製品の概要
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製品名Anti-Cathepsin D antibody
Cathepsin D 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to Cathepsin D
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由来種Rabbit
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特異性This antibody reacts with the zymogen as well as the activated form of cathepsin D. It stains fibroblasts and macrophages strongly but stains weakly with the myoepithelial cells of breast. It also stains sweat ducts and glands in the skin, islets cells and granules of pancreatic acini.
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アプリケーション適用あり: IHC-P, IHC-Fr, IHC-FoFr, ICC/IFmore details
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種交差性交差種: Rat, Human
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免疫原
Cathepsin D isolated from human liver
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ポジティブ･コントロール
- ICC/IF: A431 cells.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.05% Sodium Azide
Constituents: 1% BSA -
Concentration information loading... -
精製度Protein A purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Assay kits
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab826 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IHC-P | 1/75 - 1/100. ABC method. | |
| IHC-Fr | 1/75 - 1/100. ABC method. | |
| IHC-FoFr | Use at an assay dependent concentration. PubMed: 19505575 | |
| ICC/IF | Use a concentration of 10 µg/ml. |
ターゲット情報
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機能Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
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組織特異性Expressed in the aorta extrcellular space (at protein level).
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関連疾患Ceroid lipofuscinosis, neuronal, 10
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配列類似性Belongs to the peptidase A1 family.
Contains 1 peptidase A1 domain. -
翻訳後修飾N- and O-glycosylated.
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細胞内局在Lysosome. Melanosome. Secreted, extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix (PubMed:20551380).
- Information by UniProt
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参照データベース
- Entrez Gene: 1509 Human
- Entrez Gene: 171293 Rat
- Omim: 116840 Human
- SwissProt: P07339 Human
- SwissProt: P24268 Rat
- Unigene: 654447 Human
- Unigene: 11085 Rat
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別名
- CatD antibody
- CATD_HUMAN antibody
- Cathepsin D antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-Cathepsin D antibody (ab826)ICC/IF image of ab826 stained A431 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab826, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cathepsin D antibody (ab826)ab826 staining Cathepsin D in formalin-fixed, paraffin-embedded Human prostate tissue by Immunohistochemistry. -
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Cathepsin D antibody (ab826)Image from Chu Y et al., Neurobiol Dis. 2009 Sep;35(3):385-98. doi: 10.1016/j.nbd.2009.05.023. Epub 2009 Jun 6.Fig 8.; Neurobiology of Disease 35 (2009) 385–398 with permission from Elsevier.Immunohistochemical analysis of PFA-perfusion fixed, frozen rat brain tissue sections (substantia nigra), staining Cathepsin D with ab826. Samples were incubated with primary antibody overnight at room temperature.
参考文献
This product has been referenced in:
- Minchew CL & Didenko VV Dual Detection of Nucleolytic and Proteolytic Markers of Lysosomal Cell Death: DNase II-Type Breaks and Cathepsin D. Methods Mol Biol 1554:229-236 (2017). Read more (PubMed: 28185196) »
- Takakura K et al. Cordyceps militaris improves the survival of Dahl salt-sensitive hypertensive rats possibly via influences of mitochondria and autophagy functions. Heliyon 3:e00462 (2017). WB ; Rat . Read more (PubMed: 29264419) »
レビューと Q&A
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