Anti-Caspase-9 抗体 [EPR18868] (ab184786)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR18868] to Caspase-9
- Suitable for: IP, WB
- Reacts with: Mouse, Rat
Related conjugates and formulations
製品の概要
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製品名
Anti-Caspase-9 antibody [EPR18868]
Caspase-9 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR18868] to Caspase-9 -
由来種
Rabbit -
アプリケーション
適用あり: IP, WBmore details -
種交差性
交差種: Mouse, Rat -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: C6 and NIH/3T3 whole cell lysates; Mouse brain, heart and spleen lysates; Rat brain and heart lysates. IP: NIH/3T3 and C6 treated with 1µM staurosporine for 4h whole cell lysates.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR18868 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Assay kits
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab184786の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
1/30.
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WB | (1) |
1/1000. Detects a band of approximately 50, 39, 37 kDa (predicted molecular weight: 50, 39, 37 kDa).
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特記事項 |
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IP
1/30. |
WB
1/1000. Detects a band of approximately 50, 39, 37 kDa (predicted molecular weight: 50, 39, 37 kDa). |
ターゲット情報
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機能
Involved in the activation cascade of caspases responsible for apoptosis execution. Binding of caspase-9 to Apaf-1 leads to activation of the protease which then cleaves and activates caspase-3. Proteolytically cleaves poly(ADP-ribose) polymerase (PARP).
Isoform 2 lacks activity is an dominant-negative inhibitor of caspase-9. -
組織特異性
Ubiquitous, with highest expression in the heart, moderate expression in liver, skeletal muscle, and pancreas. Low levels in all other tissues. Within the heart, specifically expressed in myocytes. -
配列類似性
Belongs to the peptidase C14A family.
Contains 1 CARD domain. -
発生段階
Expressed at low levels in fetal heart, at moderate levels in neonate heart, and at high levels in adult heart. -
翻訳後修飾
Cleavages at Asp-315 by granzyme B and at Asp-330 by caspase-3 generate the two active subunits. Caspase-8 and -10 can also be involved in these processing events. - Information by UniProt
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参照データベース
- Entrez Gene: 12371 Mouse
- Entrez Gene: 58918 Rat
- SwissProt: Q8C3Q9 Mouse
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別名
- APAF-3 antibody
- APAF3 antibody
- Apoptosis related cysteine peptidase antibody
see all
画像
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All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Untreated C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 0.5 hour
Lane 3 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour
Lane 4 : C6 whole cell lysate treated with 1 µm staurosporine for 4 hours
Lane 5 : C6 whole cell lysate treated with 50 µm Z-VAD-FMK for 1 hour, then treated with 1 µM staurosporine for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 10 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The p39 and p37 subunits were inhibited when the caspase activity was blocked by the caspase inhibitor, Z-VAD-FMK.
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All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Untreated NIH/3T3 (Mouse embyro fibroblast cell line) whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate treated with 1 µM staurosporine for 4 hours
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 15 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-Caspase-9 antibody [EPR18868] (ab184786) at 1/1000 dilution
Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse spleen lysate
Lane 4 : Rat brain lysate
Lane 5 : Rat heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 50, 39, 37 kDa
Observed band size: 37,39,50 kDa why is the actual band size different from the predicted?
Exposure time: 20 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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Caspase 9 was immunoprecipitated from 1mg of NIH/3T3 whole cell lysate (Mouse embyro fibroblast cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h,10ug (Input).
Lane 2: ab184786 IP in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184786 in NIH/3T3 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could not be observed even with 3 minutes exposure time.
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Caspase 9 was immunoprecipitated from 1mg of C6 whole cell lysate (Rat glial tumor cells) treated with 1μM staurosporine for 4h with ab184786 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab184786 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: C6 whole cell lysate treated with 1μM staurosporine for 4h, 10ug (Input).
Lane 2: ab184786 IP in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab184786 in C6 whole cell lysate treated with 1μM staurosporine for 4h.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 seconds.The product has better affinity to procaspase-9. The cleaved form p37 could be observed with longer exposure time.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (34)
ab184786 は 34 報の論文で使用されています。
- Azimi M et al. The Effect of Thymus vulgaris on Hepatic Enzymes Activity and Apoptosis-Related Gene Expression in Streptozotocin-Induced Diabetic Rats. Evid Based Complement Alternat Med 2022:2948966 (2022). PubMed: 35368767
- Fan K et al. Drp1-Mediated Mitochondrial Metabolic Dysfunction Inhibits the Tumor Growth of Pituitary Adenomas. Oxid Med Cell Longev 2022:5652586 (2022). PubMed: 35368865
- Li Y et al. Evodiamine suppresses the progression of non-small cell lung carcinoma via endoplasmic reticulum stress-mediated apoptosis pathway in vivo and in vitro. Int J Immunopathol Pharmacol 36:3946320221086079 (2022). PubMed: 35388733
- Li X et al. Ischemia-induced cleavage of OPA1 at S1 site aggravates mitochondrial fragmentation and reperfusion injury in neurons. Cell Death Dis 13:321 (2022). PubMed: 35395832
- Liu C & Zou Q miR-21 Regulates Immune Balance Mediated by Th17/Treg in Peripheral Blood of Septic Rats during the Early Phase through Apoptosis Pathway. Biochem Res Int 2022:9948229 (2022). PubMed: 35528843