Application
Western blot
Sample
Mouse Tissue lysate - whole (supernatant of liver homogenate)
Loading amount
5 µg
Specification
supernatant of liver homogenate
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Incubation time
3 hour(s) and 0 minute(s)
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit secondary antibody (NA 934)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
chemiluminescence
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
PVDF membrane was used; sample was not treated with any of the protease inhibitors; unspecific bands were observed only in overloaded samples; we used 5 micrograms of protein as a reference amount in our experiments
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Sandra Sobocanec
Verified customer
投稿 Mar 16 2006