Key features and details
- Mouse monoclonal [C502S] to Caspase-8 p10
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG1
製品名Anti-Caspase-8 p10 antibody [C502S]
製品の詳細Mouse monoclonal [C502S] to Caspase-8 p10
特異性This antibody recognizes the 10 kDa small subunit of Caspase 8. Does not detect full-length caspase 8.
アプリケーション適用あり: IP, WBmore details
Synthetic peptide corresponding to Human Caspase-8 p10 (N terminal).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.02% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab2553 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 µg/ml.|
機能Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
組織特異性Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
配列類似性Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains.
ドメインIsoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
翻訳後修飾Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
- Information by UniProt
- Apoptotic cysteine protease antibody
- Apoptotic protease Mch-5 antibody
- CAP4 antibody
ab2553 は 2 報の論文で使用されています。
- Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405
- Helmke C et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res 26:914-34 (2016). PubMed: 27325299