Anti-Cathepsin B 抗体 (ab190077)
Key features and details
- Rabbit polyclonal to Cathepsin B
- Suitable for: IHC-P, WB, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-Cathepsin B antibody
Cathepsin B 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Cathepsin B -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Cathepsin B aa 198-227 (internal sequence) conjugated to keyhole limpet haemocyanin.
Database link: P07858 -
ポジティブ・コントロール
- WB: Y79 cell lysate. Flow Cyt (Intra): HepG2 cells. IHC-P: Human liver and hepatocarcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab190077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 10 µg/ml.
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WB |
1/1000. Predicted molecular weight: 38 kDa.
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Flow Cyt (Intra) |
1/10 - 1/50.
ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
特記事項 |
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IHC-P
Use a concentration of 10 µg/ml. |
WB
1/1000. Predicted molecular weight: 38 kDa. |
Flow Cyt (Intra)
1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. -
配列類似性
Belongs to the peptidase C1 family. -
細胞内局在
Lysosome. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 1508 Human
- Omim: 116810 Human
- SwissProt: P07858 Human
- Unigene: 520898 Human
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別名
- Amyloid precursor protein secretase antibody
- APP secretase antibody
- APPS antibody
see all
画像
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Anti-Cathepsin B antibody (ab190077) at 1/1000 dilution + Y79 cell lysate at 35 µg
Predicted band size: 38 kDa -
Intracellular flow cytometric analysis of HepG2cells labeling Cathepsin B with ab190077 at 1/10 dilution (lowerhistogram) compared to a negative control (upperhistogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human hepatocarcinoma tissue labeling Cathepsin B with ab190077 at 10 µg/ml followed by peroxidase-conjugated secondary antibody and DAB staining.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling Cathepsin B with ab190077 at 10 µg/ml.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (3)
ab190077 は 3 報の論文で使用されています。
- Rudzinska-Radecka M et al. In Silico, In Vitro, and Clinical Investigations of Cathepsin B and Stefin A mRNA Expression and a Correlation Analysis in Kidney Cancer. Cells 11:N/A (2022). PubMed: 35563761
- Rudzinska M et al. Cysteine Cathepsins Inhibition Affects Their Expression and Human Renal Cancer Cell Phenotype. Cancers (Basel) 12:N/A (2020). PubMed: 32455715
- Li W et al. Clomiphene citrate induces nuclear translocation of the TFEB transcription factor and triggers apoptosis by enhancing lysosomal membrane permeabilization. Biochem Pharmacol N/A:N/A (2018). PubMed: 30471247