Anti-Caspase-8 p10 抗体 [C502S] (ab2553)
Key features and details
- Mouse monoclonal [C502S] to Caspase-8 p10
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-Caspase-8 p10 antibody [C502S] -
製品の詳細
Mouse monoclonal [C502S] to Caspase-8 p10 -
由来種
Mouse -
特異性
This antibody recognizes the 10 kDa small subunit of Caspase 8. Does not detect full-length caspase 8. -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human Caspase-8 p10 (N terminal).
-
特記事項
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
モノクローナル -
クローン名
C502S -
ミエローマ
unknown -
アイソタイプ
IgG1 -
軽鎖の種類
unknown -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
ターゲット情報
-
機能
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. -
組織特異性
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. -
関連疾患
Caspase-8 deficiency -
配列類似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
ドメイン
Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. -
翻訳後修飾
Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes. -
細胞内局在
Cytoplasm. - Information by UniProt
-
参照データベース
- Entrez Gene: 841 Human
- SwissProt: Q14790 Human
- Unigene: 599762 Human
-
別名
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch-5 antibody
- CAP4 antibody
see all
プロトコール
参考文献 (3)
ab2553 は 3 報の論文で使用されています。
- Jiao P et al. The protective effect of doxofylline against lipopolysaccharides (LPS)-induced activation of NLRP3 inflammasome is mediated by SIRT1 in human pulmonary bronchial epithelial cells. Artif Cells Nanomed Biotechnol 48:687-694 (2020). PubMed: 32098511
- Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405
- Helmke C et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res 26:914-34 (2016). PubMed: 27325299