Anti-Caspase-8 抗体 (ab4052)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab4052)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab4052)

Key features and details

  • Rabbit polyclonal to Caspase-8
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG

リコンビナント抗体で、ロット間での高い再現性を実現

Product image
Anti-Pro Caspase-8 antibody [EPR162] (ab108333)
  • 異なるロット間での安定した再現性
  • 容易なスケールアップ
  • 評価試験による特異性の確認済み
  • 倫理基準に準拠 - アニマル・フリーの生産

製品の概要

  • 製品名

    Anti-Caspase-8 antibody
    Caspase-8 一次抗体 製品一覧

  • 製品の詳細

    Rabbit polyclonal to Caspase-8

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Recombinant full length protein corresponding to Human Caspase-8.
    Database link: Q14790

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4052の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (1)
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
特記事項
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能

    Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
    -AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

  • 組織特異性

    Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle.

  • 関連疾患

    Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization.

  • 配列類似性

    Belongs to the peptidase C14A family.
    Contains 2 DED (death effector) domains.

  • ドメイン

    Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex.

  • 翻訳後修飾

    Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
    Phosphorylated upon DNA damage, probably by ATM or ATR.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • ALPS2B antibody
      • Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein antibody
      • Apoptosis related cysteine peptidase antibody
      • Apoptotic cysteine protease antibody
      • Apoptotic protease Mch-5 antibody
      • Apoptotic protease Mch5 antibody
      • CAP 4 antibody
      • CAP4 antibody
      • CASP-8 antibody
      • CASP8 antibody
      • CASP8_HUMAN antibody
      • Caspase 8 antibody
      • Caspase 8 apoptosis related cysteine peptidase antibody
      • Caspase IIX antibody
      • Caspase-8 subunit p10 antibody
      • caspase8 antibody
      • CED 3 antibody
      • FADD Homologous ICE/CED3 Like Protease antibody
      • FADD Like ICE antibody
      • FADD-homologous ICE/CED-3-like protease antibody
      • FADD-like ICE antibody
      • FLICE antibody
      • FLJ17672 antibody
      • ICE-like apoptotic protease 5 antibody
      • MACH alpha 1/2/3 protein antibody
      • MACH antibody
      • MACH beta 1/2/3/4 protein antibody
      • MACH5 antibody
      • MCH 5 antibody
      • MCH5 antibody
      • MGC78473 antibody
      • MORT1 associated ced 3 homolog antibody
      • MORT1 associated CED3 homolog antibody
      • MORT1-associated CED-3 homolog antibody
      • OTTHUMP00000163717 antibody
      • OTTHUMP00000163720 antibody
      • OTTHUMP00000163724 antibody
      • OTTHUMP00000163725 antibody
      • OTTHUMP00000165062 antibody
      • OTTHUMP00000165063 antibody
      • OTTHUMP00000165064 antibody
      • OTTHUMP00000206552 antibody
      • OTTHUMP00000206582 antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab4052)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab4052)This image is courtesy of an Abreview submitted by Dr B Goilav

      ab4052 staining Caspase-8 in human kidney tissue section by immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue  underwent fixation in paraformaldehyde, heat mediated antigen retrieval in 10mM Citrate buffer pH 6.0, permeabilization in 0.1% Triton X-100 prior to blocking in 10% serum for 20 minutes at 22°C. The primary antibody, diluted 1/50 in PBS with 2% BSA and incubated with sample, for 45 minutes at 22°C. An Avidin-peroxidase conjugated horse polyclonal to rabbit Ig, was used undiluted as the secondary antibody.

      See Abreview

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab4052)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-8 antibody (ab4052)

      ab4052 staining Caspase-8 in formalin-fixed, paraffin-embedded Human stomach tissue by Immunohistochemistry.

    参考文献 (11)

    ab4052 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4052 は 11 報の論文で使用されています。

    • Buyuktiryaki M  et al. Therapeutic and preventative effects of ankaferd blood stopper in an experimental necrotizing enterocolitis model. Biomed Pharmacother 110:105-110 (2019). PubMed: 30465999
    • Isik S  et al. Sumac (Rhus coriaria) for the prevention and treatment of necrotizing enterocolitis. J Food Biochem 43:e13068 (2019). PubMed: 31583737
    • Chang CW  et al. Lactobacillus casei Variety rhamnosus Probiotic Preventively Attenuates 5-Fluorouracil/Oxaliplatin-Induced Intestinal Injury in a Syngeneic Colorectal Cancer Model. Front Microbiol 9:983 (2018). PubMed: 29867884
    • Cakir U  et al. Ginger (Zingiber officinale Roscoe) for the treatment and prevention of necrotizing enterocolitis. J Ethnopharmacol 225:297-308 (2018). PubMed: 30005955
    • Masalova OV  et al. Modulation of Cell Death Pathways by Hepatitis C Virus Proteins in Huh7.5 Hepatoma Cells. Int J Mol Sci 18:N/A (2017). PubMed: 29113144
    View all Publications for this product

    レビューと Q&A

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    1-7 of 7 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Caspase 8 antibody

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Kidney)
    Specification
    Kidney
    Fixative
    Paraformaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer, pH 6.0
    Permeabilization
    Yes - 0.1% Triton X-100
    Blocking step
    Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 22°C
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    Dr. B Goilav

    Verified customer

    投稿 Dec 18 2008

    Question

    I am not clear that whether ab4052(anti-caspase 8) will be recognizing full length caspase 8(pro-caspase 8). Does this antibody work for cell culture staining (IFC)? Thanks

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    Answer

    Thank you for contacting us.

    I'm afraid we are unable to determine if the antibody is specific to the active form only of caspase 8 or the pro-form as it has only been tested in IHC. We also haven't tested it in staining cells (ICC/IF). Most likely it will work, as it is a simliar application to IHC, but we can't guarantee ICC/IF since it is untested.

    I'm sorry I cannot be of further assistance on this occasion.

    Use our products? Submit an Abreview. Earn rewards!
    https://www.abcam.com/abreviews

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    Question

    Does the antibody recognise the active form only of caspase 8?

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    Answer

    I'm afraid we are unable to determine if the antibody is specific to the active form only of caspase 8, as it has only been tested in IHC. I'm sorry I cannot be of further assistance on this occasion.

    Read More

    Question

    Christian is visting Cambridge (and Abcam of course), so I forward our customers reply to your suggestion: "... Dear abcam-team Thank you for your kind reply concerning the antibody anti-Caspase 8 / Flice no. AB4052. I have used the PBST medium as diluent for the antibodies, but again failed to stain apoptotic cells. Further changes in our protocol were also not successful. I got no staining at all in any slide. You successfully used this antibody in immunohistochemistry in paraffin embedded murine tissue. Would it be possible to get your staining-protocol? It would help me very much. Thanks in advance J. Podlech .." Hoepfully you can provide such a protocol?

    Read More
    Answer

    I can confirm that the customer's protocol is adequate and should work. However, we have the following suggestions: 1. Try using another tissue besides liver. We recommend stomach. 2. The customer is using home brewed detection system. The sensitivity of the system may therefore not be adequate. I would suggest the followings: try using lower dilution of the primary antibody or try using more concentrated link (goat anti rabbit, 1:500 may be too diluted). Please let me know if the customer still has problems with the positive control and we can arrange for a replacement vial,

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    Question

    follow up to previous correspondance: The antibody was stored in our refrigerator (4°C) and used the other day, after delivery. The antigen retrieval step was performed as we usually do it. That means a boiling step of about 5 min and cooling for about 1 hour in the unmasking solution. It works very well with a lot of antibodies in our hands. A permeabilisation step was not done, because I don't think that this step is really necessary for formalin-fixed and paraffin-embedded tissue. The antibody was diluted in TBS. The immunohistochemistry protocol includes a serum block (10% goat serum in TBS) before incubation with the first antibody. I hope this further information is helpful. Thank you very much for your efforts.

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    Answer

    Thank you for those further protocol details, I would like to recommend diluting the blocking serum, primary and secondary antibodies in PBST containing triton x100 (0.3%v/v) to promote permeabilization of the tissue and enable better penetration of the antibody. Please let me know if you still have problems with this antibody and we can offer you a replacement or credit note or refund if you purchased the antibody in the last 90 days,

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    Question

    BATCH NUMBER 98717 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM This antibody does not work in immunohistochemistry with murine liver tissue fixes with 4% buffered formalin and embedded in paraffin with routine methods in our hands. We got no staining. SAMPLE Mouse Liver (whole organ) SECONDARY ANTIBODY anti-Rabbit conjugated with biotin, 1:500, from Sigma B8895, for 30 min at RT DETECTION METHOD the tissue used is positive with an anti-active Caspase 3 ab and serves generally for me as an apoptosis positive control with a lot of microscopically typical apoptotic cells (FAS induced) POSITIVE AND NEGATIVE CONTROLS USED FAS induced liver cell necroses FIXATION OF SAMPLE Formalin 4%, buffered with PBS to pH=7.4 ANTIGEN RETRIEVAL anti-Caspase 8, 0.5 mL vial, used in a 1:50 dilution in TBS for O/N at room temperature BLOCKING CONDITIONS enzymatic digestion with trypsin or without digestion, followed by the unmasking technique with citrate buffer before incubation with the primary antibody. Detection with the Avidin-Biotin-Complex-method (peroxidase) and DAB as the substrate after incubation with the secondary antibody. HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 4 HAVE YOU RUN A "NO PRIMARY" CONTROL? Yes DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes ADDITIONAL NOTES Other anti caspase antibody work well in this system

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    Answer

    I'm very sorry to hear you are having problems with ab4052, thank you for taking the time to fill in the online questionnaire. Could you please tell me: -how the antibody was stored? - how long the antigen retrieval step was? - if you have done a permeabilization step? - the buffer used to dilute the antibodies? -what blocking was done (BSA/serum? HRP block?)? My apologies for the delay, thank you for your patience, I look forward to hearing from you,

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    Question

    Will this product detect both the active an inactive form of caspase 8?

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    Answer

    Thank you for your enquiry. This antibody recognizes the full length inactive proenzyme (55 kDa). While this antibody has also been shown to detect the processed forms of Caspase 8 (which associate to form the active enzyme), its specific reaction with the active form is unknown to us. If you have any further questions then please do not hesitate to get back in touch with us.

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