Anti-Caspase-8 p10 抗体 [C502S] (ab2553)

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Key features and details

  • Mouse monoclonal [C502S] to Caspase-8 p10
  • Suitable for: IP, WB
  • Reacts with: Human
  • Isotype: IgG1

製品の概要

  • 製品名

    Anti-Caspase-8 p10 antibody [C502S]

  • 製品の詳細

    Mouse monoclonal [C502S] to Caspase-8 p10

  • 由来種

    Mouse

  • 特異性

    This antibody recognizes the 10 kDa small subunit of Caspase 8. Does not detect full-length caspase 8.

  • アプリケーション

    適用あり: IP, WBmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Synthetic peptide corresponding to Human Caspase-8 p10 (N terminal).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab2553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IP Use a concentration of 10 µg/ml.
WB Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能

    Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

  • 組織特異性

    Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

  • 関連疾患

    Caspase-8 deficiency

  • 配列類似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.

  • ドメイン

    Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.

  • 翻訳後修飾

    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylation on Ser-387 during mitosis by CDK1 inhibits activation by proteolysis and prevents apoptosis. This phosphorylation occurs in cancer cell lines, as well as in primary breast tissues and lymphocytes.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch-5 antibody
      • CAP4 antibody
      • CASP-8 antibody
      • CASP8 antibody
      • CASP8_HUMAN antibody
      • Caspase 8 antibody
      • Caspase-8 subunit p10 antibody
      • FADD-homologous ICE/CED-3-like protease antibody
      • FADD-like ICE antibody
      • FLICE antibody
      • ICE-like apoptotic protease 5 antibody
      • MACH antibody
      • MORT1-associated CED-3 homolog antibody
      see all

    参考文献

    ab2553 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab2553 は 2 報の論文で使用されています。

    • Kaul Z & Chakrabarti O Tumor susceptibility gene 101 regulates predisposition to apoptosis via ESCRT machinery accessory proteins. Mol Biol Cell 28:2106-2122 (2017). PubMed: 28539405
    • Helmke C  et al. Ligand stimulation of CD95 induces activation of Plk3 followed by phosphorylation of caspase-8. Cell Res 26:914-34 (2016). PubMed: 27325299

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Question

    Thank you for your explanation. In the case of using a mouse Ab in IHC for mouse tissues, one can label the primary Ab with Biotin to avoid erroneous results. Your antibody is the only one I can found that only recognize cleaved caspase-8 fragment. So do think it is reasonable to try this one on mouse tissue section? Thank you.

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    Answer

    Thank you for your message. As it has not been tested yet with mouse tissue, we cannot provide any guarantees, but we would be very interested to hear how it works out for you if you do decide to try it. If you have any more questions, please do contact us again.

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    Question

    Hi, From reading some of the earlier enquiries, I understand that this caspase-8 Ab (C502S) only recongnizes human p10 but not the proform of caspase-8. I am looking for a caspase-8 antibody that only recongnizes activated caspase-8 in tissue section by IHC. So my questions are: 1. Whether this anbibody can only recognize mouse p10 of Casp-8 as well 2. Whehther this antibody can be successfully used in either Paraffin sections or Frozen sections in mouse tissues.

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    Answer

    Ab2553 has only been tested so far for cross-reactivity with human and tested in Western blotting and IP. I would not recommend this antibody to try with mouse samples in IHC - For immunohistochemistry, the primary antibody should be raised in a species as phylogenetically different as possible to the species that your sample was obtained. This is to avoid the secondary antibody cross-reacting with endogenous immunoglobulins in the sample, leading to erroneous results. For instance, a primary antibody used to detect a protein in a sample from a mouse should not be raised in mouse or rat. A primary antibody raised in rabbit would be a more appropriate choice.

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    Question

    Do these antibodies recognize both the active and the inactive forms of Caspase or only the active form? Could you please confirm it?

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    Answer

    Thank you for your enquiry. This antibody recognizes only the active form.

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    Question

    I want to confirm that the sequence I am using is actually from human and not mouse caspase as you seem to think, in which case would you mind double checking that our sequences are not the same? Thanks

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    Answer

    The information I have on file and can release is that the immunogen is a 12 a.a peptide corresponding to the N-terminus of the p10 subunit of human caspase-8. I cannot release the exact sequence, but if customer is working on caspases, he or she can easily obtain the sequence by checking the caspase-8 sequence at clevage site.

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    Question

    Thank you for your previous information on this enquiry. The customer understands that you m,ay not wish to reveal your sequence used but wondered if you tell her if her sequence (LSSPQTRYIPDE) is not the same. thanks

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    Answer

    The customer's sequence seems to be derived from mouse caspase-8. ab2553 utilizes the sequence derived from human. There are a few amino acids are different between human and mouse caspase-8.

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    Question

    Thank you very much for the information. I have just placed an order for this Ab via my accounts department. Please could you tell me the sequence of the immunogen used to produce the Ab.

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    Answer

    The immunogen is the synthetic peptide corresponding to residues mapping at the N-terminus of the p10 fragment of caspase-8. Gloria

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    Question

    Caspase 8 antibody (ab2553) clone C502S. Does this Ab detect the p10 only or does it also detect the proform and the other cleaved fragments? I am interested in an Ab which only detects p10 .

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    Answer

    Yes, the antibody only detects p10 fragment of the cleaved caspase-8.

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