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  1. Link

    caspase-8-p10-antibody-c502s-ab2553.pdf

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Cell Biology Apoptosis Intracellular Caspases etc Caspases
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Anti-Caspase-8 p10 抗体 [C502S] (ab2553)

  • Datasheet
  • SDS
Submit a review Q&A (7)References (3)

Product price, shipping and contact information

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出荷および受注について

Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Key features and details

  • Mouse monoclonal [C502S] to Caspase-8 p10
  • Reacts with: Human
  • Isotype: IgG1

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

関連製品

医薬用外毒物

製品の概要

  • 製品名

    Anti-Caspase-8 p10 antibody [C502S]
  • 製品の詳細

    Mouse monoclonal [C502S] to Caspase-8 p10
  • 由来種

    Mouse
  • 特異性

    This antibody recognizes the 10 kDa small subunit of Caspase 8. Does not detect full-length caspase 8.
  • 種交差性

    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to Human Caspase-8 p10 (N terminal).

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー

    Preservative: 0.02% Thimerosal (merthiolate)
    Constituents: PBS, 50% Glycerol, 1% BSA
  • Concentration information loading...
  • 精製度

    Immunogen affinity purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    C502S
  • ミエローマ

    unknown
  • アイソタイプ

    IgG1
  • 軽鎖の種類

    unknown
  • 研究分野

    • Cell Biology
    • Apoptosis
    • Intracellular
    • Caspases etc
    • Caspases
    • Cancer
    • Invasion/microenvironment
    • Apoptosis
    • Caspases
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Apoptosis Markers
    • Caspases
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

ターゲット情報

  • 機能

    Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.
  • 組織特異性

    Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.
  • 関連疾患

    Caspase-8 deficiency
  • 配列類似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.
  • ドメイン

    Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.
  • 翻訳後修飾

    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.
  • 細胞内局在

    Cytoplasm.
  • Target information above from: UniProt accession Q14790 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 841 Human
    • SwissProt: Q14790 Human
    • Unigene: 599762 Human
    • 別名

      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch-5 antibody
      • CAP4 antibody
      • CASP-8 antibody
      • CASP8 antibody
      • CASP8_HUMAN antibody
      • Caspase 8 antibody
      • Caspase-8 subunit p10 antibody
      • FADD-homologous ICE/CED-3-like protease antibody
      • FADD-like ICE antibody
      • FLICE antibody
      • ICE-like apoptotic protease 5 antibody
      • MACH antibody
      • MORT1-associated CED-3 homolog antibody
      see all

    プロトコール

    • Western blot protocols
    • Immunoprecipitation protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
    • SDS
  • 参考文献 (3)

    ab2553 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2553 は 3 報の論文で使用されています。

    • Jiao P  et al. The protective effect of doxofylline against lipopolysaccharides (LPS)-induced activation of NLRP3 inflammasome is mediated by SIRT1 in human pulmonary bronchial epithelial cells. Artif Cells Nanomed Biotechnol 48:687-694 (2020). PubMed: 32098511
    • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405
    • Helmke C  et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res 26:914-34 (2016). PubMed: 27325299

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-7 of 7 Abreviews or Q&A

    Question

    Thank you for your explanation. In the case of using a mouse Ab in IHC for mouse tissues, one can label the primary Ab with Biotin to avoid erroneous results. Your antibody is the only one I can found that only recognize cleaved caspase-8 fragment. So do think it is reasonable to try this one on mouse tissue section? Thank you.

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2004

    Answer

    Thank you for your message. As it has not been tested yet with mouse tissue, we cannot provide any guarantees, but we would be very interested to hear how it works out for you if you do decide to try it. If you have any more questions, please do contact us again.

    Read More

    Abcam Scientific Support

    Answered on May 04 2004

    Question

    Hi, From reading some of the earlier enquiries, I understand that this caspase-8 Ab (C502S) only recongnizes human p10 but not the proform of caspase-8. I am looking for a caspase-8 antibody that only recongnizes activated caspase-8 in tissue section by IHC. So my questions are: 1. Whether this anbibody can only recognize mouse p10 of Casp-8 as well 2. Whehther this antibody can be successfully used in either Paraffin sections or Frozen sections in mouse tissues.

    Read More

    Abcam community

    Verified customer

    Asked on May 03 2004

    Answer

    Ab2553 has only been tested so far for cross-reactivity with human and tested in Western blotting and IP. I would not recommend this antibody to try with mouse samples in IHC - For immunohistochemistry, the primary antibody should be raised in a species as phylogenetically different as possible to the species that your sample was obtained. This is to avoid the secondary antibody cross-reacting with endogenous immunoglobulins in the sample, leading to erroneous results. For instance, a primary antibody used to detect a protein in a sample from a mouse should not be raised in mouse or rat. A primary antibody raised in rabbit would be a more appropriate choice.

    Read More

    Abcam Scientific Support

    Answered on May 03 2004

    Question

    Do these antibodies recognize both the active and the inactive forms of Caspase or only the active form? Could you please confirm it?

    Read More

    Abcam community

    Verified customer

    Asked on Mar 30 2004

    Answer

    Thank you for your enquiry. This antibody recognizes only the active form.

    Read More

    Abcam Scientific Support

    Answered on Apr 02 2004

    Question

    I want to confirm that the sequence I am using is actually from human and not mouse caspase as you seem to think, in which case would you mind double checking that our sequences are not the same? Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Sep 23 2003

    Answer

    The information I have on file and can release is that the immunogen is a 12 a.a peptide corresponding to the N-terminus of the p10 subunit of human caspase-8. I cannot release the exact sequence, but if customer is working on caspases, he or she can easily obtain the sequence by checking the caspase-8 sequence at clevage site.

    Read More

    Abcam Scientific Support

    Answered on Sep 24 2003

    Question

    Thank you for your previous information on this enquiry. The customer understands that you m,ay not wish to reveal your sequence used but wondered if you tell her if her sequence (LSSPQTRYIPDE) is not the same. thanks

    Read More

    Abcam community

    Verified customer

    Asked on Sep 22 2003

    Answer

    The customer's sequence seems to be derived from mouse caspase-8. ab2553 utilizes the sequence derived from human. There are a few amino acids are different between human and mouse caspase-8.

    Read More

    Abcam Scientific Support

    Answered on Sep 23 2003

    Question

    Thank you very much for the information. I have just placed an order for this Ab via my accounts department. Please could you tell me the sequence of the immunogen used to produce the Ab.

    Read More

    Abcam community

    Verified customer

    Asked on Sep 19 2003

    Answer

    The immunogen is the synthetic peptide corresponding to residues mapping at the N-terminus of the p10 fragment of caspase-8. Gloria

    Read More

    Abcam Scientific Support

    Answered on Sep 22 2003

    Question

    Caspase 8 antibody (ab2553) clone C502S. Does this Ab detect the p10 only or does it also detect the proform and the other cleaved fragments? I am interested in an Ab which only detects p10 .

    Read More

    Abcam community

    Verified customer

    Asked on Sep 18 2003

    Answer

    Yes, the antibody only detects p10 fragment of the cleaved caspase-8.

    Read More

    Abcam Scientific Support

    Answered on Sep 19 2003

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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