Application
Western blot
Sample
Bat Cell lysate - whole cell (Huh7 (control), P. alecto brain, fetus, kidney and)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
50 µg
Specification
Huh7 (control), P. alecto brain, fetus, kidney and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk/TBST
Dilution
1/1000
Secondary antibody
Dilution
1/4000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit IgG (H+L)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa Non-specific: 70, 50, 40, 38 kDa
Positive control
Only saw predicted band size with the Huh7 cells, and only saw non-specific bands in the P. alecto cells (but not the Huh7 cells).
Additional data
Additional Notes
PVDF membrane
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 17 2016