Application
Western blot
Sample
Human Cell lysate - whole cell (Human cancer cell lines)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
Human cancer cell lines
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 21°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA, PBS-Tween 0.1%
Concentration
1 µg/ml
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Swine anti Rabbit HRP
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Pig
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
Supersignal west dura
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 60 kDa
Additional data
Additional Notes
Order: (1) 293T, (2) HeLa, (3) U251, (4) Jurkat, (5) U2OS
Detects a species of the predicted mobility in a range of human cancer lines.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Feb 21 2012