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遺伝子ノックアウト細胞株 検証済リコンビナントRabMAb

Anti-Caspase-6/CASP-6 抗体 [EPR4405] (ab108335)

Reviews (3) Submit a question References (6)
Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
  • Flow Cytometry (Intracellular) - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
  • Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6
  • Suitable for: Flow Cyt (Intra), WB
  • Knockout validated
  • Reacts with: Mouse, Human

Conjugates logo Related conjugates and formulations

Carrier Free

製品の概要

  • 製品名

    Anti-Caspase-6/CASP-6 antibody [EPR4405]
    Caspase-6/CASP-6 一次抗体 製品一覧

  • 製品の詳細

    Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6

  • 由来種

    Rabbit

  • 特異性

    ab108335 detects both the full length and cleaved forms of Caspase-6/CASP-6.

  • アプリケーション

    適用あり: Flow Cyt (Intra), WBmore details
    適用なし: ICC or IHC-P

  • 種交差性

    交差種: Mouse, Human
    交差が予測される動物種: Rat

  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • ポジティブ･コントロール

    • Jurkat cells and cell lysate.

  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab108335の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
特記事項
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.  
WB
1/1000 - 1/10000. Predicted molecular weight: 33 kDa.
追加情報
Is unsuitable for ICC or IHC-P.

ターゲット情報

  • 機能

    Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves poly(ADP-ribose) polymerase in vitro, as well as lamins. Overexpression promotes programmed cell death.

  • 配列類似性

    Belongs to the peptidase C14A family.

  • 翻訳後修飾

    Cleavages by caspase-3, caspase-8 or -10 generate the two active subunits.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Apoptotic protease Mch-2 antibody
    • Apoptotic protease MCH2 antibody
    • CASP-6 antibody
    • CASP6 antibody
    • CASP6_HUMAN antibody
    • Caspase 6 antibody
    • Caspase 6 apoptosis related cysteine protease antibody
    • Caspase 6, apoptosis related cysteine peptidase antibody
    • Caspase-6 antibody
    • Caspase-6 subunit p11 antibody
    • Mch2 antibody
    • OTTHUMP00000162742 antibody
    • OTTHUMP00000162743 antibody
    see all

画像

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : CASP6 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 33 kDa



    Lanes 1 - 2: Merged signal (red and green). Green - ab108335 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.

    ab108335 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CASP6 knockout cells. Wild-type and CASP6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab108335 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
    Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
    All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat + staurosporine cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 33 kDa

  • Flow Cytometry (Intracellular) - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
    Flow Cytometry (Intracellular) - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

    ab108335, at 1/10 dilution, staining Caspase-6/CASP-6 in permeabilized Jurkat cells by intracellular flow cytometry (shown in red). Rabbit IgG shown in green (negative). 

     

     

  • Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)
    Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

参考文献 (6)

ab108335 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab108335 は 6 報の論文で使用されています。

  • Zhu Y  et al. Cardamonin inhibits cell proliferation by caspase-mediated cleavage of Raptor. Naunyn Schmiedebergs Arch Pharmacol 394:809-817 (2021). PubMed: 33043385
  • Wang Y  et al. Network Pharmacology-Based Strategy for the Investigation of the Anti-Obesity Effects of an Ethanolic Extract of Zanthoxylum bungeanum Maxim. Front Pharmacol 11:572387 (2020). PubMed: 33364948
  • Mou Z  et al. Transcriptomic Analysis of Glycolysis-Related Genes Reveals an Independent Signature of Bladder Carcinoma. Front Genet 11:566918 (2020). PubMed: 33424916
  • Volpin V  et al. CAMK1D Triggers Immune Resistance of Human Tumor Cells Refractory to Anti-PD-L1 Treatment. Cancer Immunol Res 8:1163-1179 (2020). PubMed: 32665263
  • Chen YJ  et al. Nitrite Protects Neurons Against Hypoxic Damage Through S-nitrosylation of Caspase-6. Antioxid Redox Signal 31:109-126 (2019). PubMed: 30417658
  • Ma J  et al. Reversing drug resistance of soft tumor-repopulating cells by tumor cell-derived chemotherapeutic microparticles. Cell Res 26:713-27 (2016). PubMed: 27167569

レビューと Q&A

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1-3 of 3 Abreviews or Q&A

Flow Cytometry abreview for Anti-Caspase-6/CASP-6 antibody [EPR4405]

 Good
Abreviews
Application
Flow Cytometry
Sample
Mouse Cell (ledyig cells)
Permeabilization
Yes - 0.1% PBS-Tween
Gating Strategy
RLF positive cells
Specification
ledyig cells
Preparation
Cell harvesting/tissue preparation method: Tubules from testes were incubated for 15–20 min at 35°C in a shaking water bath at 80 oscillations (osc)/min , then layered over 40 mL 5% Percoll and leydig cells were isolated from top of the percoll
Sample buffer: 0.1% PBS BSA
Fixation
Formaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

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投稿 Dec 04 2013

Flow Cytometry abreview for Anti-Caspase-6/CASP-6 antibody [EPR4405]

 Good
Abreviews
Application
Flow Cytometry
Sample
Human Cell (MCF7)
Permeabilization
Yes - 0.1% PBS-Tween
Gating Strategy
CD44 POSITIVE
Specification
MCF7
Preparation
Cell harvesting/tissue preparation method: treated with collagenase and trypsin to make single cell suspension
Sample buffer: 0.1% PBS BSA
Fixation
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 04 2013

Flow Cytometry abreview for Anti-Caspase-6 / CASP-6 antibody [EPR4405]

 Good
Abreviews
Application
Flow Cytometry
Sample
Rat Cell (breast cells)
Permeabilization
Yes - 0.1% PBS-Tween
Gating Strategy
cd1d1 POSITIVE
Specification
breast cells
Preparation
Cell harvesting/tissue preparation method: tissue was treated with collagenase to make single cell suspension
Sample buffer: 0.1% PBS BSA
Fixation
Methanol
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 04 2013

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