Caspase 5 Assay Kit (Colorimetric) (ab65661)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Tissue Extracts
製品の概要
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製品名
Caspase 5 Assay Kit (Colorimetric)
Caspase-5 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Tissue Extracts, Cell Lysate -
アッセイタイプ
Enzyme activity -
全工程の試験時間
2h 00m -
製品の概要
Abcam's Caspase 5 Assay Kit (Colorimetric) provides a simple and convenient means for assaying the activity of caspases that recognize the sequence WEHD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (p-NA) after cleavage from the labeled substrate WEHD-p-NA. The p-NA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K123 Caspase-5 Colorimetric Assay Kit. K123-100 is the same size as the 100 test size of ab65661.
Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells.
Other caspase and apoptosis assays
Review the full set of caspase assays, or the apoptosis assay and apoptosis marker guide.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2X Reaction Buffer 4 x 2ml Cell Lysis Buffer 1 x 100ml Dilution Buffer II 1 x 100ml DTT 1 x 400µl WEHD-pNA (4mM) 1 x 500µl -
研究分野
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関連性
Caspase 5 belongs to the Ced3/ICE family of cysteine proteases. Caspase 5 exists as an inactive proenzyme which undergoes proteolytic processing at conserved aspartic residues, leading to p20 and p10 subunits that dimerize to form the active enzyme. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. -
別名
- CASP 5
- CASP5
- Caspase 5 apoptosis related cysteine peptidase
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関連製品
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab65661 は論文での使用が確認できていません。