Caspase-8 (active) FITC Staining Kit (ab65614)
Key features and details
- Assay type: Enzyme activity
- Platform: Microplate reader, Fluor. microscope, Flow cyt.
- Assay time: 2 hr
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Caspase-8 (active) FITC Staining Kit
Caspase-8 キット 製品一覧 -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Enzyme activity -
全工程の試験時間
2h 00m -
製品の概要
Caspase 8 (active) FITC Staining Kit (ab65614) provides a convenient means for sensitive detection of activated caspase 8 in living cells. The assay utilizes the caspase 8 inhibitor, IETD-FMK, conjugated to FITC (FITC-IETD-FMK) as a marker. FITC-IETD-FMK is cell permeable, non-toxic, and irreversibly binds to activated caspase 8 in apoptotic cells. The FITC label allows detection of activated caspase-8 in apoptotic cells directly by fluorescence microscopy, flow cytometry, or fluorescence plate reader.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K188 CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit. K188-100 is the same size as the 100 test size of ab65614.
Activation of caspases plays a central role in apoptosis.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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試験プラットフォーム
Microplate reader, Fluor. microscope, Flow cyt.
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests FITC-IETD-FMK 1 x 100µl Wash Buffer IV 2 x 100ml Z-VAD-FMK 1 x 10µl -
研究分野
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機能
Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-
-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex. -
組織特異性
Isoform 1, isoform 5 and isoform 7 are expressed in a wide variety of tissues. Highest expression in peripheral blood leukocytes, spleen, thymus and liver. Barely detectable in brain, testis and skeletal muscle. -
関連疾患
Defects in CASP8 are the cause of caspase-8 deficiency (CASP8D) [MIM:607271]. CASP8D is a disorder resembling autoimmune lymphoproliferative syndrome (ALPS). It is characterized by lymphadenopathy, splenomegaly, and defective CD95-induced apoptosis of peripheral blood lymphocytes (PBLs). It leads to defects in activation of T-lymphocytes, B-lymphocytes, and natural killer cells leading to immunodeficiency characterized by recurrent sinopulmonary and herpes simplex virus infections and poor responses to immunization. -
配列類似性
Belongs to the peptidase C14A family.
Contains 2 DED (death effector) domains. -
ドメイン
Isoform 9 contains a N-terminal extension that is required for interaction with the BCAP31 complex. -
翻訳後修飾
Generation of the subunits requires association with the death-inducing signaling complex (DISC), whereas additional processing is likely due to the autocatalytic activity of the activated protease. GZMB and CASP10 can be involved in these processing events.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Cytoplasm. - Information by UniProt
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別名
- ALPS2B
- Amyotrophic lateral sclerosis 2 chromosomal region candidate gene 12 protein
- Apoptosis related cysteine peptidase
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関連製品
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Related Products
画像
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Active caspase 8 in Jurkat cells following four hours exposure to 50 ng/mL anti-Fas Ab (αFas) (MBL), five hours with 10 μg/mL cyclohexamide (CHX) (ab120093), or one hour pretreatment with CHX followed by four hours with αFas. Background signal subtracted, duplicates; +/- SD.
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Caspase 8 activation of Hela (left) and SiHa (right) cells after in vitro treatment with pentoxylline (PTX) or cisplatin (CIS) either alone or in combination. Results represent the mean ± SD of three independent experiments carried out in triplicate. (*) p<0.001 vs CTL.
Image obtained from Hernandez-Flores G et al; BMC Cancer, 2011 Nov 11; 11:483
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab65614 は 6 報の論文で使用されています。
- Lai JKH et al. DNA-damage induced cell death in yap1;wwtr1 mutant epidermal basal cells. Elife 11:N/A (2022). PubMed: 35635436
- Park JH et al. Evaluation of moxifloxacin-induced cytotoxicity on human corneal endothelial cells. Sci Rep 11:6250 (2021). PubMed: 33737688
- Ali H et al. Selective killing of human M1 macrophages by Smac mimetics alone and M2 macrophages by Smac mimetics and caspase inhibition. J Leukoc Biol 110:693-710 (2021). PubMed: 33404106
- Ding D et al. Some Ototoxic Drugs Destroy Cochlear Support Cells Before Damaging Sensory Hair Cells. Neurotox Res 37:743-752 (2020). PubMed: 31997155
- Povo-Retana A et al. Specific Effects of Trabectedin and Lurbinectedin on Human Macrophage Function and Fate-Novel Insights. Cancers (Basel) 12:N/A (2020). PubMed: 33092171
- Hernandez-Flores G et al. Pentoxifylline sensitizes human cervical tumor cells to cisplatin-induced apoptosis by suppressing NF-kappa B and decreased cell senescence. BMC Cancer 11:483 (2011). PubMed: 22074157