Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Adherent cells, Suspension cells
製品名Caspase-4 Inhibitor Assay Kit
サンプルの種類Adherent cells, Suspension cells
Abcam's Caspase 4 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate LEVD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase 4 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase 4 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
Visit our FAQs page for tips and troubleshooting.
This product is manufactured by BioVision, an Abcam company and was previously called K154 Caspase-4 Inhibitor Drug Screening Kit (Fluorometric). K154-100 is the same size as the 100 test size of ab102492.
Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
保存方法Store at -20°C. Please refer to protocols.
内容 100 tests 2X Reaction Buffer 1 x 10ml Active Caspase-4 1 x 100 units Caspase Inhibitor, Z-VAD-FMK 1 x 10µl Caspase Substrate LEVD-AFC 1 x 0.5ml DTT 1 x 100µl
関連性Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.
- CASP4 inhibitor
- Caspase 4 inhibitor
Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using LEVD-AFC as caspase 4 substrate and following Caspase 4 Inhibitor Drug Detection Kit (ab102492) protocol. Lanes:
1.- Background Control: no Caspase 4, no z-VMAD-FMK
2.- Positive Inhibition Control: no Caspase 4, +z-VAD-FMK
3.- Positive Control: + Caspase 4, no z-VAD-FMK
4.- Caspase 4 + 2.5µM z-VAD-FMK
5.- Caspase 4 + 10µM z-VAD-FMK
6.- Caspase 4 + 40µM z-VAD-FMK