製品の概要

  • 製品名
    Anti-Cathepsin B antibody [CA10]
    Cathepsin B 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [CA10] to Cathepsin B
  • 由来種
    Mouse
  • 特異性
    This antibody recognizes an epitope on the heavy chain of mature Cathepsin B and therefore detects both mature (~31 kDa) and pro (~43 kDa)Cathepsin B.
  • アプリケーション
    適用あり: IHC-P, Flow Cyt, WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Cow, Human, Pig
  • 免疫原

    Cathepsin B purified from human liver

  • エピトープ
    Within the heavy chain of mature cathepsin B
  • ポジティブ・コントロール
    • HepG2 cells
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. We would recommend antibodies ab214428 or ab125067 as replacements.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/200.
Flow Cyt Use 0.5µg for 106 cells.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 0.25 µg/ml. Predicted molecular weight: 38 kDa.
IP Use a concentration of 1 µg/ml.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
  • 配列類似性
    Belongs to the peptidase C1 family.
  • 細胞内局在
    Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Amyloid precursor protein secretase antibody
    • APP secretase antibody
    • APPS antibody
    • CATB_HUMAN antibody
    • Cathepsin B heavy chain antibody
    • Cathepsin B1 antibody
    • CathepsinB antibody
    • CPSB antibody
    • CTSB antibody
    • cysteine protease antibody
    • OTTHUMP00000116009 antibody
    • OTTHUMP00000229510 antibody
    • OTTHUMP00000229511 antibody
    • OTTHUMP00000229512 antibody
    • OTTHUMP00000229514 antibody
    • OTTHUMP00000229515 antibody
    • OTTHUMP00000229516 antibody
    • Preprocathepsin B antibody
    see all

画像

  • Immunofluorescence analysis of U87MG cells incubated with netrin-1, staining Cathepsin B with ab58802.
  • Overlay histogram showing HepG2 cells stained with ab58802 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58802, 0.5µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • ab58802 staining Cathepsin B in murine mammary gland tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Samples were then blocked and then incubated with ab58802 at a 1/200 dilution for 30 minutes at 22°C. The secondary used was a biotin conjugated rabbit anti-mouse monoclonal at a 1/250 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Hsu SPC  et al. Temozolomide, sirolimus and chloroquine is a new therapeutic combination that synergizes to disrupt lysosomal function and cholesterol homeostasis in GBM cells. Oncotarget 9:6883-6896 (2018). Read more (PubMed: 29467937) »
  • Komura T  et al. Clinical features of cystatin A expression in patients with pancreatic ductal adenocarcinoma. Cancer Sci 108:2122-2129 (2017). IHC ; Human . Read more (PubMed: 28898495) »

See all 30 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (gall bladder cancer cell line OCUG-1)
Gel Running Conditions
Reduced Denaturing (12% SDS PAGE)
Loading amount
50 µg
Specification
gall bladder cancer cell line OCUG-1
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Mr. Siddharth Mehra

Verified customer

投稿 Jun 01 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (Mammary gland)
Specification
Mammary gland
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Permeabilization
No
Blocking step
Vector M.O.M. Blocking Reagent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3.2% · Temperature: 22°C
Username

Dr. Irina Teplova

Verified customer

投稿 Jul 18 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (SHSY5Y cell)
Loading amount
50 µg
Specification
SHSY5Y cell
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (A375 cells)
Loading amount
50 µg
Specification
A375 cells
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 31 2009

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH 3T3 cells)
Loading amount
10 µg
Specification
NIH 3T3 cells
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Mar 27 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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