Caspase-4 Inhibitor Assay Kit (ab102492)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
製品の概要
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製品名
Caspase-4 Inhibitor Assay Kit -
検出方法
Fluorescent -
サンプルの種類
Adherent cells, Suspension cells -
アッセイタイプ
Quantitative -
全工程の試験時間
1h 30m -
製品の概要
Abcam's Caspase 4 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 4 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 4 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting. -
特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K154 Caspase-4 Inhibitor Drug Screening Kit (Fluorometric). K154-100 is the same size as the 100 test size of ab102492.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-4 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate LEVD-AFC 1 x 0.5ml DTT 1 x 100µl -
研究分野
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関連性
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts. -
別名
- CASP4 inhibitor
- Caspase 4 inhibitor
関連製品
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Related Products
画像
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Caspase 4 Inhibitor Drug Detection Kit (ab102492)
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEVD-AFC as caspase 4 substrate and following Caspase 4 Inhibitor Drug Detection Kit (ab102492) protocol. Lanes:
1.- Background Control: no Caspase 4, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 4, +z-VAD-FMK
3.- Positive Control: + Caspase 4, no z-VAD-FMK
4.- Caspase 4 + 2.5µM z-VAD-FMK
5.- Caspase 4 + 10µM z-VAD-FMK
6.- Caspase 4 + 40µM z-VAD-FMK
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab102492 は論文での使用が確認できていません。