Application
Flow Cytometry
Sample
Human Cell (PBMCs)
Permeabilization
Yes - perm buffer
Gating Strategy
Gate on Lymphozytes and monocytes
Specification
PBMCs
Preparation
Cell harvesting/tissue preparation method: Ficoll density separation
Sample buffer: PBS
Sample buffer: PBS
Fixation
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Staining buffer
Dilution
1/500
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: Goat anti rabbit AF488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
a) untreated cells
b) cells treated with staurosporine (5µM) for 16hours
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投稿 Jan 25 2020