Application
Western blot
Sample
Human Cell lysate - whole cell (U87 cells)
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (4-20%)
Loading amount
13 µg
Treatment
bevasizumab 0, 1 and 2mg/ml for 24hrs, 48hrs
Specification
U87 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
13 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 46 kDa
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投稿 May 11 2020