Catalase beads (ab218718)
Key features and details
- Sample type: Cell Lysate, Plasma, Serum, Urine
製品の概要
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製品名
Catalase beads -
サンプルの種類
Urine, Serum, Plasma, Cell Lysate -
製品の概要
Catalase beads (ab218718) are used to remove hydrogen peroxide from biological samples prior to downstream analyses where the presence of hydrogen peroxide causes interference. They can be used on urine, serum, plasma or cellular extracts. ab218718 is well-suited for high throughput preparation protocols.
ab218718 is manufactured using a proprietary method to covalently attach catalase to 6% cross-linked agarose beads. The coupling technique is optimized to give a high yield and recovery of catalase activity, and to ensure long term stability of the immobilized enzyme.
ab218718 is available in 1 or 10 mL sizes of a 50% slurry of catalase agarose beads in PBS, with a capacity of 5 or 50 mL of sample, respectively.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 7931 Immobilized Catalase Beads. 7931-1 is the same size as the 1 ml size of ab218718.
Protocol
You will also require 96-well polypropylene plates and a centrifuge for 96-well plates.
- Mix catalase beads gently to generate a homogeneous suspension (do not invert the tube).
- Add 10 μL of the catalase bead suspension to 50 μL of your sample.
- Incubate the sample at room temperature for fifteen minutes.
- Spin down the sample at 1000 x g for a minute at room temperature to pellet the beads at the bottom of the tube.
- Carefully collect the supernatant, taking care not to disturb the bead pellet. The sample is now ready for analysis.
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アプリケーション
適用あり: Purificationmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 1 ml 10 ml Catalase Agarose Beads 1 x 1ml 1 x 10ml
関連製品
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab218718の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Purification |
Use at an assay dependent concentration.
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特記事項 |
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Purification
Use at an assay dependent concentration. |
画像
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Urine adenosine was measured using Abcam’s Adenosine Fluorometric Assay Kit (ab211094) in 2x diluted -untreated and -treated (immobilized catalase beads ab218718 + enzyme mix for ab211094) urine sample. There is a considerable decrease in the sample background in the treated urine as compared to the untreated urine. After background subtraction, linearity across sample volume can be observed in the treated urine sample, but not in the untreated urine sample.
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Urine adenosine was measured using Abcam’s Adenosine Fluorometric Assay Kit (ab211094) in 2x diluted -untreated and -treated (immobilized catalase beads ab218718 + enzyme mix for ab211094) urine sample. There is a considerable decrease in the sample background in the treated urine as compared to the untreated urine. In the ab211094 assay, intrinsic hydrogen peroxide and other substrates in the urine sample that also generate hydrogen peroxide are contributing to the background and the immobilized Catalase beads is minimizing such background.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab218718 は論文での使用が確認できていません。