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Caspase-1 Inhibitor Assay Kit (ab102489)

Submit a review Q&A (1)References (1)
Caspase 1 Inhibitor Drug Detection Kit (ab102489)

    Key features and details

    • Assay type: Quantitative
    • Detection method: Fluorescent
    • Platform: Microplate reader
    • Assay time: 1 hr 30 min
    • Sample type: Adherent cells, Suspension cells

    製品の概要

    • 製品名

      Caspase-1 Inhibitor Assay Kit
      Caspase-1 キット 製品一覧

    • 検出方法

      Fluorescent

    • サンプルの種類

      Adherent cells, Suspension cells

    • アッセイタイプ

      Quantitative

    • 全工程の試験時間

      1h 30m

    • 製品の概要

      Abcam's Caspase 1 Inhibitor Drug Detection Kit provides an effective means for screening caspase inhibitors using fluorometric methods. The assay utilizes synthetic peptide substrate YVAD-AFC (AFC, 7-amino-4-trifluoromethyl coumarin). Active caspase-1 cleaves the synthetic substrate to release free AFC which can then be quantified by fluorometry. Compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison of the fluorescence intensity in samples with and without the testing inhibitors.
      Visit our FAQs page for tips and troubleshooting.

    • 特記事項

      This product is manufactured by BioVision, an Abcam company and was previously called K151 Caspase-1 Inhibitor Drug Screening Kit (Fluorometric). K151-100 is the same size as the 100 test size of ab102489.

      Caspases have been shown to play a crucial role in apoptosis induced by various deleterious and physiologic stimuli. Inhibition of caspases can delay apoptosis, implicating a potential role in drug screening efforts.

    • 試験プラットフォーム

      Microplate reader

    製品の特性

    画像

    • Caspase 1 Inhibitor Drug Detection Kit (ab102489)
      Caspase 1 Inhibitor Drug Detection Kit (ab102489)

      Caspase activity (RFU) in presence of 0µM - 40µM of z-VAD-FMK (generic caspase inhibitor), assessed using YVAD-AFC as caspase 1 substrate and following Caspase 1 Inhibitor Drug Detection Kit (ab102489) protocol. Lanes:

      1.- Background Control: no Caspase 1, no z-VMAD-FMK

      2.- Positive Inhibition Control: no Caspase 1, +z-VAD-FMK

      3.- Positive Control: + Caspase 1, no z-VAD-FMK

      4.- Caspase 1 + 2.5µM z-VAD-FMK

      5.- Caspase 1 + 10µM z-VAD-FMK

      6.- Caspase 1 + 40µM z-VAD-FMK

    参考文献 (1)

    ab102489 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab102489 は 1 報の論文で使用されています。

    • Gren ST  et al. The protease inhibitor cystatin C down-regulates the release of IL-ß and TNF-a in lipopolysaccharide activated monocytes. J Leukoc Biol 100:811-822 (2016). PubMed: 27190304

    レビューと Q&A

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    Question

    ab102489
    Cell preparation - is this to test cell culture (cell free assay) or cells themselves

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    Answer

    Thank you for your telephone enquiry.

    I can confirm this kit is designed to screen caspase-1 inhibitors. This can be done in 2 ways:

    Putative caspase-1 inhibitors used to treat cells expressing caspase-1 can be compared for the inhibition potential. This assay does not need live cells but cell lysate can be used. In this case, cells can be parallel treated with the model inhibitor Z-VAD-FMK for comparison.

    The kit comes with an active caspase-1 and compounds to be screened can directly be added to the reaction and the level of inhibition of caspase-1 activity can be determined by comparison. Z-VAD-FMK can be used to compare other inhibitors of interest.


    Citation: Wei Y. et al. (2006) Am. J. Physiol. Endocrinol. Metab. E 275-E281.


    Hope this information is helpful. Please do not hesitate to contact us if you have any further questions or concerns.

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