vielen lieben Dank für das Angebot. Wir würden gerne die Maus-Antikörper
    bestellen und dabei ist uns aufgefallen, dass wir keine Möglichkeit einer
    Positivkontrolle haben. Können Sie uns da vielleicht weiterhelfen?
    Mit freundlichen Grüßen aus Düsseldorf

    Read More

    Abcam community

    Verified customer

    Asked on Aug 14 2012

    Answer

    Vielen Dank für Ihre Anfrage die Herr Hryca uns in den technischen Dienst weitergeleitet hat.

    Ich habe geschaut, und wir haben viele möglichen positiven Kontrollen für Sie. Können Sie mir bitte noch sagen, welche Art von positive Kontrolle Sie benötigen? Ist es ein Lysat für Western blot oder ein Gewebeschnitt für Immunohistochemie? Haben Sie auch eine Präferenz für eine Spezies (Maus, human)?

    Hier mal eine Aufstellung von möglichen positiven Kontrollen. Ich freue mich wieder von Ihnen zu hören, um Ihnen genaure Vorschläge machen zu können.




    cell lines Slide
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=2105 Rabbit polyclonal to IL1 beta LPS treated RAW 264.7 cells, Alveolar macrophages (MHS cell line) liver, diseased, inflammed tissue, monocytes, macrophages
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=6313 Mouse monoclonal [CTD-19] to Cathepsin D SKBR3, MCF7 Human liver tissue.
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=6535 Mouse monoclonal [BRM-22] to Hsp70 Hela, 293T,MCF7, Jurkat, HepG2, A431, HCT116, PC3, ... liver, brain, testes, heart, skeletal muscle
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=8219 Mouse monoclonal [MEM-259] to CD63 HT1080, HepG2, OVCAR-3, HL60 and A375 hemapoietic ells, tissue macrophages, platelet, human melanoma tissue, monocytes, macrophages, endothelial cells, SM cells, brain white matter, peripheral nerves
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=25631 Mouse monoclonal [H4B4] to LAMP2 - Lysosome Marker HepG2, 3T3, HeLa, RAW264.7, ... placenta, lung, liver
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=50533 Mouse monoclonal [Rab7-117] to RAB7 HepG2, B16, SK-MEL-28, 3T3, Human brain whole tissue, mouse neuroblastoma
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=68340 Mouse monoclonal to Rab9 Hela, A172, 3T3, 293, PC-12. COS-7, THP-1 tonsil, spleen, prostate cancer, stomach tissu
    http://ops.adminsite.com/admin/data/fs_ab.cfm?intAbID=72907 Rabbit polyclonal to Rab5b Hela, Jurkat, HEK293, HepG2 Human Placenta Tissue, Human Brain Tissue.

    Read More

    Abcam Scientific Support

    Answered on Aug 14 2012

    1-10 of 27 Abreviews or Q&A

    •  戻る
    • 1
    • 2
    • 3
    • 次へ 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    技術情報や製品情報をメールで受け取る 登録
    製品・研究分野別
    • 癌
    • 循環器
    • 細胞生物学
    • 発生
    • エピジェネティクス&核内情報
    • 免疫学
    • メタボリズム
    • 感染症
    • ニューロサイエンス
    • シグナル伝達
    • ステムセル
    製品・タイプ別
    • 一次抗体
    • 二次抗体
    • バイオケミカルズ
    • アイソタイプ・コントロール
    • マルチカラー・セレクター
    • キット
    • ローディング・コントロール
    • ライゼート
    • ペプチド
    • タンパク質
    • スライド
    • タグ抗体・細胞マーカー
    • その他試薬
    サービス&サポート
    • サポート
    • よくあるお問い合わせ
    • プロトコール&トラブルシュート
    • ご購入に関して
    • お問い合わせフォーム
    • トレーニング
    • ターゲット別に見る
    アブカムについて
    • 企業ウェブサイト
    • 投資家の皆様へ
    • 企業情報
    • 採用情報
    • アブカムについて
    イベント
    • 展示会
    • カンファレンス
    International websites
    • abcam.com
    • abcam.cn

    Join with us

    • LinkedIn
    • facebook
    • Twitter
    • YouTube
    • Terms of sale
    • Website terms of use
    • Cookie policy
    • Privacy policy
    • Legal
    © 1998-2022 Abcam plc. All rights reserved.