Caspase 3/7 Staining Kit (Far Red) (ab270785)
製品の概要
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製品名
Caspase 3/7 Staining Kit (Far Red) -
製品の概要
Caspases play important roles in apoptosis and inflammation. Caspase 3/7 Staining Kit (Far Red) are used by researchers seeking to quantitate apoptosis via caspase activity in cultured cells. The fluorescent 660-DEVD-FMK caspase-3/7 inhibitor reagent allows researchers to assess caspase activation.
The fluorescent reagent 660-DEVD-FMK enters each cell and irreversibly binds to activated Caspase 3/7. Because the 660-DEVD-FMK reagent becomes covalently coupled to the active enzymes, it is retained within the cell, while any unbound 660-DEVD-FMK reagent diffuses out of the cell and is washed away. The remaining far red fluorescent signal is a direct measure of the active caspase enzyme activity present in the cell at the time the reagent was added. Cells that contain the bound 660-DEVD-FMK can be analyzed by fluorescence microscopy or flow cytometry. Cells labeled with the 660-DEVD-FMK reagent may be read immediately or preserved for 16 hours using the fixative. Unfixed samples may also be analyzed with Hoechst stain to detect changes in nuclear morphology.
Produced using a non-baculovirus expression system.
The target for this kit is Caspase 3/7.
NOTE: The number of tests per kit depends on the application being tested. This kit provides 50 tests for flow and 25 for imaging.
FLICA 660-DEVD-FMK caspase-3/7 inhibitor reagent contains the preferred binding sequence, Asp-Glu-Val-Asp (DEVD) for executioner caspases -3 and -7. This preferred caspase-3 and -7 binding sequence, DEVD, is labeled at the amino terminus end with a far-red fluorescent 660 dye and linked at the carboxyl end to a fluoromethyl ketone (FMK) reactive entity. The resulting cell permeant, fluorescent molecule, 660-DEVD-FMK, optimally excites at 660 nm and emits between 685-690 nm. A conventional red HeNe laser with a 633 nm excitation provides excellent excitation efficiency, enabling cells labeled with 660-DEVD-FMK to be analyzed with most flow cytometers, as well as fluorescence microscopes equipped with electronic grey scale image capabilities.
Kits are available for other caspases:
Poly Caspase Staining Kit (Far Red) (ab270783)
Caspase 1 Staining Kit (Far Red) (ab27084)
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アプリケーション
適用あり: FM, Flow Cytmore details
製品の特性
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保存方法
Store at +4°C. Please refer to protocols. -
内容 50 tests 10X Apoptosis Wash Buffer 1 x 15ml Fixative 1 x 6ml 660-DEVD-FMK caspase-3/7 inhibitor reagent 1 vial
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab270785の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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FM |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
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特記事項 |
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FM
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. |
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab270785 は 2 報の論文で使用されています。
- Krach F et al. Aberrant NOVA1 function disrupts alternative splicing in early stages of amyotrophic lateral sclerosis. Acta Neuropathol 144:413-435 (2022). PubMed: 35778567
- Krach F et al. An alternative splicing modulator decreases mutant HTT and improves the molecular fingerprint in Huntington's disease patient neurons. Nat Commun 13:6797 (2022). PubMed: 36357392